LOCUS       V031218                 6771 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031218
VERSION     V031218
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2918
                     /label="Tec(NM_001113460)"
                     /note="Tec(NM_001113460)"
                     /gene="Tec"
     CDS             2937..2966
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2985..3008
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3028..3044)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3055..3677
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3826..4414)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4743..4790
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5022..5816)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5851..6208)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6210..6314)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6341..6769
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaatttcaac actatcctag aagagattct tattaaaagg tcccagcaga
     1081 aaaagaagac atcaccctta aactacaaag agagactttt tgtacttaca aaatccgtgt
     1141 tgagctacta tgagggtcga gcggagaaga aatacagaaa gggcgtcatt gatatttcca
     1201 aaatcaagtg tgtggagata gtgaagaacg atgatggtgt cattccctgt caaaataaat
     1261 ttccattcca ggttgttcat gatgctaata cactttatat ttttgcacct agtccacaaa
     1321 gcagggaccg atgggtgaag aagttaaaag aagaaataaa gaacaacaat aatatcatga
     1381 ttaaatacca tcctaaattc tgggcagatg ggagttacca gtgttgtaga caaacagaaa
     1441 aactagcacc cggatgtgag aagtacaatc tttttgagag tagtataaga aagaccctgc
     1501 ctcccgcgcc agaaataaag aagagaaggc ctcctccacc aattccccca gaggaagaaa
     1561 atactgaaga aatcgttgta gcgatgtatg acttccaagc gacggaagca catgacctca
     1621 ggttagagag aggccaagag tatatcatcc tggaaaagaa tgacctccat tggtggagag
     1681 cgagagataa gtatgggagt gaaggatata tcccaagtaa ttacgtcaca gggaagaaat
     1741 ccaacaactt agatcaatat gagtggtact gcagaaatac caacagaagc aaagcagaac
     1801 agctcctcag aacggaagat aaagaaggtg gttttatggt gagagactcc agtcaaccag
     1861 gcttgtacac tgtctccctt tacacaaagt ttgggggaga aggctcatca ggtttcaggc
     1921 attatcacat aaaggaaaca gcaacatccc caaagaagta ttacctggca gagaagcatg
     1981 ctttcgggtc cattcctgag atcattgaat atcacaagca caatgcggca gggcttgtca
     2041 ccaggctgcg gtacccggtc agtacaaagg ggaagaacgc tcccactact gccggcttca
     2101 gctatgataa gtgggagatt aacccatcag agctgacctt tatgagagag ttggggagcg
     2161 gactgtttgg agtggtgagg cttggcaagt ggcgggccca gtacaaagtg gccatcaaag
     2221 ctatccggga aggcgccatg tgtgaagagg atttcataga ggaagctaaa gtcatgatga
     2281 agctgacaca ccccaagctg gtacagctct atggtgtatg cacccagcag aagcccatct
     2341 acatcgttac cgagttcatg gaacggggct gccttctgaa tttcctccgg cagagacaag
     2401 gccatttcag cagagacatg ctgctaagca tgtgtcaaga tgtctgtgaa gggatggagt
     2461 acctggagag aaacagcttc atccacagag acctggctgc cagaaattgt ctagtgaatg
     2521 aagcaggagt tgtcaaagta tctgattttg gaatggccag gtacgttctg gatgatcagt
     2581 acacaagttc ttctggcgcc aagttccctg tgaagtggtg tcccccagaa gtgtttaatt
     2641 acagccgctt tagcagcaag tcagacgtct ggtcgtttgg tgtgctaatg tgggaaatat
     2701 tcacagaagg caggatgccc tttgagaaga acaccaatta cgaagtggta accatggtga
     2761 ctcgtggcca ccgcctccac cggccaaagc tggcttccaa atatttgtat gaggtgatgc
     2821 tgagatgctg gcaagagaga ccagagggaa ggccttcctt tgaagacttg ctgcgtacga
     2881 tagatgaact agttgaatgt gaagaaactt ttggaagaac gcgtacgcgg ccgctcgagc
     2941 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     3001 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     3061 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3121 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     3181 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     3241 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     3301 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     3361 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     3421 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     3481 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     3541 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     3601 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     3661 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     3721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4561 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     4621 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     4681 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     4741 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     4801 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     4861 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     4921 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     4981 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     5041 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     5101 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     5161 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     5221 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     5281 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     5341 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     5401 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     5461 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     5521 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     5581 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     5641 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     5701 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5761 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     5821 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     5881 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5941 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     6001 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     6061 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     6121 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     6181 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     6241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6541 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6661 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
