LOCUS       V042060                14487 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042060
VERSION     V042060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      750..759
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             780..1442
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             1542..9167
                     /label="Tln2(NM_001081242)"
                     /note="Tln2(NM_001081242)"
                     /gene="Tln2"
     CDS             9174..9239
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    9263..9851
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9917..9965
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10167..10595
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10609..10938
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             11005..11799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11975..12096
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(12145..12161)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12169..12185
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12193..12223)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12238..12259
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12547..13135)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13306..14166)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14167..14271)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga gagcgacgag agcggcctgc
      781 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg
      841 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca
      901 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc
      961 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg
     1021 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca
     1081 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc
     1141 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc
     1201 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg
     1261 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc
     1321 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca
     1381 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg
     1441 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca
     1501 ccggatctcg cggtggaggc ggatcaggag gcggtggatc tatggtggcc ctatccttaa
     1561 agatctgtgt ccgccactgc aatgtggtga agaccatgca gtttgagcca tctacagctg
     1621 tgtatgacgc ttgtcgagtc attagggaga gagtacctga ggcacagact gggcaagctt
     1681 ctgactatgg gctgtttctc tccgatgaag acccaaggaa agggatttgg ctagaagcag
     1741 gtcggacact ggattactac atgttgcgga atggggacat tctggagtac aagaagaaac
     1801 agaggcctca gaaaatccga atgttggatg gctctgtgaa gacggtgatg gtagatgact
     1861 ccaagacggt gggggagctc ctggtcacga tctgcagcag gataggaata acaaattatg
     1921 aagaatactc tttaatccaa gaaaccattg aagaaaagaa agaagaaggg acaggcacac
     1981 taaaaaaaga taggacgttg ttacgagatg agaggaagat ggagaagctg aaggccaaac
     2041 tgcacacaga tgatgactta aattggctag atcacagccg gacattcaga gagcaaggcg
     2101 tggatgaaaa tgaaacattg ttgctccgac ggaagttctt ttactctgat cagaatgtgg
     2161 actcgagaga cccggtgcag ttgaacttgc tttatgtcca ggctcgggat gacatcctga
     2221 atggctctca ccctgtctcc ttcgagaagg cctgtgagtt tggtgggttc caagcccaga
     2281 tacagtttgg acctcacgtg gaacacaaac acaagcctgg attcttagat ctaaaggagt
     2341 tcctgccaaa agagtacatc aagcagagag gagccgagaa gcgaatattt caggaacata
     2401 aaaactgtgg agagatgagt gaaatagaag ccaaggtgaa gtacgtcaaa ctcgcccggt
     2461 ccctccggac atatggggtg tcctttttcc tagtgaagga aaaaatgaaa ggcaagaaca
     2521 agttggttcc ccgtctcctg ggcatcacca aggactccgt catgcgtgtg gatgagaaga
     2581 ccaaggaggt gcttcaggag tggcctctca ccacggtcaa gcgctgggcc gcttcaccca
     2641 agagcttcac actggatttt ggggagtacc aagagagcta ctattcagta caaaccacag
     2701 agggggagca aatatcccag ctgattgcag gatacattga tatcatcctc aaaaagaaac
     2761 aaagcaaaga tagatttggc ctggaagggg atgaggagtc aactatgttg gaagaatcag
     2821 tttccccaaa aaaatctacc atcctgcagc agcagttcaa ccggactggg aaagcagagc
     2881 atggttcggt agcactgcca gctgtcatgc gctctggctc cagtgggcct gagacgttca
     2941 atgttggcag catgccgtca ccacagcagc aggtcatggt cggacagatg caccgaggac
     3001 acatgcctcc gctgacgtcg gcacagcaag cccttatggg gaccatcaac acaagcatgc
     3061 acgctgtcca gcaggctcag gatgacctca gcgagctcga ctcgctgcca cccctcggcc
     3121 aagatatggc atctagggta tgggttcaga acaaagtgga tgaatccaag catgaaatcc
     3181 actctcaagt cgatgctatc acagctggga cagcttcagt tgtcaacctc acagctggtg
     3241 accctgcaga cactgactac acagccgtgg gctgtgcaat cactaccatt tcttccaacc
     3301 tgaccgaaat gtccaagggg gtcaagctgc tggcagccct catggacgat gacgtgggca
     3361 gtggggagga tcttctcaga gctgccagga ccctcgccgg ggctgtgtct gatttgctga
     3421 aggctgtgca gcctacttca ggagagcctc gacagactgt tttgactgct gccggaagca
     3481 tcggccaagc cagtggagat ctcctcagac agattggaga gaacgagacg gatgagagat
     3541 tccaagatgt tttaatgagt ctagctaagg ctgttgccaa tgctgctgcc atgttagtac
     3601 tgaaggcgaa gaatgtggcc caggtggctg aagacaccgt cctacagaac agggtgatcg
     3661 ccgcagccac ccagtgtgcc ctctctacct cgcagcttgt ggcgtgtgct aaggtggtga
     3721 gccccaccat cagctctccc gtgtgccagg agcagctgat cgaagctggg aagctggtgg
     3781 accgctctgt ggaaaactgt gtccgagcct gccaggcggc caccagtgat agcgagctcc
     3841 tgaagcaggt cagcgcggcg gccagtgtgg tcagccaggc cctgcacgac ctcctgcagc
     3901 atgtgcggca gttcgccagc cgaggcgagc ccattggccg ctatgaccag gccaccgaca
     3961 ccatcatgtg tgtcactgag agcatcttca gttccatggg cgatgctggt gagatggtgc
     4021 gccaagcccg ggtgctagcc caggccacat cagatctcgt caatgccatg aggtcagatg
     4081 cggaggctga gattgacatg gagaattcca agaagctcct ggcggccgca aagctcttgg
     4141 ctgactccac tgcccgcatg gtggaagctg cgaagggggc tgcagccaac ccagagaatg
     4201 aagaccagca gcagaggctg agagaagctg ccgagggtct ccgagtagca accaatgccg
     4261 ctgcgcagaa tgctattaag aaaaaaatcg tcaaccggct ggaggttgca gccaagcagg
     4321 ctgcagctgc tgccacacag accatcgcag cctcccagaa tgcagccatc tccaacaaga
     4381 acccctcagc ccagcaacaa ctggtccaga gctgcaaggc ggtggctgat cacatccctc
     4441 agctagtgca gggcgtgaga gggagccaag cacaagcaga agacctcagt gcccagctgg
     4501 ctctcatcat ctccagccag aacttcctgc agcctggaag caagatggtg tcctctgcta
     4561 aggcggcggt gcccactgtg agtgaccagg ctgcagccat gcagttgagt cagtgtgcca
     4621 agaacctggc aaccagcctg gccgagctgc gcacagcctc gcagaaggcc cacgaagcct
     4681 gtggccccat ggaaatagat tctgcactga acactgtgca gactctcaag aatgagctgc
     4741 aggacgccaa gatggccgct gcagaaagtc aactgaaacc acttccagga gaaacgctgg
     4801 aaaagtgtgc tcaggacctg ggaagcacct ctaagggagt gggctcctcc atggcacagc
     4861 tcctgacctg tgctgctcaa ggcaatgagc attacacagg cgtggcagca agagaaacag
     4921 cccaggccct gaaaacactg gcccaggctg cccggggagt ggcggcatcc acaaatgatc
     4981 ctgaggctgc tcatgccatg ttagactctg ctcgggatgt gatggagggc tctgccatgc
     5041 tcatccagga agccaagcag gcactaattg cacctggaga cacagagagt cagcagagat
     5101 tagcccaggt ggccaaagct gtgtctcact cactgaataa ctgcgtgaat tgccttcctg
     5161 gacagaagga tgtggatgtc gccttgaaga gcatcgggga ggccagcaag aagctacttg
     5221 tagattcgct acctccaagc acaaagccct tccaggaggc ccagagtgag ctgaaccagg
     5281 ctgctgctga tctgaaccaa tctgctgggg aagtcgtcca tgccacaagg ggccagagtg
     5341 gagagctggc agccgcttct ggaaaattca gtgatgactt tgacgagttc ctggatgccg
     5401 gcattgagat ggctggccag gcacagacaa aagaagacca gatgcaagtg atagggaacc
     5461 tgaagaatat ctcgatggcg tccagcaagc tcctgctggc cgccaagtct ctctctgtag
     5521 atcctggagc tcccaacgca aaaaatctcc tggctgcagc tgcaagagct gtgacagaga
     5581 gcatcaatca gctcatcatg ctgtgtactc agcaggcccc tgggcagaag gagtgtgaca
     5641 acgccctgcg tgagctcgag actgtcaagg ggatgttgga aaacccgaat gagcctgtga
     5701 gcgacctctc ttattttgac tgcattgaga gtgtgatgga gaactccaag gttctgggcg
     5761 agtcaatggc agggatttca cagaatgcca agactggaga cctccctgcc ttcggagaat
     5821 gtgtggggat tgcatctaag gctctctgtg ggctgacaga ggcagcagcc caggctgcgt
     5881 atctggttgg catctctgat ccgaacagcc aggcaggtca ccaaggcctg gtggacccca
     5941 tccagtttgc cagggctaac caggcgatcc agatggcatg tcagaacttg gtggaccctg
     6001 gcagcagccc atcacaggtt ctgtcggctg ccactattgt tgccaagcac acatcagcct
     6061 tatgcaatgc ctgccgcatc gcctcatcca agacggccaa ccctgttgcc aagcggcact
     6121 ttgtccagtc agccaaggag gttgccaaca gcaccgccaa cctggtcaag accatcaagg
     6181 ccctggatgg ggatttctct gaagacaatc gcaataagtg tcgaattgct accacgccct
     6241 tgatcgaggc ggtggagaac ctaacagcgt ttgcatcaaa ccccgagttt gccagcattc
     6301 ctgcacagat cagctctgag ggctcccagg cgcaggaacc aatcttggtc tcagccaaga
     6361 ccatgctgga gagctcatcg tacctcatcc gcaccgcgcg ctctctggcc atcaacccca
     6421 aagatccgcc cacctggtcc gtgttggctg gacactccca cactgtgtct gactccatta
     6481 agagtctcat cacgtctatc agggacaaag ccccagggca gagggaatgt gactactcca
     6541 ttgatggcat caaccggtgc atcagggaca tcgagcaggc ctccctggcc gcggtcagcc
     6601 agagcctggc cacgagggat gacatctctg tggaggccct acaggagcag ctgacctcag
     6661 tggtccaaga aatcggacat cttattgatc ccattgccac agctgcccga ggagaagctg
     6721 cccaacttgg acataaggtg acacagctgg cgagctactt tgagcccttg atcttagctg
     6781 cagttggtgt cgcctccaag atgctggacc atcaacaaca gatgacagtg ctggaccaga
     6841 ccaagactct tgcagagtct gccctgcaga tgctgtatgc agccaaggaa ggtggaggta
     6901 accccaaggc acagcacacc cacgacgcca tcacagaggc tgcgcagctg atgaaggaag
     6961 ctgtggatga catcatggtg acactgaatg aagcagccag tgaggtggga ctggtgggag
     7021 gcatggtgga cgccatcgca gaggctatga gcaagctgga tgaaggcaca cctccagaac
     7081 caaagggaac gtttgttgac taccagacga ctgtggttaa atactccaaa gccattgccg
     7141 tcacagctca ggaaatgatg actaagtcgg ttactaaccc ggaggagttg ggaggcctgg
     7201 cttcacaaat gaccactgac tatgggcacc tggctctcca gggccagatg gcagcagcca
     7261 ccgccgaacc agaggagatt ggattccaga ttcgcacacg tgtgcaggac cttggccatg
     7321 gctgtatctt cctggtgcaa aaggcaggag ccctccaggt gtgccccaca gacagctaca
     7381 ccaagaggga gctgatcgag tgtgcccgct ctgtcactga gaaggtgtcc ttggtgctgt
     7441 ctgctctcca ggctggaaac aaggggaccc aggcatgcat cacggccgca actgcagttt
     7501 ctgggatcat tgccgatctg gacaccacca ttatgtttgc aacagctggc acactgaatg
     7561 cagagaacgg cgagaccttt gccgatcaca gggagaacat tctgaagaca gccaaggcct
     7621 tggtggaaga cacaaagctc ctcgtgtctg gggctgcatc cactccagac aagctggccc
     7681 aggccgctca gtcctcagca gccaccatca cccagctggc agaggtggtc aagctggggg
     7741 ccgccagcct gggctccaat gaccctgaga cccaggtggt actgataaat gccatcaaag
     7801 acgtggccaa ggccctttcc gatctcattg gtgctaccaa gggagctgcc agcaagccgg
     7861 ctgacgaccc ctccatgtac cagctcaaag gagccgccaa ggtgatggtg accaatgtca
     7921 cctcccttct taagactgtg aaggcagtgg aagacgaggc cacccggggc acaagggcac
     7981 ttgaggccac catcgagtac ataaaacagg agctcacagt gttccagtca aaagacatac
     8041 ctgaaaagac atcatcaccc gaggaatcaa taagaatgac gaaaggtatc accatggcaa
     8101 cagcgaaagc cgtggcagct gggaactcat gtagacaaga ggatgtaatt gcaaccgcca
     8161 atctgagcag gaaggctgtc tcagatatgt tgatagcttg caagcaagca tccttctacc
     8221 ctgatgtcag tgaagaggtg cgaaccagag ccttgcggta tggcacagaa tgcaccttgg
     8281 gctacctgga cctactagaa cacgtcttgg tgatccttca aaagccaacc ccggaactca
     8341 agcatcagct ggctgctttc tccaagagag ttgctggagc tgtgacggag ctcatccagg
     8401 cagcggaagc catgaaagga acagagtggg tggatccaga agacccaacc gtcattgcag
     8461 aaactgagtt actgggagct gcagcatcca ttgaagccgc tgccaagaag ttagagcagc
     8521 tgaagccaag agcaaagcca aagcaagcag acgagaccct ggattttgaa gaacagatct
     8581 tagaagctgc taaatccatt gctgctgcca caagtgccct ggtcaaatcg gcctcagcag
     8641 cccagaggga gctggtagcc caaggcaagg tgggctccat ccctgccaat gctgccgatg
     8701 atggacagtg gtcccagggg ctgatctctg ccgcccggat ggtggcggca gcaaccagca
     8761 gtctctgtga ggcagccaat gcctctgtgc agggacatgc cagtgaggaa aagctcatct
     8821 catccgccaa gcaggtcgca gcttcaaccg ctcagctgct ggtggcctgc aaggtgaagg
     8881 ccgaccagga ttcagaggcc atgaagcggc tacaggcggc aggaaatgct gtgaaaagag
     8941 cctcagacaa tcttgttcgt gcagcccaga aggcagcatt tggcaaagct gatgatgacg
     9001 atgttgtggt gaaaaccaag ttcgtgggag gcattgctca gatcattgca gcccaggagg
     9061 aaatgctgaa gaaagaacga gagctggagg aagccaggaa gaagctggcc cagatccgcc
     9121 agcagcagta caaattcctc cccacagagc tgagggaaga tgagggcgga tccgactaca
     9181 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     9241 agtgagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     9301 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     9361 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     9421 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9481 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9541 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     9661 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9721 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9781 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9841 cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     9901 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     9961 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    10021 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    10081 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
    10141 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    10201 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10261 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10321 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10381 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10441 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10501 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
    10561 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    10621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10861 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10921 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
    10981 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
    11041 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    11101 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    11161 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
    11221 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    11281 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    11341 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    11401 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    11461 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    11521 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    11581 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    11641 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    11701 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    11761 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
    11821 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    11881 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    11941 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    12061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    12121 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    12241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    12301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    12361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    12421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    12481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    12541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12721 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    13021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    13261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    13321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    13381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    13441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    13501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    13561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    13621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    13681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    14221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    14281 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact
    14341 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    14401 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    14461 gacaattgca tgaagaatct gcttagg
//
