LOCUS       V021434                 8126 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021434
VERSION     V021434
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             671..2785
                     /label="SYN1(NM_006950)"
                     /note="SYN1(NM_006950)"
                     /gene="SYN1"
     CDS             2792..2818
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2822..2848
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2852..2878
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2902..3490
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3556..3604
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3806..4234
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4248..4577
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4644..5438
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5614..5735
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5784..5800)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5808..5824
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5832..5862)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5877..5898
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6186..6774)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6945..7805)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7806..7910)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgccacc atgaactacc tgcggcgccg cctgtcggac agcaacttta tggccaatct
      721 gccaaatggg tacatgacag acctgcagcg tccgcagccg cccccaccgc cgcccggtgc
      781 ccacagcccc ggagccacgc ccggtcccgg gaccgccact gccgagaggt cctccggggt
      841 cgccccagcg gcctctccgg ccgcccctag ccccgggtcc tcggggggcg gtggcttctt
      901 ctcgtcgctg tccaacgcgg tcaagcagac cacggcggcg gcagctgcca ccttcagcga
      961 gcaggtgggc ggcggctctg ggggcgcagg ccgcggggga gccgcctcca gggtgctgct
     1021 ggtcatcgac gagccgcaca ccgactgggc aaaatacttc aaagggaaaa agatccatgg
     1081 agaaattgac attaaagtag aacaggccga attctctgat ctcaaccttg tggcccatgc
     1141 caatggtgga ttctctgtgg atatggaagt tcttcggaat ggggtgaagg tcgtgcggtc
     1201 tctgaagccg gattttgtgc tgatccgcca gcacgccttc agcatggcac gcaacggaga
     1261 ctaccgcagt ttggtcattg ggctgcagta tgctggaatc cccagtgtta actccttgca
     1321 ttctgtctac aacttctgtg acaagccctg ggtgtttgcc cagatggttc gactgcataa
     1381 gaaactgggg acagaagaat tccctctaat tgatcagacc ttctacccca atcacaaaga
     1441 aatgctcagc agtacaacgt accccgtggt tgtgaagatg gggcacgcac actctgggat
     1501 gggcaaggtc aaggttgaca accagcatga cttccaggac atcgcaagtg tcgtggcact
     1561 gaccaagacg tatgccactg ccgagccctt catcgatgcc aaatatgacg tgcgtgtcca
     1621 gaagattggg cagaactaca aggcctacat gaggacgtca gtgtcaggga actggaagac
     1681 caatactggc tctgcgatgc tggagcaaat tgccatgtct gacagataca agctgtgggt
     1741 ggacacgtgc tcagagattt ttgggggact ggacatctgc gcagtggaag cgctacatgg
     1801 caaggacgga agggatcaca tcattgaggt ggtgggttcc tccatgccgc tcattggtga
     1861 ccaccaggat gaagacaaac agctcatcgt agagctcgtg gtcaacaaga tggctcaggc
     1921 cctgccccgg cagcgacagc gggatgcctc ccctggcagg ggctcccatg gccagactcc
     1981 gtccccaggg gccctgccct tgggccgcca gacctcccag cagcccgcag ggcccccggc
     2041 tcagcagcga cccccaccac agggcggccc tccacagccg ggtccaggcc cccagcgcca
     2101 gggaccccca ttgcagcagc gcccgccccc gcagggccag cagcaccttt caggccttgg
     2161 acccccagct ggcagccccc tgccccagcg ccttccaagt cccacctcag cgccccagca
     2221 gcccgcgtcc caggccgcgc cgccgaccca gggtcaaggc cgccaatccc ggccagtggc
     2281 gggaggcccc ggggcgcctc cagcagcccg cccgcccgcc tctccgtctc cccagcgcca
     2341 ggcgggcccc ccacaggcta cccgtcagac atccgtctct ggcccggctc cgccaaaggc
     2401 ctctggggcc ccaccgggcg ggcagcagcg ccagggcccg ccccagaaac ccccaggccc
     2461 agccggcccc acacgccagg ccagccaggc gggtcccgtg ccccgcactg ggccacccac
     2521 cacgcagcag cctcggccca gcggcccggg ccccgctgga cgtcccaaac cacagctggc
     2581 ccagaaaccc agccaggacg tgccgccacc cgccaccgcc gctgcagggg gacctccgca
     2641 cccccagctc aacaaatccc agtctctgac caatgccttc aaccttccag agccagcccc
     2701 gcccaggccc agccttagcc aggacgaggt gaaagctgag accatccgca gcctgaggaa
     2761 gtctttcgcc agcctcttct ccgacctcga gtacccatac gacgtacctg attatgcaac
     2821 ttatccttat gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata
     2881 gtgagggccc gatatctcga caatcaacct ctggattaca aaatttgtga aagattgact
     2941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3301 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3481 gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     3541 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     3601 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     3661 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     3721 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc
     3781 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3961 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4141 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     4201 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4261 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4321 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4501 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4561 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4621 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4681 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4741 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4801 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4861 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4921 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4981 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5041 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5101 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5161 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5221 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5281 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5341 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5401 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa
     5461 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5521 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5581 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5701 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5761 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5941 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6361 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6601 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7921 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc
     7981 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     8041 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     8101 acaattgcat gaagaatctg cttagg
//
