LOCUS       V038982                 8602 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038982
VERSION     V038982
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3258
                     /label="STAT2(NM_005419)"
                     /note="STAT2(NM_005419)"
                     /gene="STAT2"
     CDS             3265..3354
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3378..3966
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4032..4080
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4282..4710
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4724..5053
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5120..5914
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6090..6211
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6260..6276)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6284..6300
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6308..6338)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6353..6374
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6662..7250)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7421..8281)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8282..8386)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc gcagtgggaa
      721 atgctgcaga atcttgacag cccctttcag gatcagctgc accagcttta ctcgcacagc
      781 ctcctgcctg tggacattcg acagtacttg gctgtctgga ttgaagacca gaactggcag
      841 gaagctgcac ttgggagtga tgattccaag gctaccatgc tattcttcca cttcttggat
      901 cagctgaact atgagtgtgg ccgttgcagc caggacccag agtccttgtt gctgcagcac
      961 aatttgcgga aattctgccg ggacattcag cccttttccc aggatcctac ccagttggct
     1021 gagatgatct ttaacctcct tctggaagaa aaaagaattt tgatccaggc tcagagggcc
     1081 caattggaac aaggagagcc agttctcgaa acacctgtgg agagccagca acatgagatt
     1141 gaatcccgga tcctggattt aagggctatg atggagaagc tggtaaaatc catcagccaa
     1201 ctgaaagacc agcaggatgt cttctgcttc cgatataaga tccaggccaa agggaagaca
     1261 ccctctctgg acccccatca gaccaaagag cagaagattc tgcaggaaac tctcaatgaa
     1321 ctggacaaaa ggagaaagga ggtgctggat gcctccaaag cactgctagg ccgattaact
     1381 accctaatcg agctactgct gccaaagttg gaggagtgga aggcccagca gcaaaaagcc
     1441 tgcatcagag ctcccattga ccacgggttg gaacagctgg agacatggtt cacagctgga
     1501 gcaaagctgt tgtttcacct gaggcagctg ctgaaggagc tgaagggact gagttgcctg
     1561 gttagctatc aggatgaccc tctgaccaaa ggggtggacc tacgcaacgc ccaggtcaca
     1621 gagttgctac agcgtctgct ccacagagcc tttgtggtag aaacccagcc ctgcatgccc
     1681 caaactcccc atcgacccct catcctcaag actggcagca agttcaccgt ccgaacaagg
     1741 ctgctggtga gactccagga aggcaatgag tcactgactg tggaagtctc cattgacagg
     1801 aatcctcctc aattacaagg cttccggaag ttcaacattc tgacttcaaa ccagaaaact
     1861 ttgacccccg agaaggggca gagtcagggt ttgatttggg actttggtta cctgactctg
     1921 gtggagcaac gttcaggtgg ttcaggaaag ggcagcaata aggggccact aggtgtgaca
     1981 gaggaactgc acatcatcag cttcacggtc aaatatacct accagggtct gaagcaggag
     2041 ctgaaaacgg acaccctccc tgtggtgatt atttccaaca tgaaccagct ctcaattgcc
     2101 tgggcttcag ttctctggtt caatttgctc agcccaaacc ttcagaacca gcagttcttc
     2161 tccaaccccc ccaaggcccc ctggagcttg ctgggccctg ctctcagttg gcagttctcc
     2221 tcctatgttg gccgaggcct caactcagac cagctgagca tgctgagaaa caagctgttc
     2281 gggcagaact gtaggactga ggatccatta ttgtcctggg ctgacttcac taagcgagag
     2341 agccctcctg gcaagttacc attctggaca tggctggaca aaattctgga gttggtacat
     2401 gaccacctga aggatctctg gaatgatgga cgcatcatgg gctttgtgag tcggagccag
     2461 gagcgccggc tgctgaagaa gaccatgtct ggcacctttc tactgcgctt cagtgaatcg
     2521 tcagaagggg gcattacctg ctcctgggtg gagcaccagg atgatgacaa ggtgctcatc
     2581 tactctgtgc aaccgtacac gaaggaggtg ctgcagtcac tcccgctgac tgaaatcatc
     2641 cgccattacc agttgctcac tgaggagaat atacctgaaa acccactgcg cttcctctat
     2701 ccccgaatcc cccgggatga agcttttggg tgctactacc aggagaaagt taatctccag
     2761 gaacggagga aatacctgaa acacaggctc attgtggtct ctaatagaca ggtggatgaa
     2821 ctgcaacaac cgctggagct taagccagag ccagagctgg agtcattaga gctggaacta
     2881 gggctggtgc cagagccaga gctcagcctg gacttagagc cactgctgaa ggcagggctg
     2941 gatctggggc cagagctaga gtctgtgctg gagtccactc tggagcctgt gatagagccc
     3001 acactatgca tggtatcaca aacagtgcca gagccagacc aaggacctgt atcacagcca
     3061 gtgccagagc cagatttgcc ctgtgatctg agacatttga acactgagcc aatggaaatc
     3121 ttcagaaact gtgtaaagat tgaagaaatc atgccgaatg gtgacccact gttggctggc
     3181 cagaacaccg tggatgaggt ttacgtctcc cgccccagcc acttctacac tgatggaccc
     3241 ttgatgcctt ctgacttcct cgaggaacaa aaactaatat cagaggaaga tttggaacag
     3301 aaactaatca gtgaagaaga tttagagcaa aagttaattt ccgaggagga cttatagtga
     3361 gggcccgata tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3421 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3481 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3541 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3601 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3661 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3721 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3781 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3841 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3901 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3961 ccccgcctgg aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4021 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     4081 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4141 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4201 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     4261 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     4441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4621 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4741 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4801 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4861 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4921 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4981 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5041 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5101 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5161 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5221 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5281 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5341 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5401 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5461 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5521 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5581 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5641 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5701 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5761 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5821 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5881 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     5941 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6001 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6061 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6241 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6301 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6361 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6421 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6841 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7081 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7381 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7441 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7501 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7561 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7621 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7681 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7741 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7801 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7861 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7921 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7981 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8041 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8101 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8161 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8221 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8281 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8341 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8401 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag
     8461 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     8521 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     8581 ttgcatgaag aatctgctta gg
//
