LOCUS V026963 6188 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026963 VERSION V026963 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1055..2350 /label="RNF135(NM_032322)" /note="RNF135(NM_032322)" /gene="RNF135" CDS 2354..2383 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2402..2425 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2445..2461) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2472..3094 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3243..3831) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4160..4207 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4439..5233) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5268..5625) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5627..5731) /gene="bla" /label="AmpR promoter" rep_origin 5758..6186 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgcggc gcgccagatc tcaagcttgc caccatggcg ggcctgggcc tgggctccgc 1081 cgttcccgtg tggctggccg aggacgacct cggctgcatc atctgccagg ggctgctgga 1141 ctggcccgcc acgctgccct gcggccacag cttctgccgc cactgcctgg aggccctgtg 1201 gggcgcccgc gacgcccgcc gctgggcctg ccccacttgc cgccagggcg ccgcgcagca 1261 gccgcacctg cggaagaaca cgctactgca ggacctggcc gacaagtacc gccgcgccgc 1321 acgcgagata caggcgggct ccgaccctgc ccactgcccc tgcccgggct ccagttccct 1381 ctccagcgcg gccgcgaggc cccggcgccg cccggaactg cagcgggtgg cagtagagaa 1441 gagcatcaca gaagttgctc aggagctgac agagctggtg gaacatcttg tagacattgt 1501 cagaagcctg cagaatcaga ggcccctatc agaatctgga ccagacaacg aactgagcat 1561 cctgggcaag gctttttctt ctggggtgga tctttccatg gcttctccaa agctggtgac 1621 ttccgacaca gctgcaggga aaatcagaga tattctccat gacctagaag aaattcagga 1681 aaaattacaa gaaagcgtca cctggaaaga ggctcctgaa gcacaaatgc agggagaact 1741 cctggaagcc ccgtcttcct cctcatgccc attgcctgac cagagccacc ctgcactcag 1801 gagagcttct cggtttgctc agtgggccat ccatccaacc tttaacttga agagcctttc 1861 ctgcagcctg gaggtgtcca aggattcccg tacagtgact gtgtctcacc gcccacaacc 1921 ctatcgctgg agctgtgaga ggttttctac cagccaggtc ttatgttccc aggccctgtc 1981 ttctggaaag cattactggg aagtggacac taggaattgc agccactggg cagttggggt 2041 ggcttcctgg gagatgagcc gcgaccaggt cctgggaagg actatggact cttgttgtgt 2101 ggaatggaag gggactagcc agctctctgc atggcacatg gtcaaggaaa ctgtccttgg 2161 ctcagacaga cctggggtgg tgggcatctg gctgaacctt gaggagggaa agcttgcctt 2221 ctattcagtg gacaatcagg agaagcttct gtatgagtgt accatctctg cctcctctcc 2281 tttgtaccct gccttctggc tgtatggctt acatcctgga aattacctga taataaagca 2341 agtaaaggtg ctcgagcaga aactcatctc agaagaggat ctggcagcaa atgatatcct 2401 ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata gctgtttcct 2461 gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag 2521 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg 2581 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag 2641 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg 2701 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc 2761 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt 2821 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga 2881 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc 2941 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc 3001 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat 3061 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg gcgagcggta 3121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 3181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 3241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 3301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 3361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 3421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 3481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 3541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 3601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 3661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 3721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 3781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 3841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 3901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 3961 aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc cgccccgacg 4021 ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa 4081 acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg 4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt 4201 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc 4261 cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg 4321 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa 4381 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc 4441 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 4501 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 4561 tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag tcgatgaatc 4621 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 4681 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga 4741 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 4801 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 4861 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 4921 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 4981 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 5041 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 5101 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 5161 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 5221 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct 5281 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg 5341 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc 5401 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga 5461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct 5521 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 5581 caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc ttcctttttc 5641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 5701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 6001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 6061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 6121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 6181 cgaatttt //