LOCUS       V027963                 5744 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027963
VERSION     V027963
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1010..1948
                     /label="Prkra(NM_011871)"
                     /note="Prkra(NM_011871)"
                     /gene="Prkra"
     CDS             1958..1981
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2001..2017)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2028..2650
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2799..3387)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3716..3763
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3995..4789)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4824..5181)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5183..5287)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5314..5742
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgccacca tgtcccatag
     1021 caggcatcgt gccgaggccc cgccgctgca gcgcgaggac agcgggacct tcagtttggg
     1081 caagatgata acagctaagc ctgggaaaac accgattcag gtattgcacg agtacggcat
     1141 gaagaccaag aacattccag tttatgaatg tgaaagatcc gatgtgcaag tacacgtgcc
     1201 cactttcacc ttcagagtaa ccgttggtga cataacttgc acaggtgaag gtacaagtaa
     1261 gaagctggcg aagcacagag ctgcggaggc cgccataaac attttgaaag ccaatgcaag
     1321 tatttgcttt gcagttcctg accccttaat gcctgatcca tccaaacagc cgaagaacca
     1381 gctgaatcca attggctcat tacaggaatt agcaattcac catggctggc gacttcctga
     1441 atataccctt tcccaggaag gaggaccggc tcataagagg gaatacacca cgatctgcag
     1501 gcttgaatca ttcatggaaa ctggaaaggg ggcatcaaaa aaacaagcca agagaaatgc
     1561 tgctgagaaa tttctcgcca agtttagtaa tatttctcca gagaaccaca tttctctaac
     1621 gaacgtggtt ggacattccc taggatgcac ttggcactcc ttgaggaatt cccctggtga
     1681 gaagatcaac ctcctgaaaa ggagcctcct cagtctcccg aacacagact acatccagct
     1741 gcttagtgag attgccagcg agcaaggctt taacataacg tatttggata tagaggagct
     1801 gagtgccaac ggacagtatc agtgtctcgc agagctgtcc accagtccta tcaccgtgtg
     1861 tcacggctca ggcatctcct gtggcaatgc acagagtgac gctgctcaca atgctctgca
     1921 gtatttaaag ataatagcag aaagaaagga tatcctggat tacaaggatg acgacgataa
     1981 ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct
     2041 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2101 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2161 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     2221 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     2281 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     2341 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     2401 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     2461 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac
     2521 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg
     2581 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc
     2641 ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa ggcggtaata
     2701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3541 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     3601 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     3661 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     3721 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     3781 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3841 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3901 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3961 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     4021 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     4081 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     4141 gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     4201 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     4261 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     4321 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     4381 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     4441 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     4501 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     4561 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     4621 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     4681 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     4741 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     4801 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     4861 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     4921 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     4981 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5041 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     5101 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     5161 agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg
     5221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5281 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     5341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
