LOCUS V019650 8578 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019650 VERSION V019650 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..3234 /label="OTUD7B(NM_020205)" /note="OTUD7B(NM_020205)" /gene="OTUD7B" CDS 3241..3330 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 3354..3942 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4008..4056 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4258..4686 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4700..5029 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5096..5890 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6066..6187 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6236..6252) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6260..6276 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6284..6314) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6329..6350 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6638..7226) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7397..8257) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8258..8362) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgac cctggacatg 721 gatgctgttc tgtcagattt tgtccgttcc acaggagcag agccagggct agcgcgagat 781 ctcctagaag gaaagaattg ggatgtgaat gccgccctca gtgattttga acagctacgt 841 caagtccatg ctggaaacct acccccatcc tttagtgagg ggagtggtgg ctccaggacc 901 cctgaaaaag ggttttctga cagagagcct actcgccctc cccgacccat cctccagcgg 961 caggatgaca tcgttcaaga aaaacgcctg tctaggggca tctcccacgc cagctccagc 1021 attgtttccc tggcccggtc ccatgtctcc tccaatggtg ggggtggggg gagcaatgag 1081 caccccctgg aaatgcccat ctgtgccttc cagcttccag atctcactgt atacaatgaa 1141 gacttccgca gcttcataga gagagacctc attgagcagt ccatgctggt tgccttggaa 1201 caggcagggc gtttgaactg gtgggtgagt gtggatccca cctctcagag gctgcttcct 1261 ttggcaacta ctggagatgg gaactgcctc ctgcatgcag cctcccttgg aatgtggggt 1321 ttccatgatc gggacttgat gctgcggaaa gctttgtatg cactgatgga gaagggagtt 1381 gagaaggaag cgttgaaaag gcgctggagg tggcagcaga cacagcagaa taaagagtca 1441 gggctggtat acacagaaga tgaatggcag aaggagtgga atgaactgat caagcttgcc 1501 tcaagtgaac cccgaatgca tctaggtacc aatggagcca actgtggtgg ggtggagagt 1561 tctgaggagc ctgtatatga gagccttgaa gagtttcacg tctttgtcct tgctcatgtg 1621 cttaggaggc ccatagtcgt cgtggcagac accatgctga gggactccgg aggggaagca 1681 tttgccccta ttccctttgg aggaatctat ctgcctttgg aggtcccagc cagccagtgt 1741 caccgctccc ctctggtgct cgcctatgat caggcccact tttctgcact cgtgtccatg 1801 gagcagaagg agaataccaa ggaacaagct gtgatcccac ttacagattc agagtataag 1861 ctgctgccct tgcactttgc tgtggaccct ggaaagggct gggagtgggg caaagatgat 1921 agtgacaatg tccgattggc cagtgtaatt ctgtccctag aggtcaaatt gcatctgctg 1981 catagctaca tgaatgtgaa gtggatccca ctgtcctctg atgcacaggc tcctctggcc 2041 cagcctgagt cccccaccgc ctcagctgga gatgagcccc ggtccactcc tgagtctgga 2101 gactcagaca aggagtcagt tggcagcagt tccaccagca acgagggcgg ccggcggaag 2161 gagaagtcaa agcgagatcg ggagaaggac aagaagagag cagattctgt ggctaacaaa 2221 ctgggcagct ttggcaaaac cttgggcagc aagctcaaga agaacatggg gggcctgatg 2281 cacagcaagg gttcaaagcc tggaggggtg gggacagggt tgggaggaag cagcggcact 2341 gagacactgg agaagaagaa gaaaaactca ctgaagagct ggaagggtgg caaggaggag 2401 gcagctgggg atgggcctgt gtctgagaag cccccagctg agtctgttgg taacggaggg 2461 agcaagtata gccaggaggt gatgcagagc ctgagcattc tgaggactgc catgcaaggg 2521 gaggggaagt ttatttttgt tggaaccctg aagatgggtc accgtcacca gtatcaggag 2581 gaaatgatcc agcgctacct ttctgatgct gaggagagat tcctggcaga acagaagcag 2641 aaggaggcag agaggaagat catgaatgga ggaatagggg gtggccctcc tccagccaaa 2701 aagccagagc cagatgctag ggaagagcag ccgaccggtc ccccagcaga gtccagggca 2761 atggcatttt ccactggcta ccctggggac tttactatcc ctcggccgtc tgggggcgga 2821 gtccactgcc aggaaccccg gaggcagttg gcagggggtc catgtgtcgg gggcctacca 2881 ccatatgcca ccttccccag acagtgccct cctgggcgac cctaccccca ccaggacagc 2941 atcccttctc tggagccagg cagccactct aaggatggac ttcacagggg tgccttgtta 3001 ccacccccct accgagtggc tgattcctat agcaatggct acagagagcc ccctgagcca 3061 gatggatggg ctggaggtct ccggggcctt cccccaactc agaccaaatg caaacaaccg 3121 aactgcagct tctatggaca ccctgagaca aacaacttct gttcctgttg ttacagggaa 3181 gaactgagga ggagggagcg ggaaccggat ggggagctcc tggtgcacag gttcctcgag 3241 gaacaaaaac taatatcaga ggaagatttg gaacagaaac taatcagtga agaagattta 3301 gagcaaaagt taatttccga ggaggactta tagtgagggc ccgatatctc gacaatcaac 3361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 3421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 3481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 3541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 3601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 3661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 3721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 3781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 3841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 3901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct 3961 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 4021 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 4081 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt 4141 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 4201 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg 4261 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 4321 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 4381 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 4441 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 4501 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 4561 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 4621 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 4681 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 4741 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 4861 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 4921 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 4981 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 5041 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 5101 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 5161 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 5221 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 5281 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 5341 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 5401 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 5461 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 5521 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 5581 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 5641 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 5701 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 5761 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 5821 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 5881 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc 5941 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 6001 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 6061 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6181 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 7681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 7741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 7921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 8041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 8101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 8161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 8221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 8281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 8401 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata 8461 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 8521 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg //