LOCUS       V019399                 6477 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019399
VERSION     V019399
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..2707
                     /label="Senp3(NM_030702)"
                     /note="Senp3(NM_030702)"
                     /gene="Senp3"
     CDS             2708..2725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     primer_bind     complement(2734..2750)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2761..3383
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3532..4120)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4449..4496
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4728..5522)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5557..5914)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5916..6020)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6047..6475
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgaaag agactataca
     1021 ggggaccggg tcttgggggc ctgagcctcc gggacccggc accacttact caaatcccag
     1081 gcgagagcgt cttcgttggc ccctaccccc taagccccgg ctcaagtccg gtggtggttt
     1141 tgggccagat cctgggtctg ggaccacagt gccaactaga cgcctccctg ccccccggcc
     1201 atcttttgat gcctcagcta gtgaagaaga ggaagaggaa gaggaggaag atgaggagga
     1261 agtagcagct tggaggctac cccctaggtg gggccaactg ggggcctccc agcgctctcg
     1321 agctctccga ccctctcata gaaaaacctg ctcacagcgc cggcgccgag ccatgagagc
     1381 cttccagatg ctgctctact caaaaagcac ctcgctgaca ttccactgga agctttgggg
     1441 gcgccaccga ggccggcggc ggaacctcgc gcaccccaag aaccatctct caccccagga
     1501 agggggtgca acgccacagg tgccatcacc ctgctgtcgt tttgactccc cccgggggct
     1561 acccccaccc cggctgggtc tgctaggtgc tctcatggca gaggatggga tgagagggtc
     1621 tccaccagtg ccctctgggc cccccatgga ggaagatgga ctaaggtgga ccccaaagtc
     1681 tcctctggac cctgactcgg gcctcctctc atgtactcta cccaatggtt ttgggggact
     1741 gtctggtcct gaaggggagc gcagcctggc accccctgat gccagcatac tcatcagtaa
     1801 tgtgtgcagc attggagacc acgtggctca ggaacttttt cagagctctg acttgggcat
     1861 tgcagaagag gcagatcgga ctggggagaa agctggccag catagccccc ttcgggagga
     1921 acatgtgacc tgtgtgcaga gtatcttaga tgaattcctt caaacttatg gcagcctcat
     1981 ccctctcagc actgatgaag ttgtagagaa gttggaggac attttccagc aggagttctc
     2041 tacaccctca aggaagagtc tggtactaca gctgatccag tcttatcagc ggatgccagg
     2101 caacgctatg gtaaggggct tccgggtatc ctataagcga cacgtgctca ccatggatga
     2161 cttgggtacc ttatatggac agaactggct caatgaccag gtgatgaaca tgtatggaga
     2221 tctggtcatg gacacagtcc ctgaaaaggt gcatttcttc aacagcttct tctatgataa
     2281 actccgtacc aagggttatg atggggtaaa gaggtggacc aaaaatgtgg acatcttcaa
     2341 taaggaatta ctgctaatcc ccatccatct ggaggtgcac tggtccctta tctcagttga
     2401 tgtaaggcga cgtaccatca cctattttga ctcccagcga actctaaatc gccgctgccc
     2461 taagcatatt gccaagtatc tacaggcaga ggcagtcaaa aaagaccgac tggacttcca
     2521 tcagggctgg aaaggttact tcaaaatgaa tgtggccagg cagaataatg acagtgactg
     2581 tggtgccttt gtattacagt actgcaagca cctggccctg tctcagccat tcagcttcac
     2641 ccagcaggac atgcccaaac tccgtcgtca gatctacaag gaactgtgtc actgcaaact
     2701 cactgtgcat catcaccatc accattgacc gcggtcatag ctgtttcctg aacagatccc
     2761 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2821 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2881 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2941 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3001 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3061 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3121 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3181 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3241 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     3301 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     3361 actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc
     3421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3721 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3961 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4261 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     4321 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     4381 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     4441 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     4501 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     4561 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     4621 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4681 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     4741 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     4801 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     4861 atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     4921 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     4981 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     5041 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5101 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5161 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5221 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5281 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     5341 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5401 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5461 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     5521 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     5581 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     5641 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5701 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5761 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     5821 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     5881 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca atattattga
     5941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     6061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaatttt
//
