LOCUS       V046829                 8074 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046829
VERSION     V046829
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2733
                     /label="SLC25A13(NM_001160210)"
                     /note="SLC25A13(NM_001160210)"
                     /gene="SLC25A13"
     CDS             2740..2766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2770..2796
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2800..2826
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2850..3438
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3504..3552
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3754..4182
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4196..4525
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4592..5386
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5562..5683
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5732..5748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5756..5772
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5780..5810)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5825..5846
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6134..6722)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6893..7753)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7754..7858)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggccgccaag
      721 gtggctttaa ccaagagagc agatccagct gagcttagaa caatattttt gaagtatgca
      781 agcattgaga aaaacggtga atttttcatg tcccccaatg actttgtcac tcgatacttg
      841 aacatttttg gagaaagcca gcctaatcca aagactgtgg aacttttaag tggagtggtg
      901 gatcagacca aagatggatt aatatctttt caagaatttg ttgcctttga atctgtcctg
      961 tgtgcccctg atgctttgtt tatggtagcc tttcagctgt ttgacaaagc tggcaaagga
     1021 gaagtaactt ttgaggatgt taagcaagtt tttggacaga ccacaattca tcaacatatt
     1081 ccatttaact gggattcaga atttgtgcaa ctacattttg gaaaagaaag aaaaagacac
     1141 ctgacatatg cggaatttac tcagttttta ttggaaatac aactggagca cgcaaagcaa
     1201 gcctttgtgc aacgggacaa tgctaggact gggagagtca cagccatcga cttccgagac
     1261 atcatggtca ccatccgccc ccatgtcttg actccttttg tagaagaatg tctagtagct
     1321 gctgctggag gtaccacatc ccatcaagtt agtttctcct attttaatgg atttaattcg
     1381 ctccttaaca acatggaact cattagaaag atctatagca ctctggctgg caccaggaaa
     1441 gatgttgaag tgactaagga ggagtttgtt ctggcagctc agaaatttgg tcaggttaca
     1501 cccatggaag ttgacatctt gtttcagtta gcagatttat atgagccaag gggacgtatg
     1561 accttagcag acattgaacg gattgctcct ctggaagagg gaactctgcc ctttaacttg
     1621 gctgaggccc agaggcagca gaaggcctca ggtgattcag ctcgaccagt tcttctacaa
     1681 gttgcagagt cggcctacag gtttggtctg ggttctgttg ctggagctgt tggagccact
     1741 gctgtgtatc ctatcgatct tgtaaaaact cgaatgcaga accaacgatc aactggctct
     1801 tttgtgggag aactcatgta taaaaacagc tttgactgtt ttaagaaagt gctacgctat
     1861 gaaggcttct ttggactgta tagaggtctg ttgccacagt tattgggagt tgccccagag
     1921 aaggccataa aacttacagt gaacgatttt gtgagggata aatttatgca caaagatggt
     1981 tcggtcccac ttgcagcaga aattcttgct ggaggctgcg ctggaggctc ccaggtgatt
     2041 ttcacaaatc ctttagaaat cgtcaagatc cgtttgcaag tggcaggaga aatcaccact
     2101 ggtcctcgag tcagtgctct gtctgtcgtg cgggacctgg ggttttttgg gatctacaag
     2161 ggtgccaaag catgctttct gcgggacatt cctttctcgg ccatctactt tccgtgctat
     2221 gctcatgtga aggcttcctt tgcaaatgaa gatgggcagg ttagcccagg aagcctgctc
     2281 ttagctggtg ccatagctgg tatgcctgca gcatctttag tgacccctgc tgatgttatc
     2341 aagacgagat tacaggtggc tgcccgggct ggccaaacca cttacagcgg agtgatagac
     2401 tgctttagaa agatactgcg tgaagaagga ccaaaagctc tgtggaaggg agctggtgct
     2461 cgtgtatttc gatcctcacc ccagtttggt gtaactttgc tgacttacga attgctacag
     2521 cgatggttct acattgattt tggaggagta aaacccatgg gatcagagcc agttcctaaa
     2581 tccaggatca acctgcctgc cccgaatcct gatcacgttg ggggctacaa actggcagtt
     2641 gctacatttg cagggattga aaacaaattt ggactttacc tacctctctt caagccatca
     2701 gtatctacct caaaggctat tggtggaggc ccactcgagt acccatacga cgtacctgat
     2761 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     2821 tacgcatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     2881 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2941 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3001 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3061 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3121 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3181 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3241 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3301 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3361 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3421 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     3481 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     3541 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     3601 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     3661 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     3721 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3901 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     4141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4201 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4321 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4381 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4441 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4501 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4561 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4621 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4681 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4741 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4801 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4861 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4921 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4981 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5041 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5101 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5161 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5221 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5281 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5341 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5401 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5461 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5521 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5701 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5761 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5821 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5881 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6301 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6901 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6961 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7021 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7081 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7141 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7201 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7261 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7321 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7381 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7441 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7501 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7561 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7621 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7681 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7741 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7801 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7861 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     7921 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     7981 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8041 cttgaccgac aattgcatga agaatctgct tagg
//
