LOCUS       V046164                11835 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046164
VERSION     V046164
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6515
                     /label="Tet2(NM_001427557)"
                     /note="Tet2(NM_001427557)"
                     /gene="Tet2"
     CDS             6522..6587
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6611..7199
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7265..7313
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7515..7943
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7957..8286
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8353..9147
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9323..9444
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9493..9509)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9517..9533
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9541..9571)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9586..9607
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9895..10483)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10654..11514)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11515..11619)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga acaggacaga accacccatg
      781 ctgagggcac caggcagagt ccattcctga tagcatcacc ttctcccgtc agccatacag
      841 aacctctggc tgtcaaactc cagaacggaa gcccattagc agagagacct cacccagaag
      901 taaatgggga caccaagtgg cagtctttcc aaagctatta tggaataccc cacatgaaag
      961 gcagccagag cagtcatgag agtccagact tcatacatga aggcagaggg tattccgggt
     1021 gcttacaaaa cggagggata aaacgcacag tcagtgaacc atctctctct gggctccatc
     1081 tgaacacaaa actgacacta gaccaaaaag ctaagggaga aagtaataac ttcggggaaa
     1141 gtcaagaagg aaacccaggt aaaagcagca gccacccaaa tgtctccggt ctgagtgatg
     1201 acagagagcc cgggacctct acagcccagg aaagcacaga catagatgct ttcccaacac
     1261 ggaactacaa tggagttgaa aacacagaaa ttcccgagga gcaggaaggg ggaaatgaca
     1321 ggaacgctct attacttaaa aacaaggctg tgctaatgcc taatggtgct acagtttctg
     1381 ccccttctgt ggaagacaca catggtgaat tcctggaaaa aacacagtgt tatccagatt
     1441 gtgtttccat ggccgtgcag aataatacca cacctcacgt aaacgccctt agcggtcagg
     1501 ctgctatcga gctgcctcgt gagatcactc aaccatcgct tacctcagca cagatcaatt
     1561 tctcgcagac ctcaagcgcc cagctgcctc cagagccagc tgcaatggtg actaaggcct
     1621 gtgatttgga tagtgccagt aaaccagctg gagtactagg tacctgtgct tttcagaaag
     1681 cagaacacca acaaaagtca gttttgggaa taggcccatc atcttgtgca gaaaacataa
     1741 acatccaagg gaacacgaag ccgtttgctg aagaatacta tgctggttcc gacagtgatt
     1801 tgcaaacttc tcttggcagc tccgaacagt gcataaagca aaaggaaacc aatggtgctt
     1861 acttccggca aagctcagtg ttccctgaag attccatttc tgccactacc atgacgccac
     1921 catcaccatc tcctcttgct ccccgtcccg ttcttcagct tccttcagaa ggaaaaggca
     1981 ctctgaatga cgtagctttg gaagaacacc accgctaccc caaccaaagt gacctaactc
     2041 tttcaaggga agggaaaaca gaacaccaac ctaagaagac aacatctagc cagagtctga
     2101 acacatctgt acatacaccc aaccccccct tgatgcttcc agaacagcat cagaatgact
     2161 gcgtcccacc gtgctctgaa aaaacaagga aagtgtcaga ttatcgcatg tattacctgc
     2221 caaatcatgg ccacggtgga gatttacaag aaaatagcca atatctgatg ggacacgggg
     2281 agcacgagat tctgaaggat gcaaacaagg ggcaaacgca aggttctgtc ctggcagcgc
     2341 ctggctggat agaactgaag gcatcacatt ttcacaaagc gctccatcag acagaatgca
     2401 aggctacgtc cttgcactct ggccttcgct atcagaccag ccctaccaat cagataggct
     2461 ccatacagtc cactggaagt gtaaacatga caggagaatt ccaaaggcca ccttacatcc
     2521 agaaaacggc tcagccagag cataagtccc aaatgtacca agtggaaatg aatcaaggac
     2581 cctctccggg tatgggggac caacatctac agttccagaa aacttcctac caggagtgca
     2641 tctccaggac agatctgccc cctgaggctc acgggcctca gtatcatttc catcaaagag
     2701 tagatccctc caccgacaag caacaggcca cagaggctga actattttcc agctttttac
     2761 aacatacccc tcataagcag gcagcacaaa cacaagcatc ccagaactca aatttccctc
     2821 aagtctgcca gcagcagcat cagttacaga gaaagaataa agagcagatg cctcagactt
     2881 tctctcacct ccacggtacc aaccatgagc aaagtcaggg ccagtgtttt ggcacgatta
     2941 aagcggaaga aagcttttgt ggcgaaaatc agtactcaaa accgagtaat ttccaaactc
     3001 acagtgccca gggggggttg gagcaagtac agaatgtaaa tagaagtttt ccttatgcga
     3061 agatcgaaac accaaactca agcaaagtac aaactctctg ttcaaattat acacacccgg
     3121 cttccgagag cagagaacaa aatctacatc ttgatctctt cataggaaat aggaccccaa
     3181 accagcagaa cacgcagtgt ttcccgaata atgtgacccc aagtcaggac gtcgctcaca
     3241 ggtgctttca agaacaggag cagaagcctc accaagcttc atctctacag ggtcttaaag
     3301 gcaggagccg ggatgagttc tcagtgcctc cggctgaagc aactcaagcg aggtatttgg
     3361 tgcatattca agcaaaggca ctccctgtgc ccaagcaagg aggaagtcac acacagatct
     3421 cccctcagaa ggacacccag aagcatgctg ccttaaggag gcttctcttg cagaagcagg
     3481 aacagcagca aatacagcaa tcccagtctg agtccggcca tcatcagatg cccaggccaa
     3541 tcaagattga gcctggatcc aaactctctt cctgtaaaca ccccacgtcg gggccacaag
     3601 aaaatatgcc caaaaggata aagcaagaga gccccttgct gagctgtgat ggtgggcgga
     3661 cggaaagcat catcgagacg atggagcgac acctgaagca gttccagctc aaggcgctct
     3721 gcgactataa gatcctgacc ctcaactcac agagacaggg ggatgtggaa acggctacaa
     3781 atgcccgctc tgcgggctct gagaaccaca ccccagctgc agagccacaa gcgtctcttt
     3841 cagaaaagac gccaaccaaa agaacagccg actctgttct caccgacgtt tcagaatcgc
     3901 cttcggattc agacactcct gtagaggata tcagttgcga agcgtgcaaa aatgcagaaa
     3961 aagatgaagg tccttattat acccatctag gagctggtcc taatgtggca gctattagaa
     4021 caatcatgga agaaaggttt ggagagaagg gtaaagctat tagaattgaa agagtcatat
     4081 atactggtaa agaaggcaag agctctcagg gatgtcctat tgctaaatgg gtgtatcgga
     4141 ggtcaagtac tgaggagaaa ctgctgtgtc tggtccgcgt ccgagccaag catacatgtg
     4201 acactgcagt gatcgtgatt gtcatcctgc tgtgggacgg aatcccgaag ccgctcgcgt
     4261 ccgagctcta ctcagaactc acagagattc tgagtaaccg tggcatatgc accaaccgcc
     4321 gctgtgctca gaatgaaaat agaaactgtt gttgtcaggg tgagaatcca gaaacctgtg
     4381 gtgcctcctt ttcttatggt tgttcttgga gcatgtacta taatggatgt aagtttgcca
     4441 gaagcaagaa cccaaggaaa tttaggctac atggagatga gccaaaagag gaagagaaac
     4501 ttggctctca cttgcaaaac ctggctactg ttattgctcc catatacaag aaacttgcac
     4561 ctgatgccta caggaatcag gtcgagtttg aacaccgagc tatagaatgc cgtttgggtc
     4621 tgaaggaagg ccggccattc tcaggggtca ctgcatgtct ggacttctct gctcatgccc
     4681 acagagacca gcagaacatg gccaatggca gtacagtggt ggtcaccctc actagagaag
     4741 acaatcgaga agtcggaggg cagccagagg atgagcagct ccatgtactg cctctgtaca
     4801 ccattgctac tgaggatgag tttgggagca cagaaggtca ggaggagaag attctgcagg
     4861 ggtccattca ggttctgcac tcttttcgga ggagaagagt caggaggtta gtggatccac
     4921 ccaagaattg ccgacagaag aaattggagg ccaagaaagc tagaaagctt tcttctctgg
     4981 agaattgctc gaataagaat gaaaaggaaa agtcttcctc acgtacaagg ccgatggaaa
     5041 atgcaagccc tatgatacag atgacagcac aacttcggct ttcgggatct gtcatccagc
     5101 agccacagtc actccagagg caccttcagc aagtgcagag gccacctcag ccacagccac
     5161 ctcagccaca gcacatcttg cccagtaact ctcaacctgt tggttctcat tcttctggat
     5221 ccaccaatgt atacatgaga cagcctgctc cacttagtcc ttaccccagc tcttcacacg
     5281 ctgcagatat gtacggagat gccaaccatg tgaacttcca ccccacttca tctcatgctg
     5341 cgggattata tttgagtcct tctaattcca tgaaccccta ccccgggctt ttcaatcaaa
     5401 ataaccaata cgcagcttct cagtgcaatg ggagcatgtc ggcagacagc ggtccccctt
     5461 tcttaggtcc ttattccccc cagtctcagt ccaggggtct gcatagatat ccaaaccagg
     5521 accatctcag caatctgaac ttaccaccca tccacacgct ttacccacag aggtcagaca
     5581 gcccctttaa gtacttaaat tatggaaacc aaaacaacgt gcagagagat agagatgcct
     5641 tcactaactg tcccttaaaa ccaaatgtac accacctacc aacgttttct tcttacgcca
     5701 cccccaagat ggatggccat tttatgggag ccgcctccag actaccgtac agccacccaa
     5761 acatggagta caaatccagt gaccatcatc tccctcccca catgattcac ggctacccca
     5821 cagcagcttc aggctcagtg agcagttcca gtcacgcctt ccacaacaag gagaatgaca
     5881 acatggtctc ccacacagcc aatgggttat caagagtgct tccagggttt aatcatgaca
     5941 gaactgcttc tgctcaaggt ccgttataca gtctgccgga ctacagtcag gaaaagcagc
     6001 ccggggtgtc aggccaggat ggggcttctg tggaggacat tgaggtttgg tcagacagtg
     6061 agcacaactt ccaggatcct tccatcggag gagtggcaat agcccccact catgggtcaa
     6121 ttctcattga gtgcgccaag tgtgaggtcc acgccacaac caaagtaaac aagcccaatc
     6181 ggaaaaagcc cgccaggatc tcacttgtct tttaccagca caagaacttg aatgagccaa
     6241 aacatagctt ggcggtctgg gaagccaaaa tggccggaaa ggcccggaaa gaagaggaag
     6301 agtgtgaaaa gtatggatca gaccacgtgt ctcagaaaaa tcacggcaaa cgggtaaagc
     6361 gtgagcccac ggggccacag gaaactccca agcccagtta cctgcgcttc atccattctc
     6421 ttgctgagaa cacagtgtct atgaccacag actcgaccgt gactacaagg ccatatgctt
     6481 tcactcaggt cacagggcct tacaacacat atgtaggatc cgactacaaa gaccatgacg
     6541 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag tgagggcccg
     6601 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6661 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6721 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6781 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6841 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6901 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6961 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7021 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7081 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7141 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7201 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     7261 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     7321 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     7381 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     7441 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     7501 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     7681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7861 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     7921 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8041 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8101 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8161 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8221 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8281 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     8341 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     8401 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8461 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8521 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8581 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8641 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8701 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8761 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8821 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8881 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8941 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     9001 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     9061 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     9121 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     9181 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     9241 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9301 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9421 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10081 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10321 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10561 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10621 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10681 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10741 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10801 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10861 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10921 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10981 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11041 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11101 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11161 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11221 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11281 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11341 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11401 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11461 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11521 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11581 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11641 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
    11701 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
    11761 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
    11821 aagaatctgc ttagg
//
