LOCUS       V028284                 7596 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028284
VERSION     V028284
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3743
                     /label="Tlr3(NM_126166)"
                     /note="Tlr3(NM_126166)"
                     /gene="Tlr3"
     CDS             3762..3791
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3810..3833
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3853..3869)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3880..4502
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4651..5239)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5568..5615
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5847..6641)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6676..7033)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7035..7139)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7166..7594
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaagggtgt tcctcttatc taatgtactc ctttggggga cttttgtccc
     1081 tatggattct tctggtgtct tccacaaacc aatgcactgt gagatacaac gtagctgact
     1141 gcagccattt gaagctaaca cacatacctg atgatcttcc ctctaacata acagtgttga
     1201 atcttactca caaccaactc agaagattac cacctaccaa ctttacaaga tacagccaac
     1261 ttgctatctt ggatgcagga tttaactcca tttcaaaact ggagccagaa ctgtgccaaa
     1321 tactcccttt gttgaaagta ttgaacctgc aacataatga gctctctcag atttctgatc
     1381 aaacctttgt cttctgcacg aacctgacag aactcgatct aatgtctaac tcaatacaca
     1441 aaattaaaag caaccctttc aaaaaccaga agaatctaat caaattagat ttgtctcata
     1501 atggtttatc atctacaaag ttgggaacgg gggtccaact ggagaacctc caagaactgc
     1561 tcttagcaaa aaataaaatc cttgcgttgc gaagtgaaga acttgagttt cttggcaatt
     1621 cttctttacg aaagttggac ttgtcatcaa atccacttaa agagttctcc ccggggtgtt
     1681 tccagacaat tggcaagtta ttcgccctcc tcttgaacaa cgcccaactg aacccccacc
     1741 tcacagagaa gctttgctgg gaactttcaa acacaagcat ccagaatctc tctctggcta
     1801 acaaccagct gctggccacc agcgagagca ctttctctgg gctgaagtgg acaaatctca
     1861 cccagctcga tctttcctac aacaacctcc atgatgtcgg caacggttcc ttctcctatc
     1921 tcccaagcct gaggtatctg tctctggagt acaacaatat acagcgtctg tcccctcgct
     1981 ctttttatgg actctccaac ctgaggtacc tgagtttgaa gcgagcattt actaagcaaa
     2041 gtgtttcact tgcttcacat cccaacattg acgatttttc ctttcaatgg ttaaaatatt
     2101 tggaatatct caacatggat gacaataata ttccaagtac caaaagcaat accttcacgg
     2161 gattggtgag tctgaagtac ctaagtcttt ccaaaacttt cacaagtttg caaactttaa
     2221 caaatgaaac atttgtgtca cttgctcatt ctcccttgct cactctcaac ttaacgaaaa
     2281 atcacatctc aaaaatagca aatggtactt tctcttggtt aggccaactc aggatacttg
     2341 atctcggcct taatgaaatt gaacaaaaac tcagcggcca ggaatggaga ggtctgagaa
     2401 atatatttga gatctaccta tcctataaca aatacctcca actgtctacc agttcctttg
     2461 cattggtccc cagccttcaa agactgatgc tcaggagggt ggcccttaaa aatgtggata
     2521 tctccccttc acctttccgc cctcttcgta acttgaccat tctggactta agcaacaaca
     2581 acatagccaa cataaatgag gacttgctgg agggtcttga gaatctagaa atcctggatt
     2641 ttcagcacaa taacttagcc aggctctgga aacgcgcaaa ccccggtggt cccgttaatt
     2701 tcctgaaggg gctgtctcac ctccacatct tgaatttaga gtccaacggc ttagatgaaa
     2761 tcccagtcgg ggttttcaag aacttattcg aactaaagag catcaatcta ggactgaata
     2821 acttaaacaa acttgaacca ttcatttttg atgaccagac atctctaagg tcactgaacc
     2881 tccagaagaa cctcataaca tctgttgaga aggatgtttt cgggccgcct tttcaaaacc
     2941 tgaacagttt agatatgcgc ttcaatccgt tcgactgcac gtgtgaaagt atttcctggt
     3001 ttgttaactg gatcaaccag acccacacta atatctctga gctgtccact cactacctct
     3061 gtaacactcc acatcattat tatggcttcc ccctgaagct tttcgataca tcatcctgta
     3121 aagacagcgc cccctttgaa ctcctcttca taatcagcac cagtatgctc ctggttttta
     3181 tacttgtggt actgctcatt cacatcgagg gctggaggat ctctttttac tggaatgttt
     3241 cagtgcatcg gattcttggt ttcaaggaaa tagacacaca ggctgagcag tttgaatata
     3301 cagcctacat aattcatgcc cataaagaca gagactgggt ctgggaacat ttctccccaa
     3361 tggaagaaca agaccaatct ctcaaatttt gcctagaaga aagggacttt gaagcaggcg
     3421 tccttggact tgaagcaatt gttaatagca tcaaaagaag ccgaaaaatc attttcgtta
     3481 tcacacacca tttattaaaa gaccctctgt gcagaagatt caaggtacat cacgcagttc
     3541 agcaagctat tgagcaaaat ctggattcaa ttatactgat ttttctccag aatattccag
     3601 attataaact aaaccatgca ctctgtttgc gaagaggaat gtttaaatct cattgcatct
     3661 tgaactggcc agttcagaaa gaacggataa atgcctttca tcataaattg caagtagcac
     3721 ttggatctcg gaattcagca catacgcgta cgcggccgct cgagcagaaa ctcatctcag
     3781 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     3841 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     3901 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3961 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     4021 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     4081 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     4141 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4201 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4261 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4321 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     4381 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     4441 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     4501 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4561 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4621 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4681 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4741 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4801 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4861 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4921 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4981 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5041 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5101 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5161 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5221 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5281 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5341 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     5401 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     5461 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     5521 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     5581 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     5641 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     5701 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     5761 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     5821 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     5881 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     5941 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     6001 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     6061 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     6121 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     6181 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     6241 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     6301 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     6361 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     6421 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     6481 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     6541 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     6601 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     6661 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6721 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6781 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6841 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6901 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6961 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     7021 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7141 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7201 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7261 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7321 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7381 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7441 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7501 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7561 aatgagctga tttaacaaaa atttaacgcg aatttt
//
