LOCUS       V030909                 5847 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030909
VERSION     V030909
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1994
                     /label="Or6c75(NM_146933)"
                     /note="Or6c75(NM_146933)"
                     /gene="Or6c75"
     CDS             2013..2042
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2061..2084
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2104..2120)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2131..2753
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2902..3490)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3819..3866
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4098..4892)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4927..5284)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5286..5390)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5417..5845
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcctttagaa gcagagagta ttatgagaaa ttccacagca gtgacagact
     1081 ttattcttct tggactgaca gatgatccac tgtggcagat tgtggttttc acatttcttc
     1141 ttgttaccta catgctaagt gtgactggga acctcatcat tatcatcctc accctctcag
     1201 atgcccatct gatgacaccc atgtatttct tccttcgaaa tttctcactc ctagaaatat
     1261 catttacatc tgtctgcatt cccagattcc ttgtcactat tgtgacagga gacagaacta
     1321 tttcctacaa tggttgcgtg gctcagctat tctttttcat tttcttagga gtgacagaat
     1381 tttaccttct ggctgccatg tcctatgacc gttatgtggc catctgcaaa cctctacact
     1441 acacaacaat catgagcaac cgtgtgtgca ttcttcttgt ctttagctca tggtttgcag
     1501 gattcatgat tatctttcca ccaattatcc ttctgttaca gttagacttc tgtgcctcca
     1561 acataattga ccactttatc tgtgactctt ctccaatttt gcagctttct tgctcaaaca
     1621 ctcactttct cgagctcatg gcattttcat tagctgtggt aacactcatg gtcaccttga
     1681 cattaattat tctttcctac acaaatatta ttcggacaat tctgagaatt ccttctacaa
     1741 atcagagaaa aaaggccttt tcaacttgtt cctcccatat gatagttgtc tccctctctt
     1801 atggcagctg catcttcatg tacattaagc cttctgcaag ggagagggtg actttaagca
     1861 aaggagtagc tgtactcaac acttctgtgg ctcctctcct gaaccccttc atctatacac
     1921 tgaggaacca gcaagtgaag caagccttca aaaacatgat ccagagaatc ttcttttctt
     1981 caaagaattt accaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     2041 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc
     2101 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg
     2161 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2221 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2281 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2341 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2401 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     2461 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     2521 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     2581 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     2641 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     2701 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     2761 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3301 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     3661 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     3721 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     3781 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     3841 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     3901 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     3961 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     4021 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4081 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     4141 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     4201 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc
     4261 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     4321 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     4381 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     4441 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     4501 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     4561 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     4621 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     4681 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     4741 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     4801 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     4861 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     4921 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     4981 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     5041 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     5101 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5161 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5221 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     5281 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5341 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5401 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5821 atttaacaaa aatttaacgc gaatttt
//
