LOCUS V029884 5661 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029884 VERSION V029884 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1808 /label="Sun3(NM_001290520)" /note="Sun3(NM_001290520)" /gene="Sun3" CDS 1827..1856 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1875..1898 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1918..1934) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1945..2567 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2716..3304) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3633..3680 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3912..4706) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4741..5098) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5100..5204) /gene="bla" /label="AmpR promoter" rep_origin 5231..5659 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gcctaaggag caacaggaac ttttaaagaa agaaagccag actttggaga 1081 acaattttcg tgaaattctg tttttaattg aacaaataga tgttctgaag gcgttgctta 1141 aagacatgaa ggacggcgta cacaatcaca gcttgcctgt tcaccgagat gctgtgcagg 1201 accaggccac tactgacgtt ctagatgagg aaatgtcaaa cttggtacat tatgtgctta 1261 aaaagttcag aggggaccaa atacagttgg ctgattatgc cttgaagtca gcaggagcct 1321 ctgtcattga agctgggacc tcagaaagtt acaaaaataa taaagcaaaa ctgtactggc 1381 atggcatagg gttcctcaac tatgagatgc ctccggatat gatacttcag ccagatgtcc 1441 accctgggaa gtgctgggcc tttccaggtt cccagggtca catcctgatt aagctcgcca 1501 ggaagatcat cccaacagca gttaccatgg agcacatctc ggagaaggtg tccccttcag 1561 gaaacatctc aagtgcaccc aaggagttct ctgtctatgg cgttatgaag aaatgtgaag 1621 gagaagaaat attcctaggt cagtttattt ataacaaaat ggaagcaacc attcaaacat 1681 ttgagctcca gaatgaagct tctgaatctt tgctatgtgt aaaacttcaa atccttagca 1741 actggggaca tccaaagtat acgtgtttgt accgatttag agtccacggc atccccagtg 1801 attacactac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag 1861 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc 1921 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc 1981 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc 2041 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg 2101 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc 2161 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt 2221 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa 2281 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc 2341 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc 2401 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag 2461 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc 2521 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca 2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc 3481 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg 3541 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga 3601 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt 3661 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt 3721 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag 3781 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc 3841 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc 3901 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 3961 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 4021 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca 4081 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 4141 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt 4201 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 4261 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 4321 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 4381 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 4441 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 4501 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg 4561 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 4621 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 4681 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc 4741 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc 4801 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg 4861 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc 4921 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 4981 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 5041 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga 5101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 5161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 5221 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 5281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 5341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 5401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 5461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 5521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 5581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 5641 caaaaattta acgcgaattt t //