LOCUS       V031077                 6468 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031077
VERSION     V031077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2615
                     /label="Rab11fip2(NM_001033172)"
                     /note="Rab11fip2(NM_001033172)"
                     /gene="Rab11fip2"
     CDS             2634..2663
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2682..2705
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2725..2741)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2752..3374
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3523..4111)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4440..4487
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4719..5513)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5548..5905)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5907..6011)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6038..6466
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gatgctgtct gagcaagccc aaaagtggtt tccaacccac gtgcaggtca
     1081 cagtgctcca agctaaagat ctgaagccaa aaggcaaaag tggtaccaat gacacataca
     1141 ccataattca gttgggcaaa gagaagtatt ctacatctgt agcagagaaa accctggagc
     1201 cggtctggaa ggaggaggcc tcctttgagc tgcctgggtt gctcatgcag ggtagtccgg
     1261 agaaatacat tctttttctt atagtgatgc acaggtccct ggtgggactg gataagtttt
     1321 tagggcaggt ggcaatcaat ctcaatgaca tctttgagga caaacaaaga aggaaaacag
     1381 agtggtttag attagaatcc aaacaaggaa aaagaataaa aaacaggggt gagataaagg
     1441 tcaatattca atttatgagg aacaacatga cagcaagcat gtttgacttg tcaatgaagg
     1501 acaagacgag atcacctttt gcaaaattaa aagataagat gaaagggagg aaaagcgacg
     1561 gggtgttttc tgatacgtcc tctgccatcg ttccgagcac tcacatgcct gatgccaatc
     1621 ctgagttttc aagtggtgaa atgcagatga aatccaaacc aaaaaagcct tttcttttgg
     1681 gtcctcagcg gctctcttct gcccattcga tgtctgattt aactgggtcc cacttatctt
     1741 ctgagaagct gaagtccagc actgtgggtc caacacatct tctcagtcgc cagatagatt
     1801 cctttggagt tgttccagaa agtggaagtc tcaagtctcc acacagacga acactaagct
     1861 ttgatacttc taaattgaac caacctggca gcattgtgga tgaaggtgaa cactcttttg
     1921 gaagacagag tgacccattt acaaatgtga ctgcttcatt accccaaaaa tttgcaacac
     1981 tgccaaggaa gaagaatcca tttgaagaaa gcagtgagcc atgggacagc agcatgaatt
     2041 tattctcaaa accaattgaa gtcaggaaag aaagtaagag agagaaaagg gagaaggtga
     2101 gcctctttga aagagtgact ggcaagagag atagcaggag acctgacaag ctgaacaatg
     2161 gtggatctga tagcccatgt gacttgaaat cacctagtgc ttttagtgaa aaccggcagg
     2221 actattttga atatgagtca actaacccgt ttacagcaaa attcagggct tcaactataa
     2281 tgccatcttc aagttttcat gtgaatccaa caagcagtga agacctcagg aaaatcccgg
     2341 acaacaatcc tttcgatgcc acggctgggt accggagtct gacctacgaa gaggtgctgc
     2401 aggagctggt gaagcacaaa gaactccttc ggaggaagga cacccatatc cgggagctag
     2461 aggactacat tgacaacctc ctcgtcaggg tgatggaaga gacacccagc atcctgcgag
     2521 tgccctacga gccatccaga aaagctgtat acacccagga ccacttgtca cccgggccac
     2581 cccacctctg tggagaagct acttcaaccc actccacgcg tacgcggccg ctcgagcaga
     2641 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     2701 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     2761 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2821 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2881 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2941 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     3001 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     3061 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     3121 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     3181 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     3241 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     3301 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     3361 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     3421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4261 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     4321 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     4381 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     4441 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     4501 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     4561 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     4621 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     4681 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4741 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4801 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4861 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4921 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4981 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     5041 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     5101 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     5161 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     5221 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     5281 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     5341 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     5401 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     5461 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     5521 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     5581 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     5641 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5701 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5761 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5821 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5881 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     5941 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6001 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     6061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
