LOCUS       V045747                11337 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045747
VERSION     V045747
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6017
                     /label="Ppl(NM_008909)"
                     /note="Ppl(NM_008909)"
                     /gene="Ppl"
     CDS             6024..6089
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6113..6701
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6767..6815
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7017..7445
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7459..7788
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7855..8649
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8825..8946
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8995..9011)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9019..9035
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9043..9073)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9088..9109
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9397..9985)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10156..11016)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11017..11121)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgca ctcgctcttc aggaagagaa
      781 acaaaggcaa atacagccca acggtgcaga cgcggagcat ctccaacaag gaactctcag
      841 atctgattga gcagctgcag aagaatgcag accaggtgga aaggaacatt gtggacaccg
      901 aggccaagat gcagagcgac ttggcccgga tgcaagaggg gcagctccct gagcaccggg
      961 acgcggccct gcagaacgtg tctgactcag aaaagctgct gtacgtgctg gaggcagatt
     1021 cggccatcgc caagcacatg aagcacccac agggggacat gatcgccgag gacatccgcc
     1081 agctgaaaga gcgcgtgacc aacctgcggg ggaaacacaa gcagatgtac agcctggcag
     1141 tgaaagaggc cgacccgaga gtcaactggg acacactggt ggacgagaag ctggacaagc
     1201 tgagcagcca gagctttggg actgaccttc ctctggtgga tagccaggtg gagcaacaca
     1261 acatcttcca caatgaagtc aaagccatcg ggccccacct ggccaaggac aaggagcaga
     1321 acagtgaact ccaagccaag tatcagaagt tgctgacagc atcacaggca cggcagcagc
     1381 acctgagctc gctgcaggat tacatgcagc gctgcaccaa tgagctgtac tggctggacc
     1441 agcaagccaa gggccgcatg cagtatgact ggagcgaccg caacctcgac taccccagcc
     1501 gccgccgcca gtacgagaat ttcatcaacc ggaacctgga ggccaaagaa gaaagaatca
     1561 acaaactgca cactgaaggt gaccaactgc tgacagctga gcacccaggg aggaactcca
     1621 ttgaggcaca catggaggct gtgcatgctg agtggaagga gtacctgaac ctgctcatct
     1681 gtgaggagag ccatctgaaa tacatggaag actaccacca atttcacaaa gacatgaagg
     1741 atgctcagga gctgctacgc aaggtggact cagacttgaa ccagaaatat agccctgact
     1801 tcaaagaccg gtatcagata gagctgttgc ttcgggaact ggatgaccag gagaaggctc
     1861 tggacaagta tgaggatgtg gtgcgagggc tgcagaggcg agggcagcaa gtggtgccac
     1921 tcaagtaccg ccgcgagacg ccgctcaagc ccatccctgt ggaggctctc tgtgactttg
     1981 agagtgacca gggcctcatc tcgcggggct atagctacac actgcagaag aacaacgggg
     2041 agagctggga gctgacagac agcactggga agaagctggc tgccccagcc gtctgcttca
     2101 tcattccccc caccgacccc gaggccttgg ctcttgctga cagcctgggc agccagtacc
     2161 gaagtgtgag gcagaaggcg actgggagca aacatgcact ccagcagcgg catgaggtgc
     2221 taaggacgga gaaccctgga gatgcctctg atcttcaggg acgacagctg ctggctggct
     2281 tggacaaagt ggccagtgac ctggaccggc aggaaaaggc tatcacaggg atcctacggc
     2341 caccacttga gcagggacgg gcaattgagg atagtgcgga gcgggccaag ggtctcaaga
     2401 acattaccaa tgagctgcta cagattgagc cagagaagac gcagtgcaca gccgagtgtg
     2461 aagcctttgt gcaggccctc ccggccagcg gcaccgcacc cttgctgaag acccgggtag
     2521 aagacaccaa ccagaaatat gagcgactag tatggttgct agaggcagcc caggagaagg
     2581 tggacgtggc caaccgcctg gagaacagtc tgcagcgggg cagggagctg ctggcctcat
     2641 acgagaacag gctgattcag gacgacacaa tgcctgaaag tggtcatgtg ttggacagca
     2701 agaggcagga gctggaggcc atggcttctg agctgcaggc ccacaagtcc ctcctgggtg
     2761 aggtggaaca gaacctacag gtggccaagc agtgttccag ctcactggcc agccgcttcc
     2821 aagagcactg ccctgacctg gagcggcagg aggccgaggt gcacaagctc aaccaacgtt
     2881 tcaacaacct cagccagcag gtggaacgga gggcccagag cctgcagagt gccagggctg
     2941 cctacgatga atactgcagt ggttacaacc gtgtgctcca gtttttggcc aagactccca
     3001 gttatgagcc ccaggagaca gacagcctcg gccagatgga gaccaagctc aagaaccaga
     3061 agaacttgct tgatgagcta gcaagcaggg aacaggaggt gcagaaggtc tatgcagatt
     3121 cccagcagta ccagcaagcg gtgaaggact atgaacttga agcagagaag ctaaggtcct
     3181 tgctggacct ggagaatgga cggaacagcc atgtgaacaa gagagccagg ctgcagtctc
     3241 ctgctgccaa agtgaaggaa gaggaagctg ctcttgctgc caagttcaca gaggttaatg
     3301 ccatcaacag acagaggcta cagaatttgg agtttgcact gaatctcttg agacagcaac
     3361 cagaagcagg agtgacccat gagaccctac aagggggaaa gctaagctct ggcatggagg
     3421 aaacatggaa gattaagaag gaactggagg aggagataga gcgaaggcaa cagctggaaa
     3481 atgaggtcaa gagtgcccag gaggaaatcc agaccctgaa ggatcagggg cctcaggagt
     3541 cattggtgag gaaggaggtg ctgaagaagg tgccagaccc tgccctggag gagagcttcc
     3601 agcagctgca gcagacgctg gctgaggaac agcacaagaa ccagctattg caggaggagc
     3661 tgggagcact gcagctccgg ctgcaggctc tagagcagga gaccagggat gggggacagg
     3721 agtatgtggt caaggaggtc ttgcgcattg agccagacag agcccaggag gatgaggtct
     3781 tgcagcttcg ggaggagctg gaggcactgc ggcgccagaa aggcgcccga gaggctgaag
     3841 tgctactctt acagcagcgt gtagcagccc tggctgctga gaagagcagg gtgcaggaaa
     3901 aggtcactga gagagaggtg gtaaagctac agaatgaccc ccaactggag gcagaatacc
     3961 ggaggctgca ggaggagcac cagcgggagg gcacactcag ggagaagcag gaggaggagc
     4021 tgagtttcct acaggctaag ctcaggagac tggagaaaga acgagccatg gcagaaggca
     4081 agatcactgt caaagaggtg ctcaaggtag agaaagatgc agctgtggag agagaagtca
     4141 atgacctcac ccgccagtat gaggacgagg ctgccaaggc acgctctggc cagcgggaga
     4201 agacagaact tctcagaaag atatgggccc tggaagagga aaatgctaag gtggtggtac
     4261 aggagaaggt ccgagagatc gtgcggccag atcccaaggc agagagtgaa gtggccaacc
     4321 tccgcctgga gctggtggag caggagcgca aattcagggg tgctgaggag cagctgaaga
     4381 gctaccagag tgagctggag gccctacgga accgtggacc acaggtagaa gtcaaagagg
     4441 tgaccaaaga ggtcattaaa tatacaactg acccagagac agagcaggaa ctccagaggc
     4501 tcagggagga gatcatggac aagaccaggt taatagaaag gtgtgaccta gagatttacc
     4561 agctgaagca ggagatccag gcccttaagg acaccaagcc acaggtgcag actagagagg
     4621 tagtccagga gatcctgcag ttccaggaag acccccaaac caagaaggag gtagagtctc
     4681 tgcgtataca gctgtcagaa gaacagaaga aacaggtgga cctggaaggg gagcgggcat
     4741 cccaggaaga gaagatcaaa cgcaaggagg aagagctggc acaggggaag gaaagggttg
     4801 tacgccagga ggtagtacag tatgaagatg agccagattt gcgagctgag gtgactgcct
     4861 tcacaaacag cattgatgct gagctgcggc agattgataa actgcatgtg gagctacggc
     4921 ggttacagca tcgccgagca gagctggaga ggcagttgga ggagctggag cgggagaggc
     4981 aggcacgtag ggcagccgag ctggaggtac agaggctgca acagcggctg gctgcattgg
     5041 agcaggagga ggccaagact ggtgagaagg tgacccacac acaaaaggtg gtgctgcagc
     5101 aggacccaca gcagaccagg gagcatgccc tgctccgagc ccagctggag gaagagcggc
     5161 accggaggca gctactggag ggtgagcttg agccgctgcg tagaaagttg gctgccctgg
     5221 agaaggcaga gatcaaagaa aaggtggtct tctctgagag tgtccaggta gaaaaaggtg
     5281 acactgagca agagatccag cgcctcaaga agagcctgga agaggagagc cagagcaaga
     5341 gggagctgga ttcagaggtg acccggctag aagccaagtt atctgagcta gaattctata
     5401 attccaagtc atccaaggaa ctggacttcc tgagagaaga aaaccacaag ctacagctag
     5461 agcggcaaaa cctgcagcta gagacccgga gactccaatc agagattgag atggcagcaa
     5521 cagaaactcg agatctgaag aacataacca cgatagactc tgggacccac ctaaactcta
     5581 ggctgtggtc cctagagaag gaactggatg acctcaagaa gatgtccaag gacaaggatc
     5641 tggagatcga tgaactacag aggcgcctgg gctctgtggc tgtcaagaga gagcagaggg
     5701 agaaccacct gaggcgctcc atagtggtca tagaccctga cacaggtcgt gagctgtccc
     5761 cagaggaggc tcaccgggct gggcttatcg actggaaaat gtttgtgaag ctcagaagcc
     5821 aggagtgcga ctgggaggag atatcagtga aaggtcctaa tggggagtca tcagtaattc
     5881 atgacaggaa gtcaggcaag aagttctcca ttgaggacgc tcttcagagt ggcaggctaa
     5941 ccgctgctca atatgaccgc tatgtcaaca aggatatgtc cattcaggag ctggcagtct
     6001 tggtgtctgg gcagaaggga tccgactaca aagaccatga cggtgattat aaagatcatg
     6061 acatcgacta caaggatgac gatgacaagt agtgagggcc cgatatctcg acaatcaacc
     6121 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6181 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6241 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6301 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6361 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6421 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6481 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6541 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6601 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6661 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaaacg ggggaggcta
     6721 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     6781 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6841 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6901 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6961 ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt atccccacgc
     7021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7141 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
     7201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7381 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7441 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7801 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     7861 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7921 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7981 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     8041 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8101 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8161 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8221 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8281 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8341 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8401 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8461 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8521 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8581 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8641 ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc
     8701 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8761 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8821 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8941 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     9601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11161 gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag
    11221 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    11281 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagg
//
