LOCUS       V022750                10100 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022750
VERSION     V022750
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..1399
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EYFP"
     CDS             1406..4822
                     /label="Senp6(NM_001311110)"
                     /note="Senp6(NM_001311110)"
                     /gene="Senp6"
     CDS             4829..4855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4876..5464
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5530..5578
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5780..6208
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6222..6551
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6618..7412
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7588..7709
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7758..7774)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7782..7798
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7806..7836)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7851..7872
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8160..8748)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8919..9779)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9780..9884)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      721 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      781 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      841 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc
      901 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      961 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1021 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1081 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1141 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1201 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1261 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1321 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1381 catggacgag ctgtacaagg aattcatggc ggcgggcaag agcggtggca gcgctggggc
     1441 tcttttcctg aaagctttgg atagatcaga atctaagagg gatggaggct ttaaaaataa
     1501 ttggagcttt gatcatgaag aagaaagtga aggagatgca gacaaagatg gggcaaatct
     1561 actcagtgta gaagatgagg attctgaaat ctcaaaagga aaaaagttaa atcggagatc
     1621 tgaaattgtt gctactagtt ctggtgattt catcttgaag acttatgtaa ggcggagcaa
     1681 gactgacggc tttaaaactt tgaaaggcaa cccaattgga cttaacatgt taagcaacaa
     1741 taagaaactg agtgaaagca ccgcaggcac agcattatgt tctggaactg tggttcatgg
     1801 gaggcgcttc catcatgctc actcacagac gccaggcatc agaacagcgg ctcagagcaa
     1861 tcaggaccga aaagaaagga aagaataccc accttatgtc cacaaagctg aaaacagccc
     1921 tgtaatgcta agtcatggcc aaggtggaga ccatataatg aagaaaactg aagagtcaga
     1981 atcatatgta gagtctgaga ttaagaggaa agtccagcag aagcggcact gtagcactta
     2041 ccagctttct cctctgtccc ctgcctcaaa gaagtgtttg acccatttag aggtttctga
     2101 acagcgtgaa tattgcccaa aatgtggaaa agaaaaagaa aatcagacca aatgccaaag
     2161 ttgtggtatt gtttttcata atgatttgca aagaaattgc cgacaagccg taactttgaa
     2221 tgaacctact ggaccattgc taagaacatc aattcatcag aactctggag gacagaaatc
     2281 acaaaacaca ggactaacag ctaagaaatt ttatgggaac agtgtggata agattccaat
     2341 tgatattttg gtcacctgtg atgatagcag acataattac atacagacaa atgggaaagt
     2401 cattttacct ggaggaaaaa tacctaaact cacaaaccca aaagagcgaa agataagcgt
     2461 gtcagattta aacgatccaa tcattttgtc tagtgatgat gatgacgacg acgacgatag
     2521 gactaagaga agagagagca cgtccccgaa gcctgccgac tcagcatgct cctcaccagt
     2581 accatctacg ggaaaagttg aagctgcact aaatgcagat gcctgtagag cagagcagga
     2641 acccagaagc agtccagcag agcctgagtt aaataccatt gtcataccac gaaaagcaag
     2701 aatgaaggac cagcttggta attccatcag cacaccttta aaacgtcgta aagtaaacag
     2761 tcatgcagct tttattcacc ctatgtcttt aagttgccaa aactttgaaa gtgtcatttt
     2821 aaactgccga agtatacgag ttggaacact cttccgactg ttggtagagc ctgtaatttt
     2881 ttctttagag tcaatcacga tacatctaga tggaccagaa agtgatcctg tagacattat
     2941 tttaaatacc tctgatctaa ctaaatgtga atggtgtaat gtccggaaat taccagtagt
     3001 gtttctccag gcaataccag cagtttacca gaagctgagc atgcagctac agatgagtaa
     3061 agaagataaa gtttggaatg attgtaaagg aataaacagg ataacaagtt tagaagaaca
     3121 gtacattatt ttaatttttc aaactggcct tgatcatcag gcagaggtgg tctttgaaag
     3181 tatcattact gacattggta taaggaataa tgttcccaac ttttttgcaa aaattctttt
     3241 tgacgaagcc aatagcagac ttgttgcctg tacaagaagc tatgaagaga gcatcaaagg
     3301 aaactgcgcg caaaaagaga acaaagtgaa aactgtgtcg tttgaatcta aaatacaact
     3361 tagaagcaaa caagaattgc agttttttga tgatgacgaa gaagctgggg agagccatac
     3421 aatcttcatt ggccctgtag aaaaattgat agtataccca ccacctccag ctaagggagg
     3481 catttctgtt actaatgagg acctgcactg tctaagtgaa ggagagtttt taaatgatgt
     3541 tattatcgac ttttatttga aatacttggt gcttgaaaaa ctgaagaaag aagacgctga
     3601 ccgaattcat atatttagtt cttttttcta taaacgcctt aatcagagag agaggagaaa
     3661 tcctgagaca actaatctgt cgatacaaca aaaacggcat gggagagtaa aaacgtggac
     3721 acggcatgta gatatttttg agaaggactt tatttttgta ccccttaatg aagctgcaca
     3781 ttggtttttg gctgttgttt gtttccctgg tttggaaaaa ccaaaatatg aacctaatcc
     3841 tcattaccat gaaaatgcag tcatgcaaaa aactccaagt gcagaggaca gttgtgtttc
     3901 ttctgccagt gagatgggtg cttgttcaca gaactctgct gccaagcctg tgatcaagaa
     3961 gatgttaaat agaaaacatt gcttagctgt gactgattcg agtgctgcac aggaagaaag
     4021 tgagccttgt taccggagaa acgcatacag tgtaaagtgt agtatgaaaa aaaaaaatca
     4081 tgctatcaat gaaaatgaag aaccaagtaa cggagaatct acgtgccagg acatttgtga
     4141 tagaacccaa agtgagaatg gcctccggga tgaatgcttc agttctgtac atcatccaga
     4201 tgccttaagc aaaatcagac taaactacgg tgatcagtca gcagacggtg ggaagctgct
     4261 tgaagatgag ctcattgact tctcagagga tcaggatgac ccggatgata gcagtgatga
     4321 cggactcctt gctgatgaaa actacagttc agaaatagga cagtggcatt taaagcctac
     4381 tgtctgtaaa caaccttgta tcctccttat ggactcactc agaggccctt ctcgttcaaa
     4441 tgttgtcaaa attctaagag agtatttaga agtggaatgg gaggtgaaaa aaggaagcaa
     4501 aagaagtttt tccaaagatg ttatgaaagg gtccaaccca aaagtcccac aacagaacaa
     4561 cttcagtgac tgtggtgtct atgtgctgca gtatgtagag agcttttttg agaatccagt
     4621 actaaatttt gagctcccta tgaatttgat gaactggttt cctcccccca gaatgaaaac
     4681 aaaaagagaa gaaatccgaa acataatttt gaagctacag gaatcacaga gtaaagacaa
     4741 gaagctgctg aaggactcct tagcagagac gtccttaggg gacggagcag agcagtacgc
     4801 cagcgccagc ggtggctcag agctcgagta cccatacgat gttccagatt acgcttaggg
     4861 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4921 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4981 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5041 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5101 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5161 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5221 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5281 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5341 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5401 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5461 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     5521 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     5581 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5641 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5701 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg
     5761 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5821 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     5941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6121 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     6181 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6481 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6541 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6601 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6661 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6721 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6781 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6841 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6901 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6961 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7021 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7081 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7141 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7201 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7261 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7321 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7381 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc
     7441 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7501 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7561 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7621 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7681 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7741 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8341 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8581 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9901 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta
     9961 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    10021 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    10081 gcatgaagaa tctgcttagg
//
