LOCUS       V027959                 7659 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027959
VERSION     V027959
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3806
                     /label="Rigi(NM_172689)"
                     /note="Rigi(NM_172689)"
                     /gene="Rigi"
     CDS             3825..3854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3873..3896
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3916..3932)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3943..4565
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4714..5302)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5631..5678
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5910..6704)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6739..7096)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7098..7202)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7229..7657
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gacagcggag cagcggcaga atctgcaagc attcagagac tatatcaaga
     1081 agattctgga ccccacctac atcctcagct acatgagttc ctggctcgag gatgaggagg
     1141 tgcagtacat tcaggctgag aagaacaaca agggcccaat ggaagctgcc tcactcttcc
     1201 tccagtacct gttgaagctg cagtcagagg gctggttcca ggcctttttg gatgccctgt
     1261 accatgcagg ttactgtgga ctttgtgaag ccatcgaaag ttgggacttt caaaaaattg
     1321 aaaagttaga ggaacacaga ttacttttaa gacgtttaga accagaattt aaggccacag
     1381 ttgatccaaa tgatatcctt tctgaactat ccgaatgttt gattaatcag gaatgtgaag
     1441 aaatcagaca gatccgagac actaaaggga gaatggcagg tgcggagaag atggccgaat
     1501 gtcttatcag atccgacaag gaaaactggc caaaggtctt gcaacttgct ttggagaaag
     1561 acaacagcaa gtttagtgaa ttgtggattg ttgataaagg tttcaaaagg gctgaaagca
     1621 aggctgatga ggatgatgga gcggaggcgt ccagcatcca gattttcatt caggaagagc
     1681 cagagtgtca gaatctcagt cagaatcccg ggcctccttc agaagcgtct tctaataatt
     1741 tacacagccc attgaaacca agaaattacc aactggagct tgccctgcct gccaagaaag
     1801 ggaaaaatac aataatatgt gcccctactg gttgtggaaa aacctttgtg tcgcttctta
     1861 tatgtgaaca ccatcttaaa aaattcccat gtggacaaaa agggaaagtg gtcttcttcg
     1921 ctaaccaaat tcctgtctat gagcagcagg caactgtgtt ctcacgatat tttgaaagac
     1981 ttgggtacaa cattgcgagc atttctgggg caacatctga tagcgtctca gtgcagcaca
     2041 tcattgaaga caatgatatc atcatcctga caccccagat tcttgtgaac aatctcaaca
     2101 acggagccat cccctcgttg tctgtcttca ctctgatgat atttgatgag tgtcataaca
     2161 ctagcaaaaa ccacccatac aatcagatca tgttcagata cctagaccac aaacttggag
     2221 agtcacggga cccactgcct caggtcgttg ggctgactgc ctccgtcggc gttggagatg
     2281 ctaagaccgc ggaggaagcc atgcaacata tctgtaaact ctgtgccgcc ctggatgcct
     2341 ccgtgattgc cacagtcaga gacaacgttg cagaactgga acaggtcgtt tataagcccc
     2401 agaaaatttc caggaaagtg gcatcccgga cttcgaacac gtttaaatgc atcatctctc
     2461 agctgatgaa ggagacagag aagctagcca aggatgtctc cgaggaactt ggaaagcttt
     2521 ttcaaattca aaacagagaa ttcggcaccc agaaatatga acagtggatt gtcggcgtcc
     2581 acaaagcgtg ctcagtgttt cagatggcag acaaagagga ggagagccgg gtctgcaaag
     2641 cgctcttcct gtacacatca catttgcgga aatacaacga tgcactcatc atcagtgagg
     2701 atgcacagat gacagacgct ctaaattacc tcaaagcctt cttccacgat gtccgagaag
     2761 cagcattcga tgagaccgag cgagagctta ctcggaggtt tgaagaaaaa ctagaggaat
     2821 tagaaaaagt ttccagggat cccagcaatg agaatcctaa actaagagac ctctacttgg
     2881 tcttacaaga agagtaccac ttaaagccag agaccaagac cattctcttc gtgaagacca
     2941 gagcactcgt ggatgctctg aagaaatgga ttgaagaaaa tcctgcacta agctttctaa
     3001 agcctggcat actgactggg cgtggcagaa caaaccgggc aacaggaatg acgctcccgg
     3061 cacagaagtg tgtgctggag gcattcagag ccagcggaga taacaatatt ctgattgcta
     3121 cctcggtcgc tgatgaaggc attgacattg ctgagtgcaa tctcgtcatt ctctatgagt
     3181 acgtgggcaa cgtcatcaag atgatccaaa ccagaggccg aggaagagca cgagatagca
     3241 agtgcttcct cctgaccagc agcgctgacg tgattgaaaa agaaaaggcg aacatgatca
     3301 aggaaaaaat aatgaatgaa tccatcttaa gactgcagac atgggatgaa atgaaatttg
     3361 gaaagacggt tcaccgcata caggtgaatg aaaaactcct cagagacagt cagcacaaac
     3421 cacaacctgt tcctgacaaa gaaaacaaga aactgctgtg tggaaagtgc aagaattttg
     3481 cgtgctacac agctgacatt cgagtggttg agacgtccca ctacactgtc cttggagacg
     3541 cttttaagga gcgctttgtg tgtaagccac accctaaacc aaagatctat gacaattttg
     3601 agaagaaagc aaagatattc tgcgccaaac agaactgtag ccacgactgg ggaatttttg
     3661 tgagatacaa gacgttcgag attccagtca taaaaattga aagtttcgtc gtggaagata
     3721 ttgtgagcgg agttcagaac cggcactcaa agtggaagga ctttcatttt gaaaggatac
     3781 agttcgatcc tgcagaaatg tccgtaacgc gtacgcggcc gctcgagcag aaactcatct
     3841 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     3901 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     3961 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     4021 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     4081 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     4141 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     4201 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     4261 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4321 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4381 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     4441 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     4501 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     4561 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     5461 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     5521 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     5581 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     5641 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     5701 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     5761 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     5821 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     5881 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     5941 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     6001 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     6061 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     6121 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     6181 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     6241 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     6301 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     6361 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     6421 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     6481 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     6541 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     6601 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     6661 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     6721 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     6781 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     6841 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     6901 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6961 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     7021 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     7081 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     7141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7201 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     7261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
