LOCUS       V020514                10823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020514
VERSION     V020514
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..5503
                     /label="Synj1(NM_001164483)"
                     /note="Synj1(NM_001164483)"
                     /gene="Synj1"
     CDS             5510..5575
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5599..6187
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6253..6301
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6503..6931
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6945..7274
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7341..8135
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8311..8432
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8481..8497)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8505..8521
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8529..8559)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8574..8595
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8883..9471)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9642..10502)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10503..10607)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgcggaa gagctggacc tgctggagcg gaagtgacgc
      721 ttcgggcggc tgtagcggcg gaggctgcgg gagaaggagg aggaggagcc ggaggaagag
      781 ggctgcctct gaagaaagga gaatggcgtt cagcaaagga tttcgaatct accacaaatt
      841 ggatccccca ccgttcagcc tcatagtgga aactaggcat aaggaagaat gtctcatgtt
      901 cgagtctggg gctgtggctg tgctctcatc tgcagaaaag gaggctatta aaggcacata
      961 tgccaaagta ctggatgcat acggactttt gggagtttta cgattaaatc ttggtgatac
     1021 catgctgcac tatctggtcc tagtcactgg atgcatgtct gtggggaaaa tccaagaatc
     1081 tgaagttttc cgagttactt ccactgagtt tatatcattg cgagttgatg cttcagatga
     1141 ggaccgcatt tcggaagtac gaaaagtttt gaactcggga aacttttatt ttgcatggtc
     1201 tgcatctgga gtcagcttag atctgagtct gaatgcacat cgaagcatgc aagagcacac
     1261 aaccgacaat aggtttttct ggaatcagtc tctgcatttg catctcaagc actatggtgt
     1321 gaactgcgat gactggttat tacggctcat gtgtggggga gtagagatta gaaccattta
     1381 tgctgcccat aaacaggcaa aagcttgcct catctcaaga ctaagctgtg aacgagctgg
     1441 gacaaggttc aatgtccggg gaaccaacga tgatggtcat gttgccaact ttgtagagac
     1501 agagcaggtt atatacttag atgactgtgt ctcttccttc atacaaatcc gaggatctgt
     1561 tccattgttc tgggagcaac cagggttgca agtgggatcc catcgtgttc gtatgtcaag
     1621 gggatttgaa gccaatgcgc ctgcctttga caggcatttc cgaacactta aggacttata
     1681 tggtaaacag atagtagtga atctgcttgg gtctaaggaa ggcgagcata tgctgagcaa
     1741 ggctttccag agtcatttaa aagcctctga acacgcttct gatatccaca tggtgtcttt
     1801 tgactatcat caaatggtta aaggaggaaa ggcagaaaaa ctacacagta ttctcaaacc
     1861 acaagtccag aagtttctag attatggatt tttttatttc gatggcagtg aagttcaaag
     1921 gtgccagagt ggtacagttc gaacaaactg cttggattgt ctggatagaa caaatagtgt
     1981 gcaggcattc cttggcttag agatgctcgc taagcagctg gaagctctgg gcttggctga
     2041 gaagcctcag ctggtgaccc gcttccaaga agttttccgg tccatgtggt ctgtgaatgg
     2101 cgactcaatc agtaaaatat atgcaggcac cggggccctg gaagggaagg ccaagttaaa
     2161 agatggtgcg cgatctgtca ccagaaccat ccagaataac ttcttcgaca gctccaagca
     2221 ggaagccatt gatgtcctgc tcctgggaaa tactctcaac agcgatttag ctgacaaagc
     2281 ccgagcgctt ctaactactg gaagtttgcg tgtttctgaa cagacattac agtcagcatc
     2341 ttccaaagtc ttaaagaaca tgtgtgagaa cttctacaaa tactcaaagc ccaagaagat
     2401 ccgagtgtgt gttggcacct ggaatgtgaa cggcgggaag cagttccgca gcatagcgtt
     2461 caagaaccag acgctcacag actggcttct ggacgctccc aagttagccg gcatccagga
     2521 gtttcaagat aagagaagta agccaactga tatatttgca attggctttg aagaaatggt
     2581 ggagctgaat gctgggaaca ttgtgaatgc aagcacaacc aaccagaagc tgtgggcagt
     2641 ggagctgcag aagaccatct ccagggacaa caagtatgtc ctgctggcct cggagcagct
     2701 ggtgggtgtc tgcctgtttg tctttatcag accacagcac gctcctttta tcagggatgt
     2761 tgcagttgat actgtgaaaa ctggcatggg aggtgcaact ggaaacaagg gcgccgttgc
     2821 aattcggatg ctcttccaca ccaccagcct ttgcttcgtc tgcagccact ttgctgcagg
     2881 gcagtcccaa gtcaaagaga gaaatgaaga ttttgtagaa atagcacgaa aactgagttt
     2941 ccccatggga cgaatgctct tctcccatga ctatgtattt tggtgtggtg atttcaacta
     3001 tcgaattgac cttccgaatg aagaggttaa agagctcata agacaacaaa actgggattc
     3061 tctgatcgct ggggatcagc tcatcaacca gaaaaatgca ggacagatct ttagaggatt
     3121 cttagaggga aaagtaacgt ttgctccaac ctataaatat gacttgtttt ctgaagacta
     3181 tgacaccagt gagaagtgtc gcacccctgc ttggacagac cgtgtcctct ggagaaggag
     3241 gaagtggcct tttgacagat cagctgaaga tttagatctc ctgaatgcta gtttccaaga
     3301 tgaaagtaaa attctttata catggacccc tggcaccttg ctgcactatg gaagagccga
     3361 gctgaagact tctgaccata ggcccgtcgt tgccttgatt gacatagata tatttgaagt
     3421 tgaagctgaa gagagacaaa aaatttataa agaagttatt gccgtccagg gcccaccaga
     3481 tggcacagtg ctggtctcaa tcaaaagttc tgcacaagaa agtacttttt ttgatgatgc
     3541 tttgattgat gagcttctgc ggcagtttgc acactttggt gaagttatac tcataagatt
     3601 tgtagaagat aaaatgtggg ttacattttt ggagggaagc tctgccttga atgctctgag
     3661 cctaaatggg aaagagctat taaatcggac tataacaatt actttaaaaa gtccagactg
     3721 gatcaaacat ttggaagaag agatgagttt agagaaaatc agtgtcacgt tgccctcatc
     3781 agcaagctcc accctgcttg gtgaagacgc agaggtcgca gcagactttg acatggaagg
     3841 tgacgtcgat gactacagcg ctgaagtaga ggagcttctt cctcagcatc tgcagccgtc
     3901 ctcaagttct ggcctgggca cttctccaag ttcttcaccc cggaccagtc cctgccagtc
     3961 ccccacagta ccagagtact ccgccccttc tcttcccatc agacctagcc gagcaccgtc
     4021 aagaactcca ggacctccaa gttcgcaggg ctctccagtt gacactcagc cagcggccca
     4081 gaaggattct tcccagactc tagagcccaa gagaccaccc cctccccgcc cagtcgctcc
     4141 tccggcacgt cctgccccac cacagagacc acctccacct tcaggggcta ggagtcctgc
     4201 gcctgctaga aaagaatttg gaggtgttgg agcccctccc agtcccgggg ttgctaggag
     4261 agagatagaa gcacccaaaa gccctggaac agcacggaaa gataatatag ggcgtaatca
     4321 gccttcccct caagccggac ttgcaggccc aggacccgct ggatacggtg cggctagacc
     4381 gacaattcca gctcgtgctg gagtgatcag cgcccctcag agccaggctc gggtatgtgc
     4441 tggaaggccg actcctgaca gccaaagcaa gccctcggag acattgaaag gtcctgctgt
     4501 ccttccagaa ccactgaagc ctcaggctgc atttcctcag cagccttctc tgcccacacc
     4561 tgctcaaaag ttacaggacc cgcttgtccc catagcagca cctactatgc ctccctctgg
     4621 cccccaacca aatttggaaa cccccccaca gcccccacct cggagcaggt catctcaaag
     4681 cttgccttca gactcctcac cacagctgca ggtaaaaata aatggcattt ctggtgtcaa
     4741 acaagagcca acattgaaga gtgacccatt tgaagatctc tcacttagtg tgcttgctgt
     4801 atcaaaggct cagccttctg ttcagatttc acctgttcta accccagacc caaagatgtt
     4861 gattcagttg ccttctgcat cgcaaagtca agttaaccct ttgagttctg taagttgcat
     4921 gccaaccagg cctccaggtc cagaggaaag caagtcacag gagagtatgg gcagttctgc
     4981 aaacccgttt cccagcctgc cctgccggaa cccttttaca gacaggactg ctgcccctgg
     5041 aaacccattt agagttcagt cccaagaatc agaggcaact tcttggctct ccaaagaaga
     5101 gcctgttccc aacagtccct tccctcctct catgcctctc agtcatgaca cgagcaaggc
     5161 ttcaagttcg cttggtggct ttgaggacaa ttttgatctg cagagccagt ctacagtaaa
     5221 aacaagcaac cccaaaggat gggtaacctt tgacgaagac gacaactttc ctacaacagg
     5281 aaagtcaaag tcagtttgtc cagacttagt gggtaacgcg ccagcttcat ttgatgatga
     5341 ctggagtaaa ggtgcgagcg tttctttctg tgtgttgcca gccagaaggc caccaccacc
     5401 accacctcct gtccctctgc taccacctgg cacaacctca tctgcaggac ctagcacaac
     5461 cctgccatct aaggcacctt ccacattgga cttcacggaa agactcgagg actacaaaga
     5521 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtagtg
     5581 agggcccgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5641 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5701 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5761 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5821 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5881 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5941 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6001 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6061 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6121 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6181 tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     6241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     6301 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     6361 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     6421 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc
     6481 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6541 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     6661 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6841 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     6901 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7021 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7201 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7261 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     7321 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7381 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7441 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7501 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7561 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7621 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7681 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7741 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7801 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7861 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7921 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7981 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8041 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8101 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg
     8161 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     8221 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8281 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8461 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8641 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9061 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10621 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca
    10681 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
    10741 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
    10801 attgcatgaa gaatctgctt agg
//
