LOCUS       V031191                 7455 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031191
VERSION     V031191
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3602
                     /label="Tas1r3(NM_031872)"
                     /note="Tas1r3(NM_031872)"
                     /gene="Tas1r3"
     CDS             3621..3650
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3669..3692
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3712..3728)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3739..4361
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4510..5098)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5427..5474
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5706..6500)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6535..6892)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6894..6998)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7025..7453
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gccagctttg gctatcatgg gtctcagcct ggctgctttc ctggagcttg
     1081 ggatgggggc ctctttgtgt ctgtcacagc aattcaaggc acaaggggac tacatactgg
     1141 gcgggctatt tcccctgggc tcaaccgagg aggccactct caaccagaga acacaaccca
     1201 acagcatccc gtgcaacagg ttctcacccc ttggtttgtt cctggccatg gctatgaaga
     1261 tggctgtgga ggagatcaac aatggatctg ccttgctccc tgggctgcgg ctgggctatg
     1321 acctatttga cacatgctcc gagccagtgg tcaccatgaa atccagtctc atgttcctgg
     1381 ccaaggtggg cagtcaaagc attgctgcct actgcaacta cacacagtac caaccccgtg
     1441 tgctggctgt catcggcccc cactcatcag agcttgccct cattacaggc aagttcttca
     1501 gcttcttcct catgccacag gtcagctata gtgccagcat ggatcggcta agtgaccggg
     1561 aaacgtttcc atccttcttc cgcacagtgc ccagtgaccg ggtgcagctg caggcagttg
     1621 tgactctgtt gcagaacttc agctggaact gggtggccgc cttagggagt gatgatgact
     1681 atggccggga aggtctgagc atcttttcta gtctggccaa tgcacgaggt atctgcatcg
     1741 cacatgaggg cctggtgcca caacatgaca ctagtggcca acagttgggc aaggtgctgg
     1801 atgtactacg ccaagtgaac caaagtaaag tacaagtggt ggtgctgttt gcctctgccc
     1861 gtgctgtcta ctcccttttt agttacagca tccatcatgg cctctcaccc aaggtatggg
     1921 tggccagtga gtcttggctg acatctgacc tggtcatgac acttcccaat attgcccgtg
     1981 tgggcactgt gcttgggttt ttgcagcggg gtgccctact gcctgaattt tcccattatg
     2041 tggagactca ccttgccctg gccgctgacc cagcattctg tgcctcactg aatgcggagt
     2101 tggatctgga ggaacatgtg atggggcaac gctgtccacg gtgtgacgac atcatgctgc
     2161 agaacctatc atctgggctg ttgcagaacc tatcagctgg gcaattgcac caccaaatat
     2221 ttgcaaccta tgcagctgtg tacagtgtgg ctcaagccct tcacaacacc ctacagtgca
     2281 atgtctcaca ttgccacgta tcagaacatg ttctaccctg gcagctcctg gagaacatgt
     2341 acaatatgag tttccatgct cgagacttga cactacagtt tgatgctgaa gggaatgtag
     2401 acatggaata tgacctgaag atgtgggtgt ggcagagccc tacacctgta ttacatactg
     2461 tgggcacctt caacggcacc cttcagctgc agcagtctaa aatgtactgg ccaggcaacc
     2521 aggtgccagt ctcccagtgt tcccgccagt gcaaagatgg ccaggttcgc cgagtaaagg
     2581 gctttcattc ctgctgctat gactgcgtgg actgcaaggc gggcagctac cggaagcatc
     2641 cagatgactt cacctgtact ccatgtaacc aggaccagtg gtccccagag aaaagcacag
     2701 cctgcttacc tcgcaggccc aagtttctgg cttgggggga gccagttgtg ctgtcactcc
     2761 tcctgctgct ttgcctggtg ctgggtctag cactggctgc tctggggctc tctgtccacc
     2821 actgggacag ccctcttgtc caggcctcag gtggctcaca gttctgcttt ggcctgatct
     2881 gcctaggcct cttctgcctc agtgtccttc tgttcccagg gcggccaagc tctgccagct
     2941 gccttgcaca acaaccaatg gctcacctcc ctctcacagg ctgcctgagc acactcttcc
     3001 tgcaagcagc tgagaccttt gtggagtctg agctgccact gagctgggca aactggctat
     3061 gcagctacct tcggggactc tgggcctggc tagtggtact gttggccact tttgtggagg
     3121 cagcactatg tgcctggtat ttgatcgctt tcccaccaga ggtggtgaca gactggtcag
     3181 tgctgcccac agaggtactg gagcactgcc acgtgcgttc ctgggtcagc ctgggcttgg
     3241 tgcacatcac caatgcaatg ttagctttcc tctgctttct gggcactttc ctggtacaga
     3301 gccagcctgg ccgctacaac cgtgcccgtg gtctcacctt cgccatgcta gcttatttca
     3361 tcacctgggt ctcttttgtg cccctcctgg ccaatgtgca ggtggcctac cagccagctg
     3421 tgcagatggg tgctatccta gtctgtgccc tgggcatcct ggtcaccttc cacctgccca
     3481 agtgctatgt gcttctttgg ctgccaaagc tcaacaccca ggagttcttc ctgggaagga
     3541 atgccaagaa agcagcagat gagaacagtg gcggtggtga ggcagctcag ggacacaatg
     3601 aaacgcgtac gcggccgctc gagcagaaac tcatctcaga agaggatctg gcagcaaatg
     3661 atatcctgga ttacaaggat gacgacgata aggtttaaac ggccggccgc ggtcatagct
     3721 gtttcctgaa cagatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3781 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3841 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3901 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3961 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     4021 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     4081 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     4141 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     4201 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag
     4261 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc
     4321 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcggcg
     4381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4921 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5221 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     5281 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     5341 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     5401 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     5461 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     5521 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     5581 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     5641 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     5701 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     5761 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     5821 tcacgggtag ccaacgctat gtcctgatag cgatccgcca cacccagccg gccacagtcg
     5881 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     5941 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     6001 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     6061 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     6121 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     6181 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     6241 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     6301 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     6361 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     6421 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     6481 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     6541 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     6601 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     6661 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     6721 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     6781 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     6841 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     6901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7021 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7441 tttaacgcga atttt
//
