LOCUS       pEnCMV-Sv2c(rat)        8249 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031790
VERSION     V031790
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             775..2955
                     /label="Sv2c(NM_031593)"
                     /note="Sv2c(NM_031593)"
                     /gene="Sv2c"
     misc_feature    3025..3613
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3679..3727
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3929..4357
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4371..4700
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4767..5561
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5737..5858
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5907..5923)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5931..5947
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5955..5985)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6000..6021
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6309..6897)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7068..7928)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7929..8033)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc caccatggaa
      781 gactcctaca aggataggac ttcactgatg aagggcgcca aggacattgc caaagaggtg
      841 aagaagcaaa cagtgaagaa ggtgaaccag gcagtggacc gggcccagga tgaatacacc
      901 cagcggtcct acagccgatt ccaggatgaa gatgatgatg atgactacta cccacctgga
      961 gaaacctaca gtggggaggc caatgatgat gaaggctcaa gtgaagccac tgagggtcac
     1021 gatgaagaag acgagatcta tgaaggggaa taccagggca tccccagcac gaaccaaggg
     1081 aaggacagca tagtgtctgt aggacaaccc aaaggagatg agtacaagga ccgcagagag
     1141 ctggagtcag agaggagggc tgatgaggag gagctcgccc agcagtatga gctgataatc
     1201 caagagtgtg gccatggccg tttccagtgg gcccttttct tcgtcctggg catggctctc
     1261 atggcagacg gcgtggaggt gtttgtggtg ggctttgtgt tacccagtgc agagacagac
     1321 ctatgcatac cgaattcagg atctggatgg ctaggcagca tagtgtacct cgggatgatg
     1381 gtgggggcgt tcttctgggg aggactggca gacaaagtgg gaaggaagca gtctcttctg
     1441 atttgcatgt ccgtcaacgg attctttgcc ttcctttctt catttgtcca aggttacggc
     1501 ttctttctcc tctgtcgttt gctttcagga ttcgggattg gaggcgccat tcccactgtg
     1561 ttctcctact ttgctgaagt cctggcccgg gagaagcgcg gtgagcacct cagttggctc
     1621 tgcatgttct ggatgattgg cggtatctat gcttcagcca tggcctgggc catcatcccc
     1681 cactatgggt ggagcttcag catgggctca gcctaccagt tccacagctg gcgcgtcttc
     1741 gtcatcgtct gtgccctccc gtgcgtctcc tcggtggtgg ccctcacctt catgcccgaa
     1801 agccctcggt tcttgctgga ggtaggaaaa catgatgaag cctggatgat tctgaagcta
     1861 attcatgata ccaacatgag agcccggggc cagccagaga aggtcttcac ggtaaataaa
     1921 atcaagactc ccaagcaaat agatgagctg attgagattg agagtgacac aggaacctgg
     1981 taccggaggt gttttgttcg gatccgcaca gaactgtacg gaatttggtt gacttttatg
     2041 agatgcttca actacccggt cagggaaaac accataaagc ttacgattgt ttggttcacc
     2101 ctgtcctttg ggtactatgg actgtccgtt tggttcccag atgtcattaa acacctccag
     2161 tctgacgagt acgccctgct gactcggaat gtgcagaagg ataaatatgc aaactttagc
     2221 attaacttca ccatggaaaa ccaggtccac accggaatgg aatatgacaa tggcaggttc
     2281 ctcggagtca aattcaaatc ggtaaccttc aaggattcag tgtttaagtc ctgcaccttt
     2341 gacgatgtga cctcagtcaa cacctacttc aagaactgca cgtttattga tacccttttt
     2401 gagaacacag attttgagcc ctataaattc atagacagcg agtttcaaaa ctgctcgttt
     2461 cttcacaata agacgggatg tcagattact tttgacgacg actacagtgc ctactggatt
     2521 tactttgtca actttctcgg gacattggca gtgttaccag gaaatatcgt gtctgctctc
     2581 ctgatggaca ggatcgggcg cttaacgatg ctaggtggct ccatggtgct ctcggggatc
     2641 agctgcttct tcctgtggtt tggcaccagc gaatccatga tgataggcat gctgtgcttg
     2701 tacaacggac tgaccatctc agcgtggaac tctcttgatg tagtcacggt ggaactatac
     2761 cccacagacc ggagagcaac gggctttggc ttcttgaacg cactctgtaa agcagcggcc
     2821 gtcctgggaa acttaatatt cggctccttg gtcagcatca ccaaagcaat ccctatcctg
     2881 ctggcttcca ccgtgctcgt gtgtggagga ctcgtggggc tgcgcctgcc cgacacaaga
     2941 acccaggttc tgatgtgact cgagtctaga gggcccctta agggttcgat ccctaccggt
     3001 tagtaatgag tttgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3421 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3601 gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg
     3661 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     3721 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     3781 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     3841 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg
     3901 cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3961 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4021 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4081 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4141 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4201 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4261 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
     4321 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4381 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4441 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4501 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4561 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4621 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4681 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4741 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4801 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4861 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4921 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4981 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5041 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5101 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5161 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5221 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5281 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5341 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5401 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5461 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5521 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     5581 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5641 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5701 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5761 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5821 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5881 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5941 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6001 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6061 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6481 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     6541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6721 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7381 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7681 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8041 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga
     8101 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8161 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8221 ccgacaattg catgaagaat ctgcttagg
//
