LOCUS V030888 8583 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030888 VERSION V030888 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4730 /label="Map4k4(NM_008696)" /note="Map4k4(NM_008696)" /gene="Map4k4" CDS 4749..4778 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4797..4820 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4840..4856) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4867..5489 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5638..6226) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6555..6602 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6834..7628) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7663..8020) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(8022..8126) /gene="bla" /label="AmpR promoter" rep_origin 8153..8581 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggcgaacgac tctcccgcga agagcctggt ggacattgac ctgtcgtccc 1081 tgcgggaccc tgctgggatt tttgagctgg tggaagtggt tggaaatggc acctatggac 1141 aagtctataa gggtcgacat gttaaaacgg gtcagctggc cgccatcaag gttatggacg 1201 tcaccgagga tgaagaggaa gaaatcaaac tggagataaa tatgctgaag aagtattctc 1261 atcatcgaaa tattgccacg tactatggtg ctttcattaa gaagagccct ccaggacatg 1321 atgaccaact ctggcttgtt atggagtttt gtggggctgg gtccatcaca gaccttgtga 1381 agaacaccaa agggaacact ctcaaagaag actggattgc ttacatctcc agggaaatcc 1441 tcaggggatt ggcacatctc catattcacc acgttattca ccgagatatc aagggccaaa 1501 atgtgctgct gaccgagaat gctgaggtga aacttgttga ttttggtgta agcgctcagc 1561 tggacaggac ggttggacgg agaaatacgt tcataggcac accctactgg atggctccag 1621 aggtcatcgc ctgtgatgag aacccagacg ccacttacga ctacagaagt gacctctggt 1681 cctgtggcat cacagccatc gagatggctg aaggggcccc ccctctctgt gacatgcatc 1741 caatgagagc gctgtttctc atccccagaa accctcctcc caggctgaag tcaaaaaaat 1801 ggtcaaagaa atttttcagc tttatagaag gctgtctggt gaagaattac atgcagcggc 1861 cctctacaga gcaactttta aaacaccctt tcataaggga tcagcccaat gaaaggcagg 1921 ttcgaatcca gcttaaggat cacatagacc ggaccagaaa gaagagaggc gagaaagatg 1981 agacggagta cgagtacagc gggagcgagg aggaggagga ggaagtgcct gagcaggagg 2041 gagagccaag ttccatcgtc aatgtgcctg gagagtcaac tctgcgacgt gatttcctga 2101 gactgcagca ggagaacaag gagcggtctg aggctctgcg gagacagcag cttctgcagg 2161 agcagcagct ccgggagcag gaggagtata agaggcagct gctggctgag aggcagaagc 2221 ggattgaaca gcagaaagaa cagaggaggc ggctggaaga gcaacaaaga agagaacggg 2281 aagccaggag gcagcaggag cgtgagcagc ggcggcgtga acaagaggag aagaggcgtc 2341 tcgaggaact ggaaaggcgg cgtaaagaag aggaagagag gagacgggca gaagaggaga 2401 agaggagagt ggagagggaa caggagtaca tcaggcggca gctagaggag gagcagcggc 2461 acctggagat cctgcagcag cagctgctcc aggagcaggc catgttactg cacgaccaca 2521 ggaggccgca cgcacagcag cagccgccgc ccccgcagca gcaggacagg agcaaaccga 2581 gctttcatgc tccagagccc aagcctcact atgaccctgc tgacagagct cgggaggtac 2641 agtggtccca cctggcatct ctcaagaaca atgtctcccc tgtctcgaga tcccattcct 2701 tcagtgaccc ttctcccaaa ttcgcacacc accatctccg ctctcaggac ccatgtccac 2761 cttcccgcag tgaggggctc agtcagagct ctgactctaa gtcggaggtg cccgagccca 2821 cccaaaaggc ttggtctaga tcagacagtg acgaggtgcc tccaagggtt cccgtgagaa 2881 cgacctctcg ttcccctgtc ctctcccgtc gggattcccc actgcagggc ggtggacagc 2941 agaatagcca agcaggacag agaaattcca ccagcagtat tgagccccgg cttctttggg 3001 agagagtgga aaagctggtc cccaggccag gcagtggcag ctcttcaggg tccagcaact 3061 caggatccca gcctggctcc catcctgggt ctcagagcgg ctccggtgaa cgcttcagag 3121 tgagatcatc atccaagtct gaaggttctc catcgccacg ccaggaaagt gcagccaaaa 3181 agcctgacga taagaaagaa gtattccggc ctctcaagcc tgcgggagaa gtggacttga 3241 ctgcgttggc caaagagctt cgagcagtgg aagatgttcg gccacctcac aaagtaacag 3301 actactcatc ctccagtgag gagtctggga ccacagatga ggaggaggag gacgtggaac 3361 aggagggggc tgatgattct acctcgggac cagaagacac cagagcagcg tcatctccga 3421 acctgagcaa cggtgaaaca gaatctgtga aaacaatgat cgttcatgat gatgtagaaa 3481 gtgagccagc catgaccccg tccaaggagg gcaccctcat cgtccgccag actcagtccg 3541 ctagtagcac actccagaaa cacaaatctt cctcctcctt tacacctttt atagacccca 3601 ggttactaca gatctctcca tctagtggga cgacagtgac ttccgtggtg ggattttcct 3661 gtgatgggct gagaccagaa gccataaggc aagatcctac tcggaaaggc tcagtggtca 3721 atgtgaaccc cacgaacact aggccacaga gtgatacccc cgagattcgt aaatacaaga 3781 agagatttaa ctcagagatc ctgtgtgctg ccttatgggg agtgaacttg ctggttggta 3841 cagagagcgg tctgatgctc ttggacagaa gtggccaagg gaaagtatac cctctgatca 3901 gccgaagacg gttccagcaa atggatgtgc tcgaaggcct aaatgtcctg gtgacaatat 3961 ctggcaaaaa ggataagtta cgtgtctact atttatcctg gctaagaaat aagattcttc 4021 ataatgatcc agaggttgag aagaagcagg ggtggaccac tgtgggcgac ttggaagggt 4081 gcgtgcacta taaagtcgta aaatatgaaa gaatcaagtt tctggtaatt gctttgaaga 4141 gttctgtgga agtctatgca tgggcaccga agccatatca caaatttatg gcctttaagt 4201 catttggaga actgctacat aagccattac tggtggatct cactgtggag gaaggccaga 4261 ggttgaaagt gatctatgga tcctgtgcgg gattccatgc tgttgatgtg gattcaggct 4321 cagtctatga catttatcta ccaacacata ttcagtgtag catcaaaccc catgcaatca 4381 ttatcctccc caacacggat gggatggagc tgctggtgtg ctacgaagac gagggggttt 4441 acgttaacac gtacggaagg atcaccaagg acgtggttct gcagtgggga gagatgccga 4501 catctgtagc atatatccgg tcgaatcaga caatgggctg gggagagaag gcaatagaga 4561 tacgatctgt ggaaactggt cacttggatg gtgtatttat gcacaaaagg gctcagagac 4621 taaagttcct gtgtgaacgc aatgacaagg tgttctttgc ctccgtccgc tctggtggca 4681 gcagccaggt ctatttcatg accttaggca ggacttctct tctgagctgg acgcgtacgc 4741 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 4801 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 4861 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 4921 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 4981 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 5041 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 5101 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 5161 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 5221 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 5281 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 5341 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 5401 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 5461 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 5521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 5581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 5641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 5701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 5761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 5821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 5881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 5941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 6361 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 6421 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 6481 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 6541 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 6601 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 6661 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 6721 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 6781 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 6841 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 6901 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 6961 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 7021 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 7081 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 7141 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 7201 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 7261 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 7321 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 7381 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 7441 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 7501 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 7561 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 7621 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 7681 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 7741 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 7801 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 7861 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 7921 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 7981 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 8041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 8101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 8161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 8221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 8281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 8341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 8401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 8461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 8521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 8581 ttt //