LOCUS       V029958                 5756 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029958
VERSION     V029958
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..1918
                     /label="Tra2b(NM_009186)"
                     /note="Tra2b(NM_009186)"
                     /gene="Tra2b"
     CDS             1922..1951
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1970..1993
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2013..2029)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2040..2662
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2811..3399)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3728..3775
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4007..4801)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4836..5193)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5195..5299)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5326..5754
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatgagc gacagcggcg agcagaacta
     1081 cggcgagcgg gaatcccgtt ctgcttcccg aagtggaagt gctcacggat cggggaaatc
     1141 tgcacggcat acacctgcaa ggtctcgctc caaggaagac tcaaggcgtt ctagatcaaa
     1201 gtccaggtcc aggtctgaat ctaggtctag atccagaaga agttctagaa ggcattatac
     1261 aaggtcacga tcacgatctc gctcgcatag acgatcccgg agcaggtctt acagccgaga
     1321 ttatcgcagg cgccacagcc acagccattc tcccatgtct actcgaaggc gtcatgttgg
     1381 gaaccgggca aatcctgacc ccaactgttg tcttggcgtg tttgggttga gcttatacac
     1441 cacagaaaga gacctaagag aagtgttctc taaatatggc cccattgctg atgtgtctat
     1501 tgtatatgac cagcaatcta gacgttcaag aggatttgcc tttgtatatt ttgaaaatgt
     1561 agacgatgcc aaggaagcta aagaacgtgc caatggaatg gagcttgatg ggcgtcgaat
     1621 tagagtcgat ttctctataa caaaaaggcc ccatacccca acaccaggaa tttatatggg
     1681 gagacccact tatggcagtt ctcgccgccg agactattat gacagagggt acgatcgggg
     1741 ttatgatgac cgggactatt acagcagatc atacagagga ggaggtggtg gaggaggtgg
     1801 atggagagca gctcaagaca gggatcagat ttacagaaga cggtcacctt ctccttacta
     1861 cagtcgtgga ggatacaggt cacgttctcg atcacgatcc tactcacctc gtcgctacct
     1921 cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg attacaagga
     1981 tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga acagatcccg
     2041 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     2101 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     2161 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     2221 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2281 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2341 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2401 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     2461 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     2521 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
     2581 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
     2641 ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc agctcactca
     2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3541 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
     3601 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
     3661 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
     3721 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
     3781 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
     3841 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     3901 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     3961 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     4021 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     4081 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     4141 tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     4201 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     4261 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     4321 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     4381 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     4441 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     4501 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     4561 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     4621 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     4681 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     4741 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     4801 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
     4861 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     4921 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     4981 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     5041 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     5101 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     5161 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa tattattgaa
     5221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5641 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aatttt
//
