LOCUS       V019650                 8578 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019650
VERSION     V019650
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3234
                     /label="OTUD7B(NM_020205)"
                     /note="OTUD7B(NM_020205)"
                     /gene="OTUD7B"
     CDS             3241..3330
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3354..3942
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4008..4056
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4258..4686
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4700..5029
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5096..5890
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6066..6187
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6236..6252)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6260..6276
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6284..6314)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6329..6350
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6638..7226)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7397..8257)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8258..8362)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgac cctggacatg
      721 gatgctgttc tgtcagattt tgtccgttcc acaggagcag agccagggct agcgcgagat
      781 ctcctagaag gaaagaattg ggatgtgaat gccgccctca gtgattttga acagctacgt
      841 caagtccatg ctggaaacct acccccatcc tttagtgagg ggagtggtgg ctccaggacc
      901 cctgaaaaag ggttttctga cagagagcct actcgccctc cccgacccat cctccagcgg
      961 caggatgaca tcgttcaaga aaaacgcctg tctaggggca tctcccacgc cagctccagc
     1021 attgtttccc tggcccggtc ccatgtctcc tccaatggtg ggggtggggg gagcaatgag
     1081 caccccctgg aaatgcccat ctgtgccttc cagcttccag atctcactgt atacaatgaa
     1141 gacttccgca gcttcataga gagagacctc attgagcagt ccatgctggt tgccttggaa
     1201 caggcagggc gtttgaactg gtgggtgagt gtggatccca cctctcagag gctgcttcct
     1261 ttggcaacta ctggagatgg gaactgcctc ctgcatgcag cctcccttgg aatgtggggt
     1321 ttccatgatc gggacttgat gctgcggaaa gctttgtatg cactgatgga gaagggagtt
     1381 gagaaggaag cgttgaaaag gcgctggagg tggcagcaga cacagcagaa taaagagtca
     1441 gggctggtat acacagaaga tgaatggcag aaggagtgga atgaactgat caagcttgcc
     1501 tcaagtgaac cccgaatgca tctaggtacc aatggagcca actgtggtgg ggtggagagt
     1561 tctgaggagc ctgtatatga gagccttgaa gagtttcacg tctttgtcct tgctcatgtg
     1621 cttaggaggc ccatagtcgt cgtggcagac accatgctga gggactccgg aggggaagca
     1681 tttgccccta ttccctttgg aggaatctat ctgcctttgg aggtcccagc cagccagtgt
     1741 caccgctccc ctctggtgct cgcctatgat caggcccact tttctgcact cgtgtccatg
     1801 gagcagaagg agaataccaa ggaacaagct gtgatcccac ttacagattc agagtataag
     1861 ctgctgccct tgcactttgc tgtggaccct ggaaagggct gggagtgggg caaagatgat
     1921 agtgacaatg tccgattggc cagtgtaatt ctgtccctag aggtcaaatt gcatctgctg
     1981 catagctaca tgaatgtgaa gtggatccca ctgtcctctg atgcacaggc tcctctggcc
     2041 cagcctgagt cccccaccgc ctcagctgga gatgagcccc ggtccactcc tgagtctgga
     2101 gactcagaca aggagtcagt tggcagcagt tccaccagca acgagggcgg ccggcggaag
     2161 gagaagtcaa agcgagatcg ggagaaggac aagaagagag cagattctgt ggctaacaaa
     2221 ctgggcagct ttggcaaaac cttgggcagc aagctcaaga agaacatggg gggcctgatg
     2281 cacagcaagg gttcaaagcc tggaggggtg gggacagggt tgggaggaag cagcggcact
     2341 gagacactgg agaagaagaa gaaaaactca ctgaagagct ggaagggtgg caaggaggag
     2401 gcagctgggg atgggcctgt gtctgagaag cccccagctg agtctgttgg taacggaggg
     2461 agcaagtata gccaggaggt gatgcagagc ctgagcattc tgaggactgc catgcaaggg
     2521 gaggggaagt ttatttttgt tggaaccctg aagatgggtc accgtcacca gtatcaggag
     2581 gaaatgatcc agcgctacct ttctgatgct gaggagagat tcctggcaga acagaagcag
     2641 aaggaggcag agaggaagat catgaatgga ggaatagggg gtggccctcc tccagccaaa
     2701 aagccagagc cagatgctag ggaagagcag ccgaccggtc ccccagcaga gtccagggca
     2761 atggcatttt ccactggcta ccctggggac tttactatcc ctcggccgtc tgggggcgga
     2821 gtccactgcc aggaaccccg gaggcagttg gcagggggtc catgtgtcgg gggcctacca
     2881 ccatatgcca ccttccccag acagtgccct cctgggcgac cctaccccca ccaggacagc
     2941 atcccttctc tggagccagg cagccactct aaggatggac ttcacagggg tgccttgtta
     3001 ccacccccct accgagtggc tgattcctat agcaatggct acagagagcc ccctgagcca
     3061 gatggatggg ctggaggtct ccggggcctt cccccaactc agaccaaatg caaacaaccg
     3121 aactgcagct tctatggaca ccctgagaca aacaacttct gttcctgttg ttacagggaa
     3181 gaactgagga ggagggagcg ggaaccggat ggggagctcc tggtgcacag gttcctcgag
     3241 gaacaaaaac taatatcaga ggaagatttg gaacagaaac taatcagtga agaagattta
     3301 gagcaaaagt taatttccga ggaggactta tagtgagggc ccgatatctc gacaatcaac
     3361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct
     3961 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     4021 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     4081 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     4141 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     4201 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     4261 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4321 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4381 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4441 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4501 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4561 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4621 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4681 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4741 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4861 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4921 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4981 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5041 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5101 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5161 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5221 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5281 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5341 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5401 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5461 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5521 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5581 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5641 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5701 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5761 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5821 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5881 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     5941 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6001 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6061 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6181 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8401 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata
     8461 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     8521 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
