LOCUS V030871 6126 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030871 VERSION V030871 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2273 /label="Ptgir(NM_008967)" /note="Ptgir(NM_008967)" /gene="Ptgir" CDS 2292..2321 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2340..2363 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2383..2399) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2410..3032 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3181..3769) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4098..4145 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4377..5171) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5206..5563) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5565..5669) /gene="bla" /label="AmpR promoter" rep_origin 5696..6124 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gatggccagc gatggacatc ctggaccccc ttctgtcact cctgggagcc 1081 ctctcagtgc aggtggcaga gagtggcagg gcatggcagg ctcctgctgg aatatcacct 1141 acgttcaaga ctcggtagga cctgccacca gcacgctgat gttcgtggct ggtgtagtgg 1201 gcaacgggct ggcattgggc atcctgggtg cccgacggag gtcacaccca tcggcctttg 1261 cggtgttggt cactgggctg gcggtgacgg atctgctggg cacgtgcttc ttgagccctg 1321 cagtgtttgt ggcctatgct cgaaacagct ctctgctggg cctggcccac ggtgggacga 1381 tgctgtgtga cactttcgcc ttcgccatga cgttcttcgg cctggcctcc acgctcatcc 1441 tctttgccat ggctgtggag cggtgcctgg cactcagtca cccctacctg tacgcccagc 1501 tggacgggcc gcgctgcgcc cgctttgcct tgccttccat ctacgctttc tgctgcctct 1561 tctgctcact gcccctgctg ggcctgggcg agcatcagca gtattgccca gggagctggt 1621 gcttcatccg catgcgttcc gcccagcctg gcggctgtgc cttctccctg gcctacgcca 1681 gtctcatggc cctgttggtg acctccatct tcttctgcaa cggctccgtc actctcagcc 1741 tctatcacat gtaccgccaa cagagacgcc accatggctc gtttgtaccg acctctcggg 1801 cacgagagga tgaagtttac cacctgattc tgctggccct catgaccgtc atcatggccg 1861 tgtgctccct gcctctcatg atccgaggct tcactcaggc catcgcccca gacagcagag 1921 agatggggga cctcctcgcc ttccgcttca acgccttcaa ccccatcctg gacccctggg 1981 tcttcatcct tttccgaaag gctgtcttcc aacgcctcaa gttctggttg tgttgcctgt 2041 gtgcccgttc tgtccatggg gatttgcagg cgcccctttc cagacctgca tcggggagaa 2101 gagacccacc ggctcctact tctctccagg ctaaggaagg gagctgggtg cccctgtcgt 2161 cctggggcac gggacaggtg gcaccattga ctgctgtgcc tctgactggt ggtgacggct 2221 gctctgtggg aatgccatcc aagtcagagg ccatcgctgc ctgctccctc tgcacgcgta 2281 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg 2341 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga 2401 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt 2461 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac 2521 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt 2581 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca 2641 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct 2701 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg 2761 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc 2821 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct 2881 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta 2941 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc 3001 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc 3061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 3181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 3241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 3301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 3361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 3421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 3481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 3541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 3601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 3661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 3721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 3781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 3841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 3901 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt 3961 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac 4021 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga 4081 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat 4141 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc 4201 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc 4261 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc 4321 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag 4381 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg 4441 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta 4501 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca 4561 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg 4621 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc 4681 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt 4741 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta 4801 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat 4861 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg 4921 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct 4981 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg 5041 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc 5101 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct 5161 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag 5221 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc 5281 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg 5341 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg 5401 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga 5461 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca 5521 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa 5581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg 5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 6121 aatttt //