LOCUS       V030340                 6891 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030340
VERSION     V030340
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3038
                     /label="Slco1a1(NM_013797)"
                     /note="Slco1a1(NM_013797)"
                     /gene="Slco1a1"
     CDS             3057..3086
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3105..3128
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3148..3164)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3175..3797
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3946..4534)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4863..4910
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5142..5936)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5971..6328)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6330..6434)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6461..6889
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggaagaaaca gagaaaaagg ttgcaacaca agaaggcaga ttcttctcca
     1081 agatgaaggt gtttctgatg tcattaacat gtgcatacct agccaaatca ctatcaggag
     1141 tttatatgaa ttccatgctc acacaaatag agagacaatt tggtatccct acatctgtag
     1201 ttggatttat cactgggagc tttgagatag gaaacctttt gttgattgta ttcgtgagtt
     1261 attttggaag gaaactgcac agacctatca ttattggtgt tggatgtgtg gttatgggcc
     1321 tggggtgttt cttaatggca tcacctcatt tcctcatggg cagatacaaa tatgaaacaa
     1381 caatatcacc tacaagcaac ttgtcctcaa acagcttctt gtgtatagaa aacagaacac
     1441 agaccttaaa gccaacgcaa gatccaacag agtgtgtaaa agaaataaaa tcattaatgt
     1501 ggatatatgt actaatagga aatactatgc gtggaattgg tgaaactccc ataatgccct
     1561 tgggtatttc ttacatcgaa gactttgcca aatcagaaaa ctctccttta tatattggga
     1621 ttttagaaat ggggaagatt gttggcccga ttattggact tttgttggga tctttctttg
     1681 cacgtgttta tgtagacatt gggtctgtga atacagatga cctgaccata actcccactg
     1741 atacacgctg ggtcggtgct tggtggattg gctttttggt ctgtgcagga gtgaatatcc
     1801 tgaccagcat cccctttttc ttctttccaa agacactgcc aaagaaagaa ctacaggata
     1861 atgtggatgt gactaaatat gagaaagttg aaaaacatag agaaagagcc aaaaaggaaa
     1921 accttggaat cactaaagat ttcttgccat tcatgaagag cctctgctgc aatcctattt
     1981 atatgctgtt cagtcttacg agtgtgctcc agataaatgg atttgccagt acatttacct
     2041 tcttgcccaa atacctggag cagcaatatg gaaaatccac ttcagaggca gtattcctca
     2101 taggtgttta tagcttgcct ccagtatgcc ttggatattt aatcagtggc tttattatga
     2161 agaagtttaa gatcactgtc aagaaagctg catacatagc atttggcctg tccttatctg
     2221 aatattttat ttttctctgt aactatttgt tgacctgtga taatttccca gttgctggat
     2281 taaccacctc ttataaagga gttcagcacc ctctatatgg ggagaaaaat gtccttgctg
     2341 actgcaacac aaggtgcagc tgcttaacgg acacatggga tccagtgtgt ggggacaatg
     2401 gcctagctta catgtcagcc tgcctcgcag gctgtgagaa gtctgttgga actggaacca
     2461 acatggtgtt tcaaaattgc agctgcattg ggtcatcagg aaactcatct gcagtcctgg
     2521 ggctgtgtaa gaaaggccct gagtgtgaca acaagctgca gtacttttta atcaagtcag
     2581 tattttccag tttcatcttc tcactcgcag ccatccctgg atacatggtt cttctgaggt
     2641 gtgtcaaatc tgaagagaag tcaattggag ttggattaca tgcatttttc ataagactat
     2701 tagctggcat tccggcacct gtttactttg gcgctttgat agacagaacc tgtttacatt
     2761 ggggaactct gaaatgtggt caaccagggg catgcaggat gtatgatata aacagattca
     2821 ggcacattta cctggggttg cctgcagcag tgagaggatc aagctttctc cccgcagtct
     2881 tcattctaat ccttatgagg aaattccatt tccctggcga catccactct ccagacactg
     2941 aacttgcaga gatgaagctc acagagaagg aaagcgagtg cacagatgtg tgcagaagtc
     3001 ctaaggtcga gaacgatgga gaactgaaaa ctaagctgac gcgtacgcgg ccgctcgagc
     3061 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     3121 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     3181 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3241 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     3301 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     3361 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     3421 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     3481 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     3541 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     3601 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     3661 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     3721 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     3781 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     3841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4381 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4681 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     4741 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     4801 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     4861 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     4921 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     4981 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     5041 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     5101 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     5161 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     5221 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     5281 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     5341 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     5401 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     5461 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     5521 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     5581 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     5641 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     5701 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     5761 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     5821 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5881 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     5941 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     6001 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     6061 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     6121 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     6181 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     6241 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     6301 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     6361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6421 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
