LOCUS V030967 5703 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030967 VERSION V030967 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1850 /label="Nqo1(NM_008706)" /note="Nqo1(NM_008706)" /gene="Nqo1" CDS 1869..1898 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1917..1940 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1960..1976) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1987..2609 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2758..3346) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3675..3722 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3954..4748) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4783..5140) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5142..5246) /gene="bla" /label="AmpR promoter" rep_origin 5273..5701 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggcggcgaga agagccctga ttgtactggc ccattcagag aagacatcat 1081 tcaactacgc catgaaggag gctgctgtag aggctctgaa gaagagagga tgggaggtac 1141 tcgaatctga cctctatgct atgaacttca accccatcat ttccagaaat gacatcacag 1201 gtgagctgaa ggactcgaag aactttcagt atccttccga gtcatctcta gcatataagg 1261 aaggacgcct gagcccagat attgtggccg aacacaagaa gctggaagct gcagacctgg 1321 tgatatttca gttcccattg cagtggtttg gggtgccagc cattctgaaa ggctggtttg 1381 agagagtgct cgtagcagga tttgcctaca catatgctgc catgtacgac aacggtcctt 1441 tccagaataa gaagaccttg ctttctatca ccactggggg tagcggctcc atgtactctc 1501 ttcagggtgt ccacggggac atgaacgtca ttctctggcc gattcagagt ggcatcctgc 1561 gtttctgtgg cttccaggtc ttagaacctc aactggttta cagcattggc cacactccac 1621 cagatgcccg catgcagatc ctggaaggat ggaagaaacg tctggaaacc gtctgggagg 1681 agaccccact ctattttgct ccaagcagcc tgtttgacct aaactttcag gcaggattct 1741 taatgaaaaa ggaagttcaa gaggagcaga agaagaacaa gtttggcctc tctgtgggcc 1801 atcacctggg caagtccatt ccagctgaca accagatcaa agctagaaaa acgcgtacgc 1861 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 1921 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 1981 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 2041 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 2101 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 2161 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 2221 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 2281 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 2341 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 2401 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 2461 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 2521 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 2581 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 2641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 2701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 2761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 2821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 2881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 2941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3181 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3481 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 3541 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 3601 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 3661 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 3721 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 3781 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 3841 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 3901 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 3961 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 4021 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 4081 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 4141 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 4201 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 4261 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 4321 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 4381 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 4441 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 4501 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 4561 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 4621 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 4681 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 4741 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 4801 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 4861 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 4921 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 4981 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 5041 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 5101 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 5161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 5221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 5281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 5341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 5401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 5461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 5521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 5581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 5641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5701 ttt //