LOCUS       V020937                 8954 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020937
VERSION     V020937
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             671..3610
                     /label="Ppfibp1(NM_001170433)"
                     /note="Ppfibp1(NM_001170433)"
                     /gene="Ppfibp1"
     CDS             3617..3706
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3730..4318
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4384..4432
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4634..5062
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5076..5405
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5472..6266
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6442..6563
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6612..6628)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6636..6652
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6660..6690)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6705..6726
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7014..7602)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7773..8633)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8634..8738)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgccacc atgatgagtg acgctagtga catgctggcc gcggcgctgg agcagatgga
      721 cggaatcata gcaggttcta aagccttgga gtattccaat gggatttttg attgccaatc
      781 tcccacctcc ccatttatgg gaagcttgcg cgctctgcac cttgtggaag acctgcgtgg
      841 attgttggag atgatggaaa cggatgagaa ggaaggattg agatgccaga tcccagattc
      901 aacagcagaa gtgcttattg agtggcttca aaatcaaatg acaaatggac atctacctgg
      961 gaatggagat gtgtatcaag aaaggttggc acgtctagaa aacgacaagg aatccctcgt
     1021 tcttcaggta agtgtgttga cagaccaggt ggaggctcag ggagagaaga ttcgggattt
     1081 ggagttctgt cttgaagagc acagagagaa gctgaatgcc actgaagaaa tgctgcagca
     1141 ggagcttctg agtaggacct ccttagagac acagaagctg gagctgatgg ctgagatctc
     1201 taacttgaag ttaaaactga cggccgtaga gaaggacagg ctggattatg aagatcggtt
     1261 cagagacaca gaggggctaa ttcaagagat caatgatttg aggctaaaag tcaatgaaat
     1321 ggacggtgaa agacttcagt atgaaaagaa gctcaagtca actaaagacg aattggcatc
     1381 tttaaaagag caactggagg agaaggaatg tgaagtaaag aggctgcagg agagactggt
     1441 ttgtaaggcg aaaggagaag ggatcgaagt cctcgatcga gatgaaaata ttaaaaagaa
     1501 gctcaaagag aaaaacattg aagtacagaa aatgaagaag gcagtggagt ctctgatggc
     1561 agcgaatgaa gaaaaggagc ggaaaataga agacctgcgg cagtgcctga gcaggtacag
     1621 gaaaatgcag gacccagcag ttctggccca aggtcaggat agtgagtgtg agggcctgtt
     1681 ccattccagc tctatctcca ccctgttgga tgcacagggt tttagtgatc tggagagaag
     1741 cacttcttct acgccaggaa tggggtctcc cagcagggac ctgctccaca cgagtgctcc
     1801 agaagagttt cacaccagcg tcttgcaagc ttcaatccct tcactattgc cgccatctgt
     1861 ggatgtagac acttgtgaaa aacccaagtt gcctaccaag cccgagactt catttgaaga
     1921 gggtgacgga agagcaatcc tgggcgctgc cgctgaagtc tccctgagtg atggtgtttc
     1981 aacttcaagt ctgcagaaat ctagcagcct tggaaatctg aagaaagagg catcggatgg
     2041 cacggataaa gctccgactg acagccgcac gtttgggacc ctgcctccca aggtcccagg
     2101 acacgaagcc tctgtggatg acaacccctt tggcactcgc aaagccaggt cctcatttgg
     2161 ccggggcttt tttaaaatca aaagtggaaa gaggacagca agtgcaccca accttgctga
     2221 gacagaaaaa gagacagctg agcacctgaa tttggctggt acatctcggt caaaaggttc
     2281 ccagggaacc agtccattcc caatgtctcc accatctcca gattccagaa agaaatccag
     2341 aggcatcatg agactctttg ggaaacttag aagaagtcag tcaactacat tcaacccaga
     2401 tgacatgtct gagcctgaat tcaaaagagg agggacaagg gcaactgcag gacccaggct
     2461 tggttggtct cgagatttag gacagtccaa cagtgacctg gatatgccat ttgccaaatg
     2521 gaccaaggag caggtgtgta gctggctggc agagcaaggc ttggggtcct acctgagttc
     2581 tggcaagcac tggattatat ctggccagac ccttttgcag gcttctcagc aggatctgga
     2641 gaaggaactt ggcatcaagc attcactgca tcgtaagaag ctccagctgg ccctgcaggc
     2701 cctgggatct gaagaagaaa ccaactatgg gaagctagac ttcaactggg ttactagatg
     2761 gctggatgat attggcctcc ctcagtacaa gacccagttt gatgaagggc gggtggacgg
     2821 tcgaatgctg cactacatga ctgtggatga cctactgtcc ctgaaggttg tcagtgtgct
     2881 gcaccatctc agtatcaaaa gggccatcca agtgctgagg atcaataact ttgagccaaa
     2941 ctgtctaagg aggcggccgt ctgatgagaa tagcatcacc ccatctgagg ttcagcagtg
     3001 gaccaatcat cgtgtgatgg agtggcttcg ctctgtggac ttggcagaat atgcccccaa
     3061 tctcagaggc agtggtgtcc atggagggct catggttctt gagcctcgtt ttaatgtaga
     3121 aactatggcc cagttactga acattccacc aaataaaacc ttgttgcgca gacatttggc
     3181 gacccatttc aaccttctga ttggtgcaga ggcccagcac cagaagcgag atgccatgga
     3241 gttaccggat tatgtactgc taacagccac tgccaaagtg aagccgaaga agctgacctt
     3301 tagcaacttt gggaatctga ggaagaagaa acatgaagat ggagaggaat atgtttgtcc
     3361 gatggagcta ggccaggcat caggtagctc ccagaagggg tttagacctg gcttggacat
     3421 gcgcctgtat gaagaggatg atttggatcg gttagagcag atggaagatt cagaagggac
     3481 ggtgagacag ataggtgcat tttctgaagg catcaacaat ctcacgcaca tgttaaagga
     3541 agatgacatg tttaaggatt ttgctgcccg ctccccgagt gccagcatca cagatgaaga
     3601 ctccaacgtt ctcgaggaac aaaaactaat atcagaggaa gatttggaac agaaactaat
     3661 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagt gagggcccga
     3721 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4201 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4261 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4321 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     4381 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     4441 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     4501 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     4561 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct
     4621 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4681 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4741 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     4801 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4861 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4921 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4981 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     5041 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     5101 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5161 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5221 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5281 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5341 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5401 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5461 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5521 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5581 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5641 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5701 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5761 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5821 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5881 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5941 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     6001 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6061 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6121 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6181 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6241 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     6301 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6361 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6421 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6541 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7201 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7441 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8521 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8581 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8761 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct
     8821 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8881 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8941 agaatctgct tagg
//
