LOCUS V029858 5211 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029858 VERSION V029858 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1358 /label="Slurp1(NM_020519)" /note="Slurp1(NM_020519)" /gene="Slurp1" CDS 1377..1406 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1425..1448 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1468..1484) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1495..2117 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2266..2854) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3183..3230 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3462..4256) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4291..4648) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4650..4754) /gene="bla" /label="AmpR promoter" rep_origin 4781..5209 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gacccttcgc tgggccatgt ggctgctgct cttggcagcc tggagcatgg 1081 gctatggtga ggccttccga tgctatacct gtgagcagcc cacggccatt aactcatgca 1141 agaatattgc tcagtgcaag atggaagaca cagcctgtaa gactgtactg gagacagtgg 1201 aagcagcgtt ccccttcaac cacagtccca tggtgacccg ctcctgctcc agctcgtgtc 1261 tggccaccga ccctgatggc attggcgttg cccatcctgt cttctgttgc ttccgtgacc 1321 tctgcaactc agggtttcca ggcttcgtgg caggcctcac gcgtacgcgg ccgctcgagc 1381 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg 1441 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg 1501 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc 1561 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat 1621 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt 1681 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact 1741 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga 1801 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc 1861 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct 1921 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta 1981 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa 2041 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag 2101 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc 2161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 2221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 2281 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 2341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 2401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 2461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 2521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 2581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 2641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 2701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 2761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 2821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 2881 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 2941 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3001 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg 3061 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc 3121 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt 3181 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg 3241 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa 3301 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 3361 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 3421 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 3481 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 3541 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 3601 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 3661 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 3721 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 3781 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 3841 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 3901 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 3961 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 4021 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 4081 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 4141 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 4201 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 4261 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc 4321 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 4381 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 4441 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 4501 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 4561 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 4621 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt 4681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4741 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 4801 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 4861 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 4921 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 4981 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 5041 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 5101 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 5161 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t //