LOCUS       V030015                 6525 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030015
VERSION     V030015
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2672
                     /label="Slc11a1(NM_013612)"
                     /note="Slc11a1(NM_013612)"
                     /gene="Slc11a1"
     CDS             2691..2720
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2739..2762
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2782..2798)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2809..3431
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3580..4168)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4497..4544
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4776..5570)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5605..5962)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5964..6068)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6095..6523
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gattagtgac aagagccccc cgaggctgag caggcccagt tatggctcca
     1081 tttccagcct gcctggccca gcacctcagc cagcgccttg ccgggagacc tacctgagtg
     1141 agaagatccc cattcccagc gcagaccagg gtacattcag cctgaggaag ctgtgggcgt
     1201 tcacggggcc tggtttcctc atgagcatcg ctttccttga cccgggaaac attgagtccg
     1261 accttcaagc tggcgctgtg gctgggttca aactcctctg ggtgctgctc tgggcgactg
     1321 tgctaggttt gctgtgccag cggctggctg cccggctggg cgtggtgaca ggcaaggact
     1381 tgggtgaagt ctgccatctc tactacccca aggtgccccg catcctcctc tggctgacca
     1441 ttgagctggc cattgtgggc tcagatatgc aggaagtcat cgggacggct atctccttca
     1501 atctgctctc cgctggacgc atcccgctgt gggacggtgt actgatcacc attgtggaca
     1561 ccttcttctt cctcttcttg gataactatg gtttgcgcaa gctggaagct ttcttcggtc
     1621 tcctcattac cataatggct ttgaccttcg gctatgagta tgtggtagca cacccttccc
     1681 agggagcgct ccttaagggc ctggtgctgc ccacctgtcc gggctgtggg cagcccgagc
     1741 tgctgcaggc agtgggcatc gtcggtgcca tcatcatgcc ccataacatc tacctgcact
     1801 cagccttggt caagtctaga gaagtagaca gaacccgccg ggtggatgtt cgagaagcca
     1861 acatgtactt cctgattgag gccaccatcg ccctatcggt gtccttcatc atcaacctct
     1921 tcgtcatggc tgtttttggt caggccttct accagcaaac caatgaggaa gcgttcaaca
     1981 tctgtgccaa cagcagcctc cagaactatg ctaagatctt ccccagggac aataacactg
     2041 tgtcagtgga tatttatcaa ggaggtgtga tcctaggctg tctctttggc cctgcggccc
     2101 tctacatctg ggcagtaggt ctcctggcag cggggcagag ttctactatg accggcacct
     2161 atgcaggaca gttcgtgatg gagggtttcc ttaagctgcg gtggtcccgc ttcgctcggg
     2221 tccttctcac gcgctcttgc gccatcctgc ccactgtgtt ggtggctgtc ttccgagacc
     2281 tgaaggacct gtccggcctc aacgatctac tcaatgttct gcagagtcta ctgctgccct
     2341 tcgctgtact gcccattttg actttcacca gcatgccagc tgtcatgcag gagtttgcca
     2401 acggccggat gagcaaagcc atcacttcgt gcatcatggc gctagtctgc gccatcaacc
     2461 tgtactttgt gatcagctac ctgcccagcc tcccgcaccc tgcctacttt ggccttgtgg
     2521 ctctgttcgc aataggttac ttgggcctga ctgcttatct ggcctggacc tgttgcatcg
     2581 cccacggagc caccttcctg acccacagct cccacaagca cttcttatat gggctcccta
     2641 acgaggagca gggaggcgtg cagggttccg ggacgcgtac gcggccgctc gagcagaaac
     2701 tcatctcaga agaggatctg gcagcaaatg atatcctgga ttacaaggat gacgacgata
     2761 aggtttaaac ggccggccgc ggtcatagct gtttcctgaa cagatcccgg gtggcatccc
     2821 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     2881 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     2941 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3001 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3061 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3121 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3181 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3241 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     3301 ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg
     3361 ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg
     3421 cctgttgaat tgggtacgcg gccagcggcg agcggtatca gctcactcaa aggcggtaat
     3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4321 gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     4381 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     4441 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     4501 aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
     4561 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc
     4621 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     4681 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     4741 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4801 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4861 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4921 cgatccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4981 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     5041 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     5101 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     5161 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     5221 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     5281 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     5341 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc
     5401 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     5461 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     5521 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     5581 cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca
     5641 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     5701 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     5761 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5821 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     5881 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     5941 cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag
     6001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6061 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     6121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6361 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6421 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6481 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga atttt
//
