LOCUS       V030871                 6126 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030871
VERSION     V030871
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2273
                     /label="Ptgir(NM_008967)"
                     /note="Ptgir(NM_008967)"
                     /gene="Ptgir"
     CDS             2292..2321
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2340..2363
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2383..2399)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2410..3032
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3181..3769)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4098..4145
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4377..5171)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5206..5563)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5565..5669)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5696..6124
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gatggccagc gatggacatc ctggaccccc ttctgtcact cctgggagcc
     1081 ctctcagtgc aggtggcaga gagtggcagg gcatggcagg ctcctgctgg aatatcacct
     1141 acgttcaaga ctcggtagga cctgccacca gcacgctgat gttcgtggct ggtgtagtgg
     1201 gcaacgggct ggcattgggc atcctgggtg cccgacggag gtcacaccca tcggcctttg
     1261 cggtgttggt cactgggctg gcggtgacgg atctgctggg cacgtgcttc ttgagccctg
     1321 cagtgtttgt ggcctatgct cgaaacagct ctctgctggg cctggcccac ggtgggacga
     1381 tgctgtgtga cactttcgcc ttcgccatga cgttcttcgg cctggcctcc acgctcatcc
     1441 tctttgccat ggctgtggag cggtgcctgg cactcagtca cccctacctg tacgcccagc
     1501 tggacgggcc gcgctgcgcc cgctttgcct tgccttccat ctacgctttc tgctgcctct
     1561 tctgctcact gcccctgctg ggcctgggcg agcatcagca gtattgccca gggagctggt
     1621 gcttcatccg catgcgttcc gcccagcctg gcggctgtgc cttctccctg gcctacgcca
     1681 gtctcatggc cctgttggtg acctccatct tcttctgcaa cggctccgtc actctcagcc
     1741 tctatcacat gtaccgccaa cagagacgcc accatggctc gtttgtaccg acctctcggg
     1801 cacgagagga tgaagtttac cacctgattc tgctggccct catgaccgtc atcatggccg
     1861 tgtgctccct gcctctcatg atccgaggct tcactcaggc catcgcccca gacagcagag
     1921 agatggggga cctcctcgcc ttccgcttca acgccttcaa ccccatcctg gacccctggg
     1981 tcttcatcct tttccgaaag gctgtcttcc aacgcctcaa gttctggttg tgttgcctgt
     2041 gtgcccgttc tgtccatggg gatttgcagg cgcccctttc cagacctgca tcggggagaa
     2101 gagacccacc ggctcctact tctctccagg ctaaggaagg gagctgggtg cccctgtcgt
     2161 cctggggcac gggacaggtg gcaccattga ctgctgtgcc tctgactggt ggtgacggct
     2221 gctctgtggg aatgccatcc aagtcagagg ccatcgctgc ctgctccctc tgcacgcgta
     2281 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     2341 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     2401 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     2461 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     2521 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     2581 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     2641 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     2701 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     2761 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     2821 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     2881 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     2941 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     3001 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     3061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3901 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     3961 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     4021 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     4081 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     4141 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     4201 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     4261 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     4321 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     4381 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4441 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4501 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     4561 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4621 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     4681 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4741 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4801 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4861 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4921 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4981 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     5041 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     5101 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     5161 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     5221 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     5281 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5341 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     5401 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     5461 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5521 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     5581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6121 aatttt
//
