LOCUS V030055 6387 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030055 VERSION V030055 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2534 /label="Sgsh(NM_018822)" /note="Sgsh(NM_018822)" /gene="Sgsh" CDS 2553..2582 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2601..2624 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2644..2660) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2671..3293 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3442..4030) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4359..4406 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4638..5432) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5467..5824) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5826..5930) /gene="bla" /label="AmpR promoter" rep_origin 5957..6385 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gcactgcccg ggactggcct gctgcacaat tctgttggtc ctgggactct 1081 gcggcgcgca ctcgcgcaac gtgctactga tagttgcgga tgacggaggc tttgagagtg 1141 gtgtatacaa caacactgcc atcgccaccc ctcacctgga tgccctgtcc cgccacagcc 1201 ttatcttccg taacgccttc acgtctgtca gcagctgttc cccgagccgt gccagcctcc 1261 tcaccggcct gccccagcat cagaatggca tgtatgggct gcaccaggat gtgcatcact 1321 tcaactcttt tgacaaggta cagagccttc cgctgctgct caaccaggcc ggagtgcgca 1381 caggcatcat tgggaagaag cacgtgggtc cggagacggt gtatcccttt gactttgcat 1441 tcacagagga gaacagctct gtgatgcagg tggggcggaa catcactaga attaagcaac 1501 tggtccagaa atttctgcag actcaggatg acaggccctt cttcctgtac gtggctttcc 1561 atgacccgca ccgctgtggg cactctcagc cccagtatgg aaccttctgt gagaagtttg 1621 gcaacggaga gagtggaatg gggtacattc cagactggac accccagatc tacgaccctc 1681 aggatgtgat ggtgccctac tttgtcccgg acacaccagc agcccgagca gacctagctg 1741 ctcagtacac caccatcggg cggatggacc aaggggtagg tctggtgctc caggagctgc 1801 gaggtgctgg tgtgctgaac gacaccctca tcatcttcac atctgacaat ggtatccctt 1861 tccccagcgg caggaccaac ctgtactggc ccggtacagc cgagcctttg ctggtgtcat 1921 ctccagagca cccacagcgc tggggccagg tcagcgacgc ctacgtgagc cttctagacc 1981 tcacccctac catcctggac tggttctcca tcccgtaccc cagctatgcc atctttggct 2041 caaagacgat ccagctcaca ggccgatccc tcctgccggc gctggaggca gagccccttt 2101 gggccactgt cttcagcagc cagagccacc acgaggtcac catgtcctac ccgatgcgct 2161 cggtgtacca ccagaacttc cgcctcattc acaacctgag cttcaagatg ccatttccca 2221 tcgaccaaga tttctatgtc tcgccgacct tccaggacct cctgaaccga accaccacag 2281 gccggcccac gggctggtac aaggacctcc accgttacta ctaccgggaa cgctgggaac 2341 tctacgacat cagccgggac cctcgagaga cacggaacct ggccgctgac ccagacttgg 2401 ctcaagtgct ggagatgctg aaagctcagc ttgtcaagtg gcaatgggag acacatgacc 2461 cctgggtgtg cgccccagat ggagtcctgg aggaaaagct cacaccccag tgccgaccgc 2521 ttcacaatga actcacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 2581 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 2641 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 2701 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2761 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2821 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2881 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2941 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 3001 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 3061 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 3121 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 3181 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 3241 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 3301 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 4201 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 4261 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 4321 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 4381 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 4441 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 4501 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 4561 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 4621 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 4681 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 4741 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 4801 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 4861 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 4921 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 4981 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 5041 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 5101 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 5161 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 5221 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 5281 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 5341 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 5401 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 5461 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 5521 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 5581 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 5641 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 5701 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 5761 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 5821 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5941 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 6001 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 6061 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 6121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 6181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 6241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 6301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 6361 atttaacaaa aatttaacgc gaatttt //