LOCUS       V040885                 7993 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040885
VERSION     V040885
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2652
                     /label="SLC22A17(NM_020372)"
                     /note="SLC22A17(NM_020372)"
                     /gene="SLC22A17"
     CDS             2659..2685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2689..2715
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2719..2745
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2769..3357
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3423..3471
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3673..4101
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4115..4444
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4511..5305
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5481..5602
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5651..5667)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5675..5691
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5699..5729)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5744..5765
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6053..6641)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6812..7672)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7673..7777)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc tccccgggtg
      721 gccaccggga cccccgagcc caatggcggg ggcggcggca aaatcgacaa cactgtagag
      781 atcaccccca cctccaacgg acaggtcggg accctcggag atgcggtgcc cacggagcag
      841 ctgcagggtg agcgggagcg cgagcgggag ggggagggag acgcgggcgg cgacggactg
      901 ggcagcagcc tgtcgctggc cgtgccccca ggccccctca gctttgaggc gctgctcgcc
      961 caggtggggg cgctgggcgg cggccagcag ctgcagctcg gcctctgctg cctgccggtg
     1021 ctcttcgtgg ctctgggcat ggcctcggac cccatcttca cgctggcgcc cccgctgcat
     1081 tgccactacg gggccttccc ccctaatgcc tctggctggg agcagcctcc caatgccagc
     1141 ggcgtcagcg tcgccagcgc tgccctagca gccagcgccg ccagccgtgt cgccaccagt
     1201 accgacccct cgtgcagcgg cttcgccccg ccggacttca accattgcct caaggattgg
     1261 gactataatg gccttcctgt gctcaccacc aacgccatcg gccagtggga tctggtgtgt
     1321 gacctgggct ggcaggtgat cctggagcag atcctcttca tcttgggctt tgcctccggc
     1381 tacctgttcc tgggttaccc cgcagacaga tttggccgtc gcgggattgt gctgctgacc
     1441 ttggggctgg tgggcccctg tggagtagga ggggctgctg caggctcctc cacaggcgtc
     1501 atggccctcc gattcctctt gggctttctg cttgccggtg ttgacctggg tgtctacctg
     1561 atgcgcctgg agctgtgcga cccaacccag aggcttcggg tggccctggc aggggagttg
     1621 gtgggggtgg gagggcactt cctgttcctg ggcctggccc ttgtctctaa ggattggcga
     1681 ttcctacagc gaatgatcac cgctccctgc atcctcttcc tgttttatgg ctggcctggt
     1741 ttgttcctgg agtccgcacg gtggctgata gtgaagcggc agattgagga ggctcagtct
     1801 gtgctgagga tcctggctga gcgaaaccgg ccccatgggc agatgctggg ggaggaggcc
     1861 caggaggccc tgcaggacct ggagaatacc tgccctctcc ctgcaacatc ctccttttcc
     1921 tttgcttccc tcctcaacta ccgcaacatc tggaaaaatc tgcttatcct gggcttcacc
     1981 aacttcattg cccatgccat tcgccactgc taccagcctg tgggaggagg agggagccca
     2041 tcggacttct acctgtgctc tctgctggcc agcggcaccg cagccctggc ctgtgtcttc
     2101 ctgggggtca ccgtggaccg atttggccgc cggggcatcc ttcttctctc catgaccctt
     2161 accggcattg cttccctggt cctgctgggc ctgtgggatt gtgagcatcc tatcttcccc
     2221 acagtgtggg ctcaacaagg gaaccccaac agagatctga acgaggctgc catcaccact
     2281 ttctctgtcc ttgggctctt ctcctcccaa gctgccgcca tcctcagcac cctccttgct
     2341 gctgaggtca tccccaccac tgtccggggc cgtggcctgg gcctgatcat ggctctaggg
     2401 gcgcttggag gactgagcgg cccggcccag cgcctccaca tgggccatgg agccttcctg
     2461 cagcacgtgg tgctggcggc ctgcgccctc ctctgcattc tcagcattat gctgctgccg
     2521 gagaccaagc gcaagctcct gcccgaggtg ctccgggacg gggagctgtg tcgccggcct
     2581 tccctgctgc ggcagccacc ccctacccgc tgtgaccacg tcccgctgct tgccaccccc
     2641 aaccctgccc tcctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     2701 gtacctgatt atgctagcta tccttatgat gtcccagact acgcatagtg agggcccgat
     2761 atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2821 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2881 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2941 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3001 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3061 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3121 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3181 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3241 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3301 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     3361 gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3421 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     3481 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     3541 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     3601 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta
     3661 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     3841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3961 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4021 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     4081 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4261 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4381 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4441 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4501 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4561 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4621 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4681 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4741 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4801 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4861 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4921 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4981 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5041 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5101 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5161 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5221 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5281 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     5341 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5401 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5461 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5581 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6241 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6481 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6781 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6841 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6901 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6961 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7021 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7081 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7141 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7201 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7261 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7321 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7381 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7441 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7501 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7561 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7621 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7681 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7741 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7801 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg
     7861 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     7921 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     7981 gaatctgctt agg
//
