LOCUS V027963 5744 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027963 VERSION V027963 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1010..1948 /label="Prkra(NM_011871)" /note="Prkra(NM_011871)" /gene="Prkra" CDS 1958..1981 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2001..2017) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2028..2650 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2799..3387) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3716..3763 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3995..4789) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4824..5181) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5183..5287) /gene="bla" /label="AmpR promoter" rep_origin 5314..5742 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgccacca tgtcccatag 1021 caggcatcgt gccgaggccc cgccgctgca gcgcgaggac agcgggacct tcagtttggg 1081 caagatgata acagctaagc ctgggaaaac accgattcag gtattgcacg agtacggcat 1141 gaagaccaag aacattccag tttatgaatg tgaaagatcc gatgtgcaag tacacgtgcc 1201 cactttcacc ttcagagtaa ccgttggtga cataacttgc acaggtgaag gtacaagtaa 1261 gaagctggcg aagcacagag ctgcggaggc cgccataaac attttgaaag ccaatgcaag 1321 tatttgcttt gcagttcctg accccttaat gcctgatcca tccaaacagc cgaagaacca 1381 gctgaatcca attggctcat tacaggaatt agcaattcac catggctggc gacttcctga 1441 atataccctt tcccaggaag gaggaccggc tcataagagg gaatacacca cgatctgcag 1501 gcttgaatca ttcatggaaa ctggaaaggg ggcatcaaaa aaacaagcca agagaaatgc 1561 tgctgagaaa tttctcgcca agtttagtaa tatttctcca gagaaccaca tttctctaac 1621 gaacgtggtt ggacattccc taggatgcac ttggcactcc ttgaggaatt cccctggtga 1681 gaagatcaac ctcctgaaaa ggagcctcct cagtctcccg aacacagact acatccagct 1741 gcttagtgag attgccagcg agcaaggctt taacataacg tatttggata tagaggagct 1801 gagtgccaac ggacagtatc agtgtctcgc agagctgtcc accagtccta tcaccgtgtg 1861 tcacggctca ggcatctcct gtggcaatgc acagagtgac gctgctcaca atgctctgca 1921 gtatttaaag ataatagcag aaagaaagga tatcctggat tacaaggatg acgacgataa 1981 ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct 2041 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc 2101 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta 2161 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct 2221 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct 2281 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 2341 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat 2401 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat 2461 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac 2521 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg 2581 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc 2641 ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa ggcggtaata 2701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3541 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca 3601 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg 3661 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca 3721 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct 3781 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc 3841 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag 3901 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc 3961 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat 4021 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag 4081 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc 4141 gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca 4201 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc 4261 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat 4321 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg 4381 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag 4441 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca 4501 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc 4561 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca 4621 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga 4681 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct 4741 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc 4801 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac 4861 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt 4921 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga 4981 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct 5041 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc 5101 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac 5161 agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg 5221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5281 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg 5341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 5401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 5461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 5521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 5581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 5641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 5701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt //