LOCUS       V031108                 6168 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031108
VERSION     V031108
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2315
                     /label="Septin14(NM_028826)"
                     /note="Septin14(NM_028826)"
                     /gene="Septin14"
     CDS             2334..2363
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2382..2405
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2425..2441)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2452..3074
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3223..3811)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4140..4187
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4419..5213)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5248..5605)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5607..5711)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5738..6166
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcggaaaaa ccaactaaca cgtcggtgcc aatacctggc agtgaagatc
     1081 cacaaaagga gaacatccgt tgcttatcca ctcttgggca ttttgggttt gaatgtttgc
     1141 ccactcagct ggtgaacaaa tcaatccaga aaggattctc ttttaatatt ctctgtgtgg
     1201 gagaaactgg aattggaaaa acaaccctga tcaatacact gttcaataca aatctaaaag
     1261 agaccaagtc ctctcatttc tactcgaagg ttggactgac ggtgaagaca tatgaacttc
     1321 tggaaagaaa tattccactg agattgactg tcgtgaagac agttgggtat ggtgatcaga
     1381 taaacaagga agccagctac cagccagtcg ttgactacct agatgctcag tttgaagcct
     1441 acctccagga ggagctgaag atcaagcgct ccttagctga ctatcacgat tctcgcatcc
     1501 atgtctgcct ttatttcatc acccctacag gacattccct caagtccctg gacctgctaa
     1561 caatgaagag cattgacaga agggtgaaca tcataccctt gattgccaaa gctgactcgc
     1621 tttcaaagaa cgacctacag aggttcaaga acaacataat gagtgagctg aacagcaatg
     1681 gcatccagat atatcagttc caggtcgatg acgaagcgag tgctcaagtg aacagctcgg
     1741 ggctgttacc atttgctgta gtaggaagta tggaggaggt caaagttgga aagagaatgg
     1801 tcagaggccg ccattaccct tggggagttt tgcaagtgga aaatgaaaac cactgtgact
     1861 ttgtgaagct ccgggatttg cttctgtcca ctaatatgga agatctgaaa gatcaaaccc
     1921 acactcagca ttatgaatgt tacaggagca acaggctgca gaagctgggc ttcagtgaca
     1981 cgggcccaga caaccggcca gtgagttttc aagagatgta tgaagccaag agacgagagt
     2041 tccacaatca atgtcagaag gaggaggaag aactcaagca gacgttcatg cagcgagtca
     2101 aggagaagga gttgaccttt aaggatgcgg agaaggagct gcaggacaag ttcgagcacc
     2161 tgaagagaat ccagcaagag gagatcctga aactggagga agagaggaga aaattagaag
     2221 aacaaatcat agatttttat aagatgaagg ctgcttctga gtcagcacag gctcaggtgt
     2281 gcaccaacat aaagaaggac aaagatcgta agaagacgcg tacgcggccg ctcgagcaga
     2341 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     2401 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     2461 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2521 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2581 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2641 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2701 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2761 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2821 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2881 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     2941 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     3001 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     3061 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3661 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3961 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     4021 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     4081 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     4141 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     4201 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     4261 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     4321 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     4381 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4441 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4501 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4561 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4621 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4681 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4741 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4801 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     4861 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     4921 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4981 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     5041 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     5101 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     5161 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     5221 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     5281 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     5341 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5401 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5461 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5521 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5581 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     5641 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5701 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
