LOCUS       V024627                10775 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024627
VERSION     V024627
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             739..5331
                     /label="TOP2A(NM_001067)"
                     /note="TOP2A(NM_001067)"
                     /gene="TOP2A"
     CDS             5347..5388
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     CDS             5395..5418
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     CDS             5458..5481
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     misc_feature    5551..6139
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6205..6253
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6455..6883
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6897..7226
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7293..8087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8263..8384
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8433..8449)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8457..8473
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8481..8511)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8526..8547
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8835..9423)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9594..10454)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10455..10559)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagccaccat ggaagtgtca ccattgcagc ctgtaaatga aaatatgcaa
      781 gtcaacaaaa taaagaaaaa tgaagatgct aagaaaagac tgtctgttga aagaatctat
      841 caaaagaaaa cacaattgga acatattttg ctccgcccag acacctacat tggttctgtg
      901 gaattagtga cccagcaaat gtgggtttac gatgaagatg ttggcattaa ctatagggaa
      961 gtcacttttg ttcctggttt gtacaaaatc tttgatgaga ttctagttaa tgctgcggac
     1021 aacaaacaaa gggacccaaa aatgtcttgt attagagtca caattgatcc ggaaaacaat
     1081 ttaattagta tatggaataa tggaaaaggt attcctgttg ttgaacacaa agttgaaaag
     1141 atgtatgtcc cagctctcat atttggacag ctcctaactt ctagtaacta tgatgatgat
     1201 gaaaagaaag tgacaggtgg tcgaaatggc tatggagcca aattgtgtaa catattcagt
     1261 accaaattta ctgtggaaac agccagtaga gaatacaaga aaatgttcaa acagacatgg
     1321 atggataata tgggaagagc tggtgagatg gaactcaagc ccttcaatgg agaagattat
     1381 acatgtatca cctttcagcc tgatttgtct aagtttaaaa tgcaaagcct ggacaaagat
     1441 attgttgcac taatggtcag aagagcatat gatattgctg gatccaccaa agatgtcaaa
     1501 gtctttctta atggaaataa actgccagta aaaggatttc gtagttatgt ggacatgtat
     1561 ttgaaggaca agttggatga aactggtaac tccttgaaag taatacatga acaagtaaac
     1621 cacaggtggg aagtgtgttt aactatgagt gaaaaaggct ttcagcaaat tagctttgtc
     1681 aacagcattg ctacatccaa gggtggcaga catgttgatt atgtagctga tcagattgtg
     1741 actaaacttg ttgatgttgt gaagaagaag aacaagggtg gtgttgcagt aaaagcacat
     1801 caggtgaaaa atcacatgtg gatttttgta aatgccttaa ttgaaaaccc aacctttgac
     1861 tctcagacaa aagaaaacat gactttacaa cccaagagct ttggatcaac atgccaattg
     1921 agtgaaaaat ttatcaaagc tgccattggc tgtggtattg tagaaagcat actaaactgg
     1981 gtgaagttta aggcccaagt ccagttaaac aagaagtgtt cagctgtaaa acataataga
     2041 atcaagggaa ttcccaaact cgatgatgcc aatgatgcag ggggccgaaa ctccactgag
     2101 tgtacgctta tcctgactga gggagattca gccaaaactt tggctgtttc aggccttggt
     2161 gtggttggga gagacaaata tggggttttc cctcttagag gaaaaatact caatgttcga
     2221 gaagcttctc ataagcagat catggaaaat gctgagatta acaatatcat caagattgtg
     2281 ggtcttcagt acaagaaaaa ctatgaagat gaagattcat tgaagacgct tcgttatggg
     2341 aagataatga ttatgacaga tcaggaccaa gatggttccc acatcaaagg cttgctgatt
     2401 aattttatcc atcacaactg gccctctctt ctgcgacatc gttttctgga ggaatttatc
     2461 actcccattg taaaggtatc taaaaacaag caagaaatgg cattttacag ccttcctgaa
     2521 tttgaagagt ggaagagttc tactccaaat cataaaaaat ggaaagtcaa atattacaaa
     2581 ggtttgggca ccagcacatc aaaggaagct aaagaatact ttgcagatat gaaaagacat
     2641 cgtatccagt tcaaatattc tggtcctgaa gatgatgctg ctatcagcct ggcctttagc
     2701 aaaaaacaga tagatgatcg aaaggaatgg ttaactaatt tcatggagga tagaagacaa
     2761 cgaaagttac ttgggcttcc tgaggattac ttgtatggac aaactaccac atatctgaca
     2821 tataatgact tcatcaacaa ggaacttatc ttgttctcaa attctgataa cgagagatct
     2881 atcccttcta tggtggatgg tttgaaacca ggtcagagaa aggttttgtt tacttgcttc
     2941 aaacggaatg acaagcgaga agtaaaggtt gcccaattag ctggatcagt ggctgaaatg
     3001 tcttcttatc atcatggtga gatgtcacta atgatgacca ttatcaattt ggctcagaat
     3061 tttgtgggta gcaataatct aaacctcttg cagcccattg gtcagtttgg taccaggcta
     3121 catggtggca aggattctgc tagtccacga tacatcttta caatgctcag ctctttggct
     3181 cgattgttat ttccaccaaa agatgatcac acgttgaagt ttttatatga tgacaaccag
     3241 cgtgttgagc ctgaatggta cattcctatt attcccatgg tgctgataaa tggtgctgaa
     3301 ggaatcggta ctgggtggtc ctgcaaaatc cccaactttg atgtgcgtga aattgtaaat
     3361 aacatcaggc gtttgatgga tggagaagaa cctttgccaa tgcttccaag ttacaagaac
     3421 ttcaagggta ctattgaaga actggctcca aatcaatatg tgattagtgg tgaagtagct
     3481 attcttaatt ctacaaccat tgaaatctca gagcttcccg tcagaacatg gacccagaca
     3541 tacaaagaac aagttctaga acccatgttg aatggcaccg agaagacacc tcctctcata
     3601 acagactata gggaatacca tacagatacc actgtgaaat ttgttgtgaa gatgactgaa
     3661 gaaaaactgg cagaggcaga gagagttgga ctacacaaag tcttcaaact ccaaactagt
     3721 ctcacatgca actctatggt gctttttgac cacgtaggct gtttaaagaa atatgacacg
     3781 gtgttggata ttctaagaga cttttttgaa ctcagactta aatattatgg attaagaaaa
     3841 gaatggctcc taggaatgct tggtgctgaa tctgctaaac tgaataatca ggctcgcttt
     3901 atcttagaga aaatagatgg caaaataatc attgaaaata agcctaagaa agaattaatt
     3961 aaagttctga ttcagagggg atatgattcg gatcctgtga aggcctggaa agaagcccag
     4021 caaaaggttc cagatgaaga agaaaatgaa gagagtgaca acgaaaagga aactgaaaag
     4081 agtgactccg taacagattc tggaccaacc ttcaactatc ttcttgatat gcccctttgg
     4141 tatttaacca aggaaaagaa agatgaactc tgcaggctaa gaaatgaaaa agaacaagag
     4201 ctggacacat taaaaagaaa gagtccatca gatttgtgga aagaagactt ggctacattt
     4261 attgaagaat tggaggctgt tgaagccaag gaaaaacaag atgaacaagt cggacttcct
     4321 gggaaagggg ggaaggccaa ggggaaaaaa acacaaatgg ctgaagtttt gccttctccg
     4381 cgtggtcaaa gagtcattcc acgaataacc atagaaatga aagcagaggc agaaaagaaa
     4441 aataaaaaga aaattaagaa tgaaaatact gaaggaagcc ctcaagaaga tggtgtggaa
     4501 ctagaaggcc taaaacaaag attagaaaag aaacagaaaa gagaaccagg tacaaagaca
     4561 aagaaacaaa ctacattggc atttaagcca atcaaaaaag gaaagaagag aaatccctgg
     4621 tctgattcag aatcagatag gagcagtgac gaaagtaatt ttgatgtccc tccacgagaa
     4681 acagagccac ggagagcagc aacaaaaaca aaattcacaa tggatttgga ttcagatgaa
     4741 gatttctcag attttgatga aaaaactgat gatgaagatt ttgtcccatc agatgctagt
     4801 ccacctaaga ccaaaacttc cccaaaactt agtaacaaag aactgaaacc acagaaaagt
     4861 gtcgtgtcag accttgaagc tgatgatgtt aagggcagtg taccactgtc ttcaagccct
     4921 cctgctacac atttcccaga tgaaactgaa attacaaacc cagttcctaa aaagaatgtg
     4981 acagtgaaga agacagcagc aaaaagtcag tcttccacct ccactaccgg tgccaaaaaa
     5041 agggctgccc caaaaggaac taaaagggat ccagctttga attctggtgt ctctcaaaag
     5101 cctgatcctg ccaaaaccaa gaatcgccgc aaaaggaagc catccacttc tgatgattct
     5161 gactctaatt ttgagaaaat tgtttcgaaa gcagtcacaa gcaagaaatc caagggggag
     5221 agtgatgact tccatatgga ctttgactca gctgtggctc ctcgggcaaa atctgtacgg
     5281 gcaaagaaac ctataaagta cctggaagag tcagatgaag atgatctgtt tgcggccgcg
     5341 ggtagcggta agcctatccc taaccctctc ctcggtctcg attctacggg tagctggagc
     5401 catccgcagt ttgaaaaagg tggtggtagc ggtggtggtt caggtggtag cagtgcatgg
     5461 tcacaccctc agtttgagaa atgactcgag tctagagggc cccttaaggg ttcgatccct
     5521 accggttagt aatgagtttg atatctcgac aatcaacctc tggattacaa aatttgtgaa
     5581 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5641 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5701 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5761 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5821 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5881 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5941 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6001 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6061 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6121 ctttgggccg cctccccgcc tggaaacggg ggaggctaac tgaaacacgg aaggagacaa
     6181 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     6241 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6301 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6361 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca
     6421 gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6601 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     6841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7141 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7201 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7261 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7321 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7381 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7441 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7501 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7561 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7621 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7681 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7741 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7801 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7861 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7921 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7981 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8041 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8101 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8161 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8221 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8401 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8461 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8521 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9001 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     9061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9241 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10561 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10621 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10681 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10741 gcttgaccga caattgcatg aagaatctgc ttagg
//
