LOCUS       V041342                 5620 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041342
VERSION     V041342
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1775
                     /label="Tnfrsf13b(NM_021349)"
                     /note="Tnfrsf13b(NM_021349)"
                     /gene="Tnfrsf13b"
     CDS             1782..1808
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1812..1838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1842..1868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     primer_bind     complement(1877..1893)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1904..2526
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2675..3263)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3592..3639
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3871..4665)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4700..5057)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5059..5163)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5190..5618
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggctatggca ttctgcccca aagatcagta ctgggactcc tcaaggaaat
     1081 cctgtgtctc ctgtgcactg acctgcagcc agaggagcca gcgcacctgt acagacttct
     1141 gcaaattcat caattgccga aaagagcaag gcaggtacta cgaccatctc ctgggggcct
     1201 gcgtcagctg tgactccacc tgcacacagc accctcagca gtgtgcccac ttctgtgaga
     1261 aaaggcccag aagccaggcg aacctccagc ccgagctcgg gagaccacag gccggggagg
     1321 tggaagtcag gtcagacaac tcaggaaggc accagggatc tgagcatggt ccaggattga
     1381 ggctaagtag cgaccagctg actctctact gcacactggg ggtctgcctc tgcgccatct
     1441 tctgctgttt cttggtggcc ttggcctcct tcctcaggcg tagaggagag ccactaccca
     1501 gccagcctgc cgggccacgt gggtcacaag caaactctcc ccacgcccac cgccccgtga
     1561 cagaggcttg cgacgaggtg accgcgtcac cccagcctgt ggaaacgtgt agcttctgct
     1621 tcccggagcg cagttctccc actcaggaga gcgcgccgcg ttcgctcggg atacacggct
     1681 tcgcgggcac tgccgccccg cagccctgta tgcgtgcaac agtaggcggc ctgggtgtcc
     1741 tgcgcgcatc cactggggac gctcgtccgg caactacgcg ttacccatac gacgtacctg
     1801 attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat gatgtcccag
     1861 actacgcata gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga
     1921 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     1981 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     2041 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     2101 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     2161 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     2221 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     2281 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     2341 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata
     2401 ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca
     2461 tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt
     2521 tgaattgggt acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2581 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     3421 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     3481 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     3541 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     3601 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     3661 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     3721 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     3781 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     3841 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3901 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3961 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc
     4021 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     4081 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     4141 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     4201 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     4261 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     4321 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     4381 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     4441 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc
     4501 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     4561 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     4621 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     4681 tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     5041 ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5161 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     5221 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5281 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5341 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5401 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5461 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5521 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5581 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
