LOCUS       V031112                 6216 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031112
VERSION     V031112
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2363
                     /label="Tbx20(NM_194263)"
                     /note="Tbx20(NM_194263)"
                     /gene="Tbx20"
     CDS             2382..2411
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2430..2453
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2473..2489)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2500..3122
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3271..3859)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4188..4235
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4467..5261)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5296..5653)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5655..5759)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5786..6214
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagttcacg gcgtcgccca agccccagct ctcctctcga gccaatgcct
     1081 tctccatcgc cgcgcttatg tccagcggcg gccccaagga gaaggaggca gcagagaaca
     1141 ccatcaaacc cctggaacaa tttgtggaga aatcatcatg tgcccagcca ctgggtgagc
     1201 tgacgagtct ggatgctcac gcggagtttg gcggcggggg cggcagccca tcctcatcct
     1261 ctctgtgcac agagccactg atacccacca cccccatcat ccccagcgaa gagatggcta
     1321 aaatcgcctg cagcctggaa acgaaggagc tctgggacaa attccatgaa ctgggcacgg
     1381 agatgataat caccaagtct ggcaggagga tgttccccac catccgcgtg tcattttctg
     1441 gagtggatcc tgagtccaag tatatagtcc tgatggacat cgtcccggtg gacaacaaga
     1501 gataccgcta tgcctaccac cggtcatcct ggctggtggc tggcaaagct gatcccccgc
     1561 tgccagccag gctctacgtg cacccagact ccccctttac tggcgagcag ctcctcaaac
     1621 agatggtgtc ttttgaaaag gtgaagctca ccaacaatga actggatcaa cacggccata
     1681 taattttgaa ttcaatgcat aagtaccagc cacgggtgca catcataaag aagaaagacc
     1741 acacggcctc cttgctcaat ctgaagtcag aagaattcag gacgttcatc tttccagaga
     1801 cagttttcac agcagtcaca gcctaccaga accaactgat aaccaagctg aaaatagaca
     1861 gcaatccgtt tgccaaagga ttccgggact cctccaggct cactgacatt gagagggaga
     1921 gtgtggagag cctgatccag aagcattcct atgcccggtc acccatccgc acctatgggg
     1981 aagaggatgt tctgggggag gagagtcaaa caactcagag tcgaggatca gcctttacaa
     2041 catctgacaa tttgtctctc agttcctggg tatcgtcatc ttccagtttt cctggatttc
     2101 agcatccaca gcccctgact gctcttggta ccagtacagc atccatagcg acaccgattc
     2161 ctcaccctat acagggttct ctgccaccat atagccgcct gggaatgcct ctgaccccat
     2221 ctgcaatagc cagctccatg cagggaagtg gtcccacgtt cccttcattc cacatgccta
     2281 gataccatca ctacttccag caggggccct acgctgccat ccaaggactt cgccactcct
     2341 ccgctgtgat gacgccattt gtaacgcgta cgcggccgct cgagcagaaa ctcatctcag
     2401 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     2461 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     2521 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2581 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2641 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2701 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     2761 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     2821 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     2881 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     2941 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     3001 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     3061 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     3121 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     4021 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     4081 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     4141 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     4201 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4261 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4321 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4381 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4441 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4501 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4561 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4621 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4681 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4741 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4801 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4861 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4921 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4981 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5041 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5101 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5161 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5221 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5281 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5341 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5401 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5461 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5521 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5581 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5641 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5701 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5761 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5821 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6001 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6121 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6181 aatgagctga tttaacaaaa atttaacgcg aatttt
//
