LOCUS V031268 8247 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031268 VERSION V031268 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4394 /label="Tert(NM_009354)" /note="Tert(NM_009354)" /gene="Tert" CDS 4413..4442 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4461..4484 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4504..4520) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4531..5153 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5302..5890) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6219..6266 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6498..7292) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7327..7684) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7686..7790) /gene="bla" /label="AmpR promoter" rep_origin 7817..8245 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gacccgcgct cctcgttgcc ccgcggtgcg ctctctgctg cgcagccgat 1081 accgggaggt gtggccgctg gcaacctttg tgcggcgcct ggggcccgag ggcaggcggc 1141 ttgtgcaacc cggggacccg aagatctacc gcactttggt tgcccaatgc ctagtgtgca 1201 tgcactgggg ctcacagcct ccacctgccg acctttcctt ccaccaggtg tcatccctga 1261 aagagctggt ggccagggtt gtgcagagac tctgcgagcg caacgagaga aacgtgctgg 1321 cttttggctt tgagctgctt aacgaggcca gaggcgggcc tcccatggcc ttcactagta 1381 gcgtgcgtag ctacttgccc aacactgtta ttgagaccct gcgtgtcagt ggtgcatgga 1441 tgctactgtt gagccgagtg ggcgacgacc tgctggtcta cctgctggca cactgtgctc 1501 tttatcttct ggtgcccccc agctgtgcct accaggtgtg tgggtctccc ctgtaccaaa 1561 tttgtgccac cacggatatc tggccctctg tgtccgctag ttacaggccc acccgacccg 1621 tgggcaggaa tttcactaac cttaggttct tacaacagat caagagcagt agtcgccagg 1681 aagcaccgaa acccctggcc ttgccatctc gaggtacaaa gaggcatctg agtctcacca 1741 gtacaagtgt gccttcagct aagaaggcca gatgctatcc tgtcccgaga gtggaggagg 1801 gaccccacag gcaggtgcta ccaaccccat caggcaaatc atgggtgcca agtcctgctc 1861 ggtcccccga ggtgcctact gcagagaaag atttgtcttc taaaggaaag gtgtctgacc 1921 tgagtctctc tgggtcggtg tgctgtaaac acaagcccag ctccacatct ctgctgtcac 1981 caccccgcca aaatgccttt cagctcaggc catttattga gaccagacat ttcctttact 2041 ccaggggaga tggccaagag cgtctaaacc cctcattcct actcagcaac ctccagccta 2101 acttgactgg ggccaggaga ctggtggaga tcatctttct gggctcaagg cctaggacat 2161 caggaccact ctgcaggaca caccgtctat cgcgtcgata ctggcagatg cggcccctgt 2221 tccaacagct gctggtgaac catgcagagt gccaatatgt cagactcctc aggtcacatt 2281 gcaggtttcg aacagcaaac caacaggtga cagatgcctt gaacaccagc ccaccgcacc 2341 tcatggattt gctccgcctg cacagcagtc cctggcaggt atatggtttt cttcgggcct 2401 gtctctgcaa ggtggtgtct gctagtctct ggggtaccag gcacaatgag cgccgcttct 2461 ttaagaactt aaagaagttc atctcgttgg ggaaatacgg caagctatca ctgcaggaac 2521 tgatgtggaa gatgaaagta gaggattgcc actggctccg cagcagcccg gggaaggacc 2581 gtgtccccgc tgcagagcac cgtctgaggg agaggatcct ggctacgttc ctgttctggc 2641 tgatggacac atacgtggta cagctgctta ggtcattctt ttacatcaca gagagcacat 2701 tccagaagaa caggctcttc ttctaccgta agagtgtgtg gagcaagctg cagagcattg 2761 gagtcaggca acaccttgag agagtgcggc tacgggagct gtcacaagag gaggtcaggc 2821 atcaccagga cacctggcta gccatgccca tctgcagact gcgcttcatc cccaagccca 2881 acggcctgcg gcccattgtg aacatgagtt atagcatggg taccagagct ttgggcagaa 2941 ggaagcaggc ccagcatttc acccagcgtc tcaagactct cttcagcatg ctcaactatg 3001 agcggacaaa acatcctcac cttatggggt cttctgtact gggtatgaat gacatctaca 3061 ggacctggcg ggcctttgtg ctgcgtgtgc gtgctctgga ccagacaccc aggatgtact 3121 ttgttaaggc agatgtgacc ggggcctatg atgccatccc ccagggtaag ctggtggagg 3181 ttgttgccaa tatgatcagg cactcggaga gcacgtactg tatccgccag tatgcagtgg 3241 tccggagaga tagccaaggc caagtccaca agtcctttag gagacaggtc accaccctct 3301 ctgacctcca gccatacatg ggccagttcc ttaagcatct gcaggattca gatgccagtg 3361 cactgaggaa ctccgttgtc atcgagcaga gcatctctat gaatgagagc agcagcagcc 3421 tgtttgactt cttcctgcac ttcctgcgtc acagtgtcgt aaagattggt gacaggtgct 3481 atacgcagtg ccagggcatc ccccagggct ccagcctatc caccctgctc tgcagtctgt 3541 gtttcggaga catggagaac aagctgtttg ctgaggtgca gcgggatggg ttgcttttac 3601 gttttgttga tgactttctg ttggtgacgc ctcacttgga ccaagcaaaa accttcctca 3661 gcaccctggt ccatggcgtt cctgagtatg ggtgcatgat aaacttgcag aagacagtgg 3721 tgaacttccc tgtggagcct ggtaccctgg gtggtgcagc tccataccag ctgcctgctc 3781 actgcctgtt tccctggtgt ggcttgctgc tggacactca gactttggag gtgttctgtg 3841 actactcagg ttatgcccag acctcaatta agacgagcct caccttccag agtgtcttca 3901 aagctgggaa gaccatgcgg aacaagctcc tgtcggtctt gcggttgaag tgtcacggtc 3961 tatttctaga cttgcaggtg aacagcctcc agacagtctg catcaatata tacaagatct 4021 tcctgcttca ggcctacagg ttccatgcat gtgtgattca gcttcccttt gaccagcgtg 4081 ttaggaagaa cctcacattc tttctgggca tcatctccag ccaagcatcc tgctgctatg 4141 ctatcctgaa ggtcaagaat ccaggaatga cactaaaggc ctctggctcc tttcctcctg 4201 aagccgcaca ttggctctgc taccaggcct tcctgctcaa gctggctgct cattctgtca 4261 tctacaaatg tctcctggga cctctgagga cagcccaaaa actgctgtgc cggaagctcc 4321 cagaggcgac aatgaccatc cttaaagctg cagctgaccc agccctaagc acagactttc 4381 agaccatttt ggacacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 4441 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 4501 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 4561 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 4621 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 4681 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 4741 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 4801 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 4861 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 4921 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 4981 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 5041 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 5101 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 5161 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 6001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 6061 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 6121 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 6181 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 6241 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 6301 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 6361 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 6421 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 6481 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 6541 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 6601 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 6661 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 6721 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 6781 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 6841 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 6901 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 6961 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 7021 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 7081 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 7141 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 7201 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 7261 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 7321 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 7381 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 7441 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 7501 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 7561 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 7621 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 7681 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7801 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 7861 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 7921 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 7981 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 8041 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8101 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 8161 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 8221 atttaacaaa aatttaacgc gaatttt //