LOCUS       V030551                 7410 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030551
VERSION     V030551
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3557
                     /label="Nlgn1(NM_138666)"
                     /note="Nlgn1(NM_138666)"
                     /gene="Nlgn1"
     CDS             3576..3605
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3624..3647
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3667..3683)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3694..4316
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4465..5053)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5382..5429
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5661..6455)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6490..6847)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6849..6953)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6980..7408
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcacttccc agatgcatgt ggccaaatta tgtttggaga gctatgatgg
     1081 catgtgtggt ccacagggga tccggtgccc cattgactct ctgcttgttg ggatgtttgc
     1141 tacagacttt tcacgtactc tctcaaaagt tggatgatgt agacccattg gttactacta
     1201 actttggcaa gattagggga attaagaaag aactcaataa tgaaattttg ggtcctgtca
     1261 ttcagtttct tggggttcca tatgccgctc caccaacagg agaacatcgt ttccagcctc
     1321 cagaaccacc atctccctgg tctgacatcc ggaacgccac tcagtttgct cctgtatgtc
     1381 cccagaatat cattgatggc agattgcctg aagttatgct tcctgtgtgg ttcactaata
     1441 acttggatgt ggtttcatca tacgtccaag accagagtga agactgtcta tacttaaaca
     1501 tctatgtccc aactgaagat ggtcccctta caaagaaaca cacagatgat ttaggtgata
     1561 atgacggtgc tgaagatgaa gatattcggg acagtggggg tcccaaacca gtgatggtgt
     1621 acatccatgg cggctcttac atggaaggta ctggaaatct gtatgatggg agtgtcttgg
     1681 caagctatgg caatgtgatc gtcatcacag tcaactatcg gcttggggta cttggcttct
     1741 tgagcacagg ggatcaggct gccaaaggaa actacgggct ccttgacctc atccaggccc
     1801 taagatggac cagcgagaac attgggttct ttggtggtga ccccttgcga atcactgtgt
     1861 ttggatcagg cgctgggggt tcatgtgtca acctgctgac tttatcccat tattctgaag
     1921 gtaaccgttg gagcaattca accaaaggac tttttcaacg agcaatagct cagagtggaa
     1981 cagccctttc cagctgggct gttagtttcc agcctgcaaa atacgctaga attctggcca
     2041 caaaagttgg ctgcaatgtt tcagatacag tagagttagt agaatgcctg cagaagaagc
     2101 cttacaaaga acttgttgat caagatgttc aaccagcccg ataccacata gcctttggac
     2161 ctgtgatcga tggtgatgta ataccagatg accctcagat actgatggaa caaggagagt
     2221 tcctcaacta tgatataatg ttgggagtta accaagggga agggttgaag tttgtcgaaa
     2281 atatagtaga tagtgatgat ggtgtatcag ccagtgattt cgactttgct gtttctaatt
     2341 ttgttgataa tttatatgga tatcctgaag gcaaagatgt tttgagagaa accattaaat
     2401 tcatgtatac tgactgggct gatcgccata atcctgaaac tagaaggaag acattgttgg
     2461 ctttgtttac ggaccatcaa tgggtagcac ctgctgtggc cacagcagac cttcactcga
     2521 actttggctc acctacatac ttctatgcct tttatcatca ttgccaaaca gatcaagttc
     2581 cagcttgggc tgatgcagct catggggatg aggttcccta tgtgttggga atccccatga
     2641 ttggccctac agagttattt ccttgcaatt tctccaagaa tgatgtgatg ttgagtgcag
     2701 tagtaatgac atactggacg aattttgcta aaactggtga cccaaatcaa ccagttcctc
     2761 aagacacaaa attcatccat accaaaccca accgctttga agaagtagca tggaccagat
     2821 attcccagaa agaccagctt tatctccata ttggattaaa accgagagtt aaagagcatt
     2881 acagagccaa taaggtaaat ctctggctgg agctggtacc tcatctgcat aatctcaatg
     2941 acatttctca gtatacctcg acaacaacta aagtgccatc cacggacatc actctcagac
     3001 ctacaaggaa gaattccaca ccagtcacat cagcctttcc cactgccaaa caggatgatc
     3061 ccaagcaaca accaagcccc ttctcggtgg atcagaggga ctactccaca gagctaagtg
     3121 tcactatcgc agtgggggcc tctctgctgt ttctcaacat cttggctttt gcagccctgt
     3181 actacaagaa ggataagagg agacatgatg tccaccggag gtgcagccct cagcgcacga
     3241 ccaccaacga cctaacccat gctccagaag aggaaattat gtctctccaa atgaagcaca
     3301 ctgacttgga tcacgagtgt gagtccatcc atccacatga ggtggttctt cggaccgcct
     3361 gtcccccaga ttatactcta gctatgagga ggtcacctga tgatattcca ctaatgacac
     3421 ctaacaccat cacaatgatt cccaacacta taccagggat tcagccctta catacattca
     3481 acacatttac tggaggacag aataatacac tgccccatcc ccacccacac ccccattcac
     3541 attcaacaac cagggtaacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     3601 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     3661 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     3721 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3781 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3841 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3901 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3961 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     4021 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4081 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4141 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4201 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4261 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     4321 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     5221 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     5281 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     5341 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5401 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5461 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5521 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5581 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     5641 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5701 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5761 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     5821 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     5881 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     5941 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     6001 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6061 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6121 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     6181 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6241 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     6301 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6361 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6421 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6481 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6541 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     6601 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     6661 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6721 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6781 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     6841 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6961 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7321 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7381 ctgatttaac aaaaatttaa cgcgaatttt
//
