LOCUS V031183 8187 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031183 VERSION V031183 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4334 /label="Pik3cg(NM_001146200)" /note="Pik3cg(NM_001146200)" /gene="Pik3cg" CDS 4353..4382 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4401..4424 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4444..4460) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4471..5093 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5242..5830) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6159..6206 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6438..7232) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7267..7624) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7626..7730) /gene="bla" /label="AmpR promoter" rep_origin 7757..8185 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggagctggag aactatgaac aaccggtggt tctaagagag gacaacctcc 1081 gccggcgccg gaggatgaag ccacgcagcg cagcaggcag cctgtcttcc atggagctca 1141 tccccattga gttcgtactg cccaccagcc agcgcatcag caagactcca gaaacagcgc 1201 tgctgcatgt ggctggccat ggcaatgtgg aacagatgaa agctcaggtg tggctgcgcg 1261 cactggagac cagtgtggct gcggagttct accaccgatt gggcccggac caattcctcc 1321 tgctctacca gaagaaagga caatggtatg agatctatga caggtaccaa gtggtgcaga 1381 ccctagactg cctgcattac tggaagttga tgcacaagag ccctggccag atccacgtgg 1441 tacagcgaca cgtaccttct gaggagacct tggctttcca gaagcagctc acctccctga 1501 ttggctatga cgtcactgac atcagcaatg tgcacgatga tgagctagag ttcactcgcc 1561 gccgtctggt tacgccccgc atggctgaag tggctggccg ggatgccaaa ctctatgcta 1621 tgcacccttg ggtaacgtcc aaacctctcc cagactacct gtcaaaaaag attgccaaca 1681 actgcatctt catcgtcatc caccgcggta ccaccagcca aaccatcaag gtctccgcag 1741 atgatactcc tggtaccatc ctccagagct tcttcaccaa gatggccaag aagaagtccc 1801 taatgaatat ctcagaaagt caaagtgagc aggattttgt attgcgggtt tgtggccgcg 1861 atgagtacct ggtgggtgaa acacccctca aaaatttcca gtgggtgagg cagtgcctca 1921 agaacggaga tgaaatacac ctggtgctcg acacgcctcc agacccagcc cttgatgagg 1981 tgaggaagga agaatggccg ctggtggatg actgcactgg agtcaccggc taccacgagc 2041 agctgaccat ccatggcaag gaccacgaga gtgtgttcac agtgtctttg tgggactgcg 2101 accgaaagtt cagggtcaag atcagaggca ttgatatccc tgtcctgcct cggaacaccg 2161 acctcactgt gtttgtggaa gcgaacatcc agcacgggca acaagtcctc tgccaaagga 2221 gaaccagccc taagcccttc gcagaagagg tactctggaa tgtgtggctg gagtttggca 2281 tcaaaatcaa agacttgccc aaaggggctc tattgaacct acagatctac tgctgcaaaa 2341 ccccatcact gtccagcaag gcttctgcag agactccagg ctccgagtcc aagggcaaag 2401 cccagcttct ctattacgtg aacttgctgt taatagacca ccgtttcctc ctccgccacg 2461 gggactatgt gctccacatg tggcagatat ctggcaaggc agaggagcag ggcagcttca 2521 atgctgacaa gctcacatcc gcaaccaatc ctgacaagga gaactcaatg tccatttcca 2581 tcctgctgga caattactgt caccccatag ctttgcctaa gcaccggccc acccctgacc 2641 cagagggaga cagggttcgg gctgaaatgc ccaatcagct tcgaaagcaa ttggaggcga 2701 tcatagccac agatccactt aaccccctca cagcagagga caaagaattg ctctggcatt 2761 ttcgatatga aagcctgaag catccgaagg cttaccctaa gctattcagc tcagtgaaat 2821 gggggcagca agaaattgtt gccaaaacgt accagctgtt agccagaagg gagatctggg 2881 atcaaagtgc tttggacgtt ggcttaacca tgcagctcct ggactgcaac ttttcagacg 2941 agaatgtccg ggccattgca gttcagaaac tggagagctt agaggacgat gacgttttac 3001 attaccttct ccagctggta caggctgtga aatttgaacc gtaccacgac agtgcgctgg 3061 ccagattcct gctgaagcgt ggcttgagga acaaaagaat cggtcacttc ttgttctggt 3121 tcctgcgaag tgagatcgca cagtccagac actatcagca gaggttcgct gtgatcctgg 3181 aggcgtacct gcgaggctgt ggcacagcca tgttgcagga cttcacacag caggtccatg 3241 tgattgagat gttacagaaa gtcaccattg atattaaatc gctctcggca gagaagtatg 3301 acgtcagttc ccaagttatt tcacagctta agcaaaagct tgaaagcctt cagaactcca 3361 atctccccga gagctttaga gttccctatg atcctggact aaaagccggt accctggtga 3421 tcgagaaatg caaagtgatg gcctccaaga agaagcccct gtggcttgag tttaagtgtg 3481 ctgatcccac agtcctatcc aacgaaacca ttggaatcat ctttaaacat ggtgatgatc 3541 tgcgccaaga catgttgatc ttgcagattc tacgcatcat ggagtccatt tgggagactg 3601 aatctctgga cctgtgcctt ctgccttacg gttgcatctc aactggtgac aaaataggaa 3661 tgatcgagat tgtaaaggat gccacaacga tcgctcaaat tcagcaaagc acagtgggta 3721 acacgggggc attcaaagat gaagtcctga atcactggct caaggaaaaa tgtcctattg 3781 aagaaaagtt tcaggccgca gtggaaaggt ttgtttactc ctgtgcaggc tactgtgtgg 3841 ccacatttgt tcttgggatc ggtgacaggc acaacgacaa cattatgatc tcagagacag 3901 gaaacctatt tcatatagac ttcggacaca ttcttgggaa ttacaagagt ttcctgggca 3961 tcaataaaga gagagtgccc ttcgtcctaa ccccagactt cttgtttgtg atgggatctt 4021 ctggaaaaaa gacaagtcca cacttccaga aattccagga tgtctgtgtt agagcttacc 4081 tagctcttcg ccatcacaca aacctgttga tcatcttgtt ctccatgatg ctgatgacag 4141 gaatgcccca gctgacaagc aaagaggaca ttgaatatat ccgggatgcc ctcaccgtgg 4201 gaaaaagcga ggaggacgct aagaaatatt tccttgatca gatcgaagtc tgcagagaca 4261 aaggatggac tgtgcagttt aactggttcc tacatcttgt tcttggcatc aaacaaggag 4321 aaaagcactc cgctacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 4381 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 4441 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 4501 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 4561 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 4621 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 4681 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 4741 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 4801 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 4861 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 4921 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 4981 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 5041 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 5101 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 6001 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 6061 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 6121 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 6181 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 6241 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 6301 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 6361 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 6421 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 6481 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 6541 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 6601 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 6661 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 6721 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 6781 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 6841 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 6901 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 6961 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 7021 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 7081 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 7141 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 7201 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 7261 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 7321 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 7381 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 7441 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 7501 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 7561 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 7621 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7681 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7741 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 7801 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 7861 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 7921 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 7981 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8041 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 8101 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 8161 atttaacaaa aatttaacgc gaatttt //