LOCUS V031224 9603 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031224 VERSION V031224 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..5750 /label="Plxnc1(NM_018797)" /note="Plxnc1(NM_018797)" /gene="Plxnc1" CDS 5769..5798 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 5817..5840 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(5860..5876) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 5887..6509 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(6658..7246) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 7575..7622 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(7854..8648) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(8683..9040) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(9042..9146) /gene="bla" /label="AmpR promoter" rep_origin 9173..9601 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggaggtctcc cggaggaaga cgccgccgcg ccccccgtac cccgccgcac 1081 cgttgcccct gatcgcctat ctgctggcgc tggcagcgcc cgcccggggc gcggacgagc 1141 cggtgtggcg atctgagcaa gccatcggag ccatcgcggc gagccgggcg gacggcgtgt 1201 tcgtggcgag cggcagctgc ctggaccagc tggactacag cctgaagaac aggctctctc 1261 gcctataccg ggaccaagcg ggcaactgca cggagcccgt ctcgttggcg ccccccgcgc 1321 ggccccggcc cgggagcagc ttcagcaagc tactgctgcc ctaccgcgag ggggcgaccg 1381 gcctcgaggg gctgctgctc accggctgga ccttcgaccg gggcgcctgc gaggtgcggc 1441 ccctgggcaa cctgaaccgc agctccctgc gcaacggcac cgaggtggtg tcatgtcacc 1501 cgcagggttc gacggctggt gtggtgtacc gcgcgagtgg caccgacctc tggtacctgg 1561 cggtggccgc cacctatgtg ctgccggagc cggagaccgc gaaccgctgc aaccccgcag 1621 catccgatcg cgacacggct atcgcgctca agaacacgga ggggcgcagt ctggccacgc 1681 aggagctggg ccgcctgaaa ctgcgcggta gtgcgggcag cctgcacttc gtggacgcct 1741 ttctctggaa cggcagtgtc tacttcccct actatcccta caactacacg agtggcgctg 1801 ccaccggctg gcccagcatg gcgcgcatcg cgcagagcac agaggtgctg ttccagggcc 1861 aggctgccct cgactgcgac cacggccacc ctgagggccg ccgcctactg ctttcctcca 1921 gcctggtgga ggctgtggac atctgggccg gcgtgttcag cgcggccact ggagagggcc 1981 aggagaggcg ctcccccgcc accacggctc tctgcctctt caggatgagt gagatccagg 2041 cgcacgccag gagctgcagc tgggactttc aagcgactga gcacaactgc aaagaagggg 2101 atcggcctga acgggtccag ccaattgcat cgtctacact gatccactct gacctgacgt 2161 ctgtttatgg caccgtggtg atgaacagga ctgttctgtt cttggggacc ggagatggcc 2221 agttactgaa ggttgttctt ggtgagaatt tgacttcaaa ctgtccagag gttatttatg 2281 aaattaaaga agaaacgcct gtgttctaca agctcgttcc tcatcccatg aagaacatct 2341 acatctacct aacagctggg aaggaggtga gaagaattcc tgttgcaaac tgcagtaaac 2401 ggaaatcctg ttcagagtgc ttagcagcgg cagaccccca ctgtggctgg tgcctcccgt 2461 tacaaaggtg cacttttcaa ggggattgca cacatgctgg gagctttgaa aactggctgg 2521 atatttcatc aggacctaaa aaatgcccga aaattcagat acttcgaagc ctccgagaga 2581 gaaccacagt gaccattgtg ggaagcatct ccgcaagaca ctcggagtgc gtggtgaaga 2641 acgcagacac gggcaagctg ctctgccagg ggagaagcca actgaactgg acctgtgcct 2701 gcaatatccc cagcagaccc agctataatg tcttagttgt caatgcgacc ttctcctttc 2761 cgtcttggaa cttatcagaa agattcaact tcaccaactg tgcgtcatta aaagaatgcc 2821 cggcgtgtat cagaagcggc tgtgcttggt gtaaacggga caagaaatgc atccacccct 2881 tcaccccctg cgaaccatca gactatgaga ggaatcagga gctctgtcaa gttgctgtgg 2941 agaagtcgcc caaggactca ggaggaggaa gagtcaagga gagcaagaga aacagaactg 3001 acggggctgt gcaggtcttc tacattaagg ccattgagcc acaaaaaata tcaaccttag 3061 ggaaaagcaa tgtgattgtt actggagcaa acttcaccca ggcgtcaaat atcaccatga 3121 tcctgcgagg aaccagtacc tgcgagaggg atgtgatccg ggttagccat gtgctaaatg 3181 acacgcatat gaaattctca cttccatcaa gccgcaaaga aatgaaagac gtttgcatcc 3241 agtttgacgg tgggacctgc tcgtctgcag gggctctgtc ctacattgct ctgccacatt 3301 gttccctgat agttcctgcc accacctgga tcagcggtgg gcaaaacata accatcatgg 3361 gcagaaattt tgacgtcatc gacaacctca tcatttcaca cgagctgaag ggaaacgcaa 3421 atgtgaacat aaatgtctct gaatactgcg cagcaacttt ctgcaggttt ctagctccta 3481 atttaaagag ctccaaagta cgtacaaacg tggccgtgaa gctgagggtc caagacacct 3541 acttggactg tggaacgctg caatacctcg aggaccccag atttacaggg taccgagtgg 3601 agtcagagat tgatacagaa ttggaagtaa aaattcaaaa agaaaatgac aatttcaaca 3661 tttccaagga tgacattgat attactctct tccatgggga aaataaacaa tttaattgca 3721 gttttgaaaa tattactaga aaccaggatc tcacaaccat cctttgcaag atcaagagta 3781 tcaagaatgc aaacaccatc gctagctcct ctaaaaaagt ccgcgtcaag ctgggaaacc 3841 tggaactcta tgtggaacag gaatcagttc cttccacgtg gtattttcta atcgcgcttc 3901 caatcttgct ggccattgtc atcgttgtgg ctgtggtggt gacgaggtac aaatccaagg 3961 agctcagtcg aaagcagagc caacagctgg agctgctgga gagcgaactc cggaaggaga 4021 tccgcgacgg ctttgccgag ctccagatgg ataagttgga tgtggttgat agctttggaa 4081 cagtaccctt ccttgactac aaacatttcg ctctgagaac tttcttccct gagtcaggtg 4141 gcttcacgca catcttcact gaagatatgc acaacagaga cgccaatgac aagaacgaga 4201 gcctcacagc cttggatgcc ctcatatgta ataaaagctt cctggttacc gtcatccata 4261 ccctggagaa gcagaagaac ttctcagtaa aggacaggtg tctcttcgcc tccttcctaa 4321 ccattgctct gcaaaccaaa ctggtctacc tgaccagcat cctggaggtg ctgaccaggg 4381 acctgatgga gcagtgcagt aatatgcaac caaagctgat gctgaggcgc acggagtctg 4441 tggtggagaa gctcctgacg aactggatgt ctgtctgtct ctccggtttc ctccgggaga 4501 ctgttggaga gccattctat ttgctggtga cgaccctgaa ccagaaaata aacaagggtc 4561 ccgtggacgt gatcacctgc aaagcgctgt acaccctgaa cgaagactgg ctactctggc 4621 aggtccccga attcaacact gtggcattaa atgtcgtctt tgaaaaaatc ccggaaaacg 4681 agagtgcaga tgtctgccgg aatatttcag tcaacgttct tgactgcgat accataggcc 4741 aagccaaaga aaaggttttc caagccttcc taagcaaaaa tggttctcca tacggactcc 4801 agctcaatga gattggtctt gagcttcagg tgggaacacg gcagaaagaa ctcctggaca 4861 ttgacagttc ctctgtgatt cttgaagatg gaataaccaa gctaaacacc attggccact 4921 atgagatatc aaatggatcc actataaaag tctttaagaa gatagcaaat tttacttcag 4981 atgtggagta ctcagatgac cactgccact tgattctgcc agattcagaa gcattccaag 5041 tagttcaagg gaagagacat cgagggaaac acaagttcaa agtaaaagaa atgtatctga 5101 ccaagctgct gtcgaccaag gtggcaattc actctgtgct tgagaaactc ttccgaagca 5161 tttggagttt gcccaatagc agggccccat ttgctataaa atacttcttt gactttctgg 5221 atgcccaagc tgaaaacaaa aaaatcacag accctgatgt tgtacacatc tggaaaacaa 5281 acagcctccc tcttcgattc tgggtaaaca tcctgaagaa ccctcagttt gtctttgaca 5341 ttaagaagac accacacata gacagctgtt tgtcagtgat tgcccaagca ttcatggatg 5401 ccttttctct cacagaacag caactgggga aggaagcacc aaccaataaa cttctctatg 5461 ccaaggatat accaacctac aaagaggaag tgaagtctta ctacaaagca atcagggatt 5521 tgcctccatt gtcatcttta gaaatggaag aattcttaac tcaggaatct aagaaacatg 5581 aaaatgaatt taatgaagaa gtggccttga cagaaatcta caaatacatc gtaaagtatt 5641 ttgatgagat tctaaataaa ctagaaagag aacgagggct ggaagaagct cagaaacaac 5701 tcttgcatgt aaaagtctta tttgatgaaa agaagaaatg caagtggatg acgcgtacgc 5761 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 5821 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 5881 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 5941 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 6001 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 6061 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 6121 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 6181 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 6241 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 6301 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 6361 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 6421 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 6481 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 6541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7381 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 7441 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 7501 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 7561 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 7621 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 7681 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 7741 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 7801 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 7861 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 7921 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 7981 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 8041 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 8101 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 8161 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 8221 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 8281 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 8341 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 8401 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 8461 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 8521 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 8581 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 8641 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 8701 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 8761 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 8821 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 8881 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 8941 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 9001 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 9061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 9121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 9181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 9241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 9301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 9361 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 9421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 9481 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 9541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 9601 ttt //