LOCUS V031218 6771 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031218 VERSION V031218 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2918 /label="Tec(NM_001113460)" /note="Tec(NM_001113460)" /gene="Tec" CDS 2937..2966 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2985..3008 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(3028..3044) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 3055..3677 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3826..4414) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4743..4790 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(5022..5816) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5851..6208) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(6210..6314) /gene="bla" /label="AmpR promoter" rep_origin 6341..6769 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaatttcaac actatcctag aagagattct tattaaaagg tcccagcaga 1081 aaaagaagac atcaccctta aactacaaag agagactttt tgtacttaca aaatccgtgt 1141 tgagctacta tgagggtcga gcggagaaga aatacagaaa gggcgtcatt gatatttcca 1201 aaatcaagtg tgtggagata gtgaagaacg atgatggtgt cattccctgt caaaataaat 1261 ttccattcca ggttgttcat gatgctaata cactttatat ttttgcacct agtccacaaa 1321 gcagggaccg atgggtgaag aagttaaaag aagaaataaa gaacaacaat aatatcatga 1381 ttaaatacca tcctaaattc tgggcagatg ggagttacca gtgttgtaga caaacagaaa 1441 aactagcacc cggatgtgag aagtacaatc tttttgagag tagtataaga aagaccctgc 1501 ctcccgcgcc agaaataaag aagagaaggc ctcctccacc aattccccca gaggaagaaa 1561 atactgaaga aatcgttgta gcgatgtatg acttccaagc gacggaagca catgacctca 1621 ggttagagag aggccaagag tatatcatcc tggaaaagaa tgacctccat tggtggagag 1681 cgagagataa gtatgggagt gaaggatata tcccaagtaa ttacgtcaca gggaagaaat 1741 ccaacaactt agatcaatat gagtggtact gcagaaatac caacagaagc aaagcagaac 1801 agctcctcag aacggaagat aaagaaggtg gttttatggt gagagactcc agtcaaccag 1861 gcttgtacac tgtctccctt tacacaaagt ttgggggaga aggctcatca ggtttcaggc 1921 attatcacat aaaggaaaca gcaacatccc caaagaagta ttacctggca gagaagcatg 1981 ctttcgggtc cattcctgag atcattgaat atcacaagca caatgcggca gggcttgtca 2041 ccaggctgcg gtacccggtc agtacaaagg ggaagaacgc tcccactact gccggcttca 2101 gctatgataa gtgggagatt aacccatcag agctgacctt tatgagagag ttggggagcg 2161 gactgtttgg agtggtgagg cttggcaagt ggcgggccca gtacaaagtg gccatcaaag 2221 ctatccggga aggcgccatg tgtgaagagg atttcataga ggaagctaaa gtcatgatga 2281 agctgacaca ccccaagctg gtacagctct atggtgtatg cacccagcag aagcccatct 2341 acatcgttac cgagttcatg gaacggggct gccttctgaa tttcctccgg cagagacaag 2401 gccatttcag cagagacatg ctgctaagca tgtgtcaaga tgtctgtgaa gggatggagt 2461 acctggagag aaacagcttc atccacagag acctggctgc cagaaattgt ctagtgaatg 2521 aagcaggagt tgtcaaagta tctgattttg gaatggccag gtacgttctg gatgatcagt 2581 acacaagttc ttctggcgcc aagttccctg tgaagtggtg tcccccagaa gtgtttaatt 2641 acagccgctt tagcagcaag tcagacgtct ggtcgtttgg tgtgctaatg tgggaaatat 2701 tcacagaagg caggatgccc tttgagaaga acaccaatta cgaagtggta accatggtga 2761 ctcgtggcca ccgcctccac cggccaaagc tggcttccaa atatttgtat gaggtgatgc 2821 tgagatgctg gcaagagaga ccagagggaa ggccttcctt tgaagacttg ctgcgtacga 2881 tagatgaact agttgaatgt gaagaaactt ttggaagaac gcgtacgcgg ccgctcgagc 2941 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg 3001 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg 3061 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc 3121 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat 3181 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt 3241 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact 3301 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga 3361 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc 3421 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct 3481 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta 3541 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa 3601 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag 3661 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc 3721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4561 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg 4621 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc 4681 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt 4741 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg 4801 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa 4861 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 4921 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 4981 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 5041 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 5101 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 5161 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 5221 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 5281 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 5341 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 5401 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 5461 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 5521 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 5581 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 5641 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 5701 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 5761 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 5821 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc 5881 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 5941 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 6001 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 6061 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 6121 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 6181 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt 6241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 6301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 6361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 6481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 6541 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 6601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 6661 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 6721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t //