LOCUS       V031003                 5808 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031003
VERSION     V031003
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1955
                     /label="Mnat1(NM_008612)"
                     /note="Mnat1(NM_008612)"
                     /gene="Mnat1"
     CDS             1974..2003
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2022..2045
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2065..2081)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2092..2714
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3780..3827
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4059..4853)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4888..5245)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5247..5351)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5378..5806
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggacgaccag ggctgcccca ggtgtaagac caccaagtac cggaaccctt
     1081 ctttgaagct gatggtgaac gtgtgcggac acactctgtg tgagagttgt gtggacttac
     1141 tgtttgtaag aggagctgga aactgtcctg agtgtgggac tccactgaga aagagcaact
     1201 tcagggtaca actctttgaa gatcccactg ttgataagga ggttgaaatt cggaaaaaag
     1261 tactaaagat atacaataaa agggaagaag attttcctag tttaagagag tataatgatt
     1321 ttctggaaga agtggaggaa atcgttttca atttgaccaa caatgtggat ttggagaaca
     1381 ctaaaaagaa aatggaaata tatcaaaaag aaaacaaaga tgtcattcag aaaaacaaat
     1441 taaagctgac gcgggagcag gaggagttgg aggaagcatt ggaggtagaa cgccaagaac
     1501 atgagcagcg acggctgttc atacaaaagg aagaagagct gcagcaggct ctgaaaagga
     1561 agaacaagca ggccttttta gatgagctgg agagctccga cctccctgtt gctctgctcc
     1621 tggcgcagca taaagacaga tcgacccagc tggaaatgca gctcgagaag cccagatcca
     1681 tgaagccagt gacgttttcc acaggaatta aaatgggtca acagatttca ttagcaccaa
     1741 tccaaaagct ggaagaagct ctttatgagt accagcccct gcagatagag acctgtggcc
     1801 cacaagttcc tgagcaagag ctcttaggac ggcttgggta tttaaaccac gtccgggctg
     1861 catctccaca ggatcttgct ggcggctata cttcttctct tgcttgtcac agagcgctac
     1921 aggatgcgtt cagtgggctc ttctggcagc ccagaacgcg tacgcggccg ctcgagcaga
     1981 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     2041 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     2101 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2161 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2221 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2281 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2341 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2401 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2461 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2521 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     2581 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     2641 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     2701 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     2761 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3601 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3661 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3721 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3781 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3841 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     3901 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3961 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     4021 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4081 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4141 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4201 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4261 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4321 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4381 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4441 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     4501 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     4561 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4621 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4681 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4741 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4801 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4861 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     4921 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4981 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5041 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5101 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5161 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5221 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     5281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5401 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5461 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5521 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5581 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5641 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5701 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5761 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
