LOCUS       V043501                10803 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043501
VERSION     V043501
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..5483
                     /label="SPATA31D1(NM_001001670)"
                     /note="SPATA31D1(NM_001001670)"
                     /gene="SPATA31D1"
     CDS             5490..5555
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5579..6167
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6233..6281
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6483..6911
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6925..7254
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7321..8115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8291..8412
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8461..8477)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8485..8501
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8509..8539)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8554..8575
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8863..9451)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9622..10482)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10483..10587)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga gaatatcctc tgttttctga
      781 acagctatac tgagacaggg ctgagccctg actcacattg gttggatatc gaccccaact
      841 tcatctgctt gagtgggttg gggttgttta tactgtactt gttctacgtg gtattgaccc
      901 tgtattcgtc acccaccgaa aaaaataatg acatccaaaa gcatcagggc agagccaaga
      961 ggagaaggaa aggtgggaca ttcaaaggtt tcccagactg gaaaagtttc cagagagaag
     1021 aggaagagga aaggaagctg ctttctcttc tgaaaagctt tggacctcct gtttcctgca
     1081 gtcctcgggg ccagcatcat gataccaacc actttcgtcg actgttatgc ccagaccccg
     1141 tctgtcgggt gtgtaagaga gcaactgctg atatccagca actgctgtct tgggagtccc
     1201 tgaaagatgc tgctccctct gtgtcccctt tggcttcttc ggcttctgcg actgagtcat
     1261 cgttcactct ggcttccacc ccctcagcaa cccctccaga agacctaata ctgtcccctc
     1321 ggcctaaggc ctctccacca ccccccttaa ttctctcacc tgacctgatc accaccttag
     1381 ctgacttatt ttcaccctca ccactgaggg accctctgcc accacagcct gtttctccct
     1441 tggattccaa gttccccata gaccattccc caccccaaca gcttcccttt ccccttctcc
     1501 caccacatca cattgagaga gtggagtcca gcctccaacc tgaagccagt ttgtctctga
     1561 acaccatctt ttcatttggc tccaccctat gccaagatat ttcgcaggcc atgaatccca
     1621 ttgattcttg tgctcgtcat cacggaccac caatcccatc tgctttacca ccggaagatt
     1681 gcactgtgac tcagtctaaa tcaagtctca ccatcttgaa gacttttccg gaaatgttat
     1741 ctctaggtgg ctctggtggg tcatccacct ctgccccaac aatcaaaggc attgaccatt
     1801 cacaccttgc atcttcagaa ttcacctggt ggcagcctca tgccaaggac tctttttcct
     1861 ctaattttgt gccatctgat ttcatggagg agcttcttac ccttcattct tctgaggcct
     1921 ttttaggggg gcactctgtg gccaacctca tagagcctgt taacatctca tttctcagcc
     1981 atgacattct ggcactcctg gagagacaag tcaaaaaaag gggtgatttc ctgatgtgga
     2041 aagaaaatgg aaagaaacca ggatcattcc caaaacaact taggccaaac taccaactaa
     2101 attcctcacg gaatatgtta acctcaattg ctgttaagca tgacttggca gaatcctttc
     2161 ctttttgggc cagtaaaggc aaactagaat ggcagcacat ccatcagcag cctccacact
     2221 ctaaatgctt tgaagaccat ttagagcaaa aatatgtcca gctcttctgg ggtctcccat
     2281 ctttgcacag cgagtctctg catcctactg ttcttgtcca acgtggccat tcctccatgt
     2341 ttgtattctt caatggcatt acaaatacat ctatatccca tgaatcccca gtacttcccc
     2401 ctccccaacc tctgtccttg cctagtaccc aaccactacc cttgcctcaa accctgcccc
     2461 aaggtcagtc cccacatctc actcaggtga agtccctggc tcaacctcaa tctccattcc
     2521 gagccctact acctagtcct ctattcctga ttaggatctg tggagtgtgt tttcatagac
     2581 cccagaacga ggcacggtct cttttgccat ctgaaattaa ccatctggag tggaacgtgt
     2641 tgcagaaagt gcaggaaagt ttgtggggct taccctctgt ggttcaaaaa tcccaggaag
     2701 acttttgtcc tccagctccc aatcctgaat tggtcagaaa gtccttcaag gtccatgttc
     2761 cgatctccat cattcctgga gattttccac tcagctctga ggtaaggaag aaactagagc
     2821 aacacattcg aaggaggctc atccagcgca gatggggcct gccccgcaga atccatgagt
     2881 ctctgtcatt gctacgtcct cagagcaaaa tttcagagct atctgtgtca gagagaattc
     2941 atggaccgtt aaatatctct ttggttgagg gtcagaggtg caatgttcta aagaagtccg
     3001 catcaagctt ccctagaagc ttccacgaga ggagctcaaa tatgctttcc atggagaatg
     3061 tggggaatta tcagggatac agccaggaga ctgtcccaaa agatcacctg ttgcatggtc
     3121 cggagacttc ttcagacaag gatctgaggt ctaactctga gagagaccta gaaactcata
     3181 tgatgcatct gtcagggaat gactcagggg tgagactagg tcagaaacaa cttgaaaatg
     3241 ccctgacagt acgtttgagc aagaaatttg aggaaatcaa tgagggtcga atgcctggga
     3301 ctgtgcatag ttcatggcac tcagtcaagc agacaatgtc tcttcctgag aaatcccaca
     3361 gccaaattaa acatcgaaat ctggtaacat tggtgagtga ggaccactgc gttgatactt
     3421 cccaggaaat ttccttcctt agttccaaca aacaaaagat gttggaagcc catattaaaa
     3481 ctttccgtat gaggatgctg tggggccttc ccctcaaggt ccttgaatcc atagaaatct
     3541 tcaaatcgaa agcggacctt tccacttcct tttcccattt cgaccttccc tcctcagcca
     3601 ccttcatctc tcagggagat tccaaagatg gggtctctaa gtcccgtagt cgaagcactt
     3661 ttcaaggaga aaagttggga acaacaagct cagtccccat ccttgatcgt cctcaccctg
     3721 tctcctcacc tgtcgtccaa gaagggcagg ggaccctgag aagacaattt tctgatactg
     3781 accatgacct tatagagaca gattccaaag acggggcctc cacatccctt agaagaggta
     3841 ctacagattt tcaaagcgaa aaattagatt caacaagctc attccccatc ctcggtcatt
     3901 cttaccttgt cacttcacct gtcaaccaag aaaagcaggg gaccctgaga agagaattct
     3961 ctgatactga caatgatctt acagaaagtg tccggacaac agaggatggc agacagactt
     4021 ttctgccccc gccacacagc atcgtagacg aagtcagtca gaaacagact gtactggcca
     4081 gtagatgcag cgcagagctg cccataatgc aagctggagc tggctgtgag tcatgggata
     4141 agagaaagag ttcctttcat aatgtagaca ggcttcaggg cagtagaaag acctttcctg
     4201 tcaccaatgc tcttcaatca caaactagga acaacttgac aaccagcaag tcaggaagct
     4261 gctcactgac aaatgtgaaa gcaagcactt ccaatgaaac tgaaattttc ccaccaagaa
     4321 tatcagttcc tcaagatcct aaatcatcat accttaaaaa tcagatgttg agccagttaa
     4381 agttggtcca gaggaagcat agccaacctc agagccattt cactgacatg tcttttgcct
     4441 tagataactt gagttccaag gacttactga ctaattccca gggcatctcg agtggggaca
     4501 tgggaacttc ccaggtggtg catgtccact tggaggacag cggaatccgt gtggcacaga
     4561 agcaggagcc cagggtccct acctgtgtct tacagaagtg tcaagttacg aatttcccac
     4621 cagctgtaaa cagagtgagt cctgtgagac ccaaaggagg agagcttgat ggaggggatg
     4681 cagggctggg gacatcccaa cgcaggagaa agagcctccc tgttcataac aagacatcag
     4741 gggaggtgct tgggagcaaa tcttccccaa ccttgaaaac acagcctcct cctgaaaacc
     4801 ttttcagaaa atggatgaag acctctttgc agtggtttaa taaacccagc atatcatatg
     4861 aagaacaaga aagttcctgg gaaaagggta gctccctgtc atcatgtgtg cagaatattg
     4921 gtcgagttat aagagctgcc tttactggga ctactgaagc tcagaaaatt aggaaagaca
     4981 ctagggagtt cctagaagag aagctggggc ataggcatgg gatagatatc acctgtcccc
     5041 aagagcccct ttccttccca gtggggcttg ggaaagctca gcacaaccca gaagtgcatg
     5101 tcagagcaga gcctgtccag ggctgtccct gcaactacag ggctccctcc tgcaaagtga
     5161 cacgtaccaa atcttgcagc caacaagcta tctttgttgg ccagaattat cctacaagga
     5221 ttagacagat catagacaag gacagacagc cccagaaagt tgaggcattt aaggggaaga
     5281 tactgtgtca aagccatccc caatccatgc cccacaggaa gcctgtgcca catccaaacc
     5341 ccacttgccg gcgtcaggtc agcctggtgt gtccagccgt cccaaccagt gctaaaagcc
     5401 ctgtgtttag tgatgtgcct ttcctaactg gacagaaaat gcttccaaag catttacagg
     5461 gaggaaaatt tccccccaca aaaggatccg actacaaaga ccatgacggt gattataaag
     5521 atcatgacat cgactacaag gatgacgatg acaagtagtg agggcccgat atctcgacaa
     5581 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5641 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5701 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5761 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5821 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5881 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5941 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6001 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6061 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6121 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg
     6181 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     6241 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6301 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     6361 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     6421 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
     6481 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     6661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6841 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6901 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6961 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7081 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7141 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7201 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7261 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     7321 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7381 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7441 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7501 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7561 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7621 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7681 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7741 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7801 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     7861 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7921 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7981 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8041 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8101 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
     8161 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8221 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8281 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8341 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8401 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8461 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8521 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8581 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8641 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8701 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8761 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     9061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9301 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9601 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9661 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9721 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9781 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9841 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9901 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9961 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10021 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10081 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10141 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10201 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10261 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10321 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10381 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10441 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10621 cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc
    10681 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    10741 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    10801 agg
//
