LOCUS       V031045                 5769 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031045
VERSION     V031045
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1916
                     /label="Myd88(NM_010851)"
                     /note="Myd88(NM_010851)"
                     /gene="Myd88"
     CDS             1935..1964
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1983..2006
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2026..2042)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2053..2675
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2824..3412)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3741..3788
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4020..4814)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4849..5206)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5208..5312)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5339..5767
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtctgcggga gacccccgcg tgggatccgg gtccctggac tccttcatgt
     1081 tctccatacc cttggtcgcg cttaacgtgg gagtgaggcg ccgcctatcg ctgttcttga
     1141 accctcggac gcccgtggcg gccgactgga ccttgctggc ggaggagatg ggcttcgagt
     1201 acttggagat ccgagagctg gaaacgcgcc ctgaccccac tcgcagtttg ttggatgcct
     1261 ggcaggggcg ctctggcgcg tctgtcggca ggctgctaga gctgctggcc ttgttagacc
     1321 gtgaggatat actgaaggag ctgaagtcgc gcatcgagga ggactgccag aaatacttag
     1381 gtaagcagca gaaccaggag tccgagaagc ctttacaggt ggccagagtg gaaagcagtg
     1441 tcccacaaac aaaggaactg ggaggcatca ccacccttga tgacccccta ggacaaacgc
     1501 cggaactttt cgatgccttt atctgctact gccccaacga tatcgagttt gtgcaggaga
     1561 tgatccggca actagaacag acagactatc ggcttaagtt gtgtgtgtcc gaccgtgacg
     1621 tcctgccggg cacctgtgtc tggtccattg ccagcgagct aattgagaaa aggtgtcgcc
     1681 gcatggtggt ggttgtttct gacgattatc tacagagcaa ggaatgtgac ttccagacca
     1741 agtttgcact cagcctgtct ccaggtgtcc aacagaagcg actgattcct attaaataca
     1801 aggcgatgaa gaaggacttt cccagtatcc tgcggttcat cactatatgc gactatacca
     1861 acccttgcac caagtcctgg ttctggaccc gccttgccaa ggctttgtcc ctgcccacgc
     1921 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc
     1981 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc
     2041 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     2101 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     2161 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     2221 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     2281 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     2341 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     2401 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     2461 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     2521 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     2581 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     2641 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     2701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3241 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3541 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     3601 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     3661 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     3721 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     3781 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     3841 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     3901 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     3961 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     4021 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4081 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4141 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     4201 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4261 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4321 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4381 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4441 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4501 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4561 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4621 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     4681 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     4741 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     4801 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     4861 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     4921 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     4981 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     5041 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     5101 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5161 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     5221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5761 gcgaatttt
//
