LOCUS V029958 5756 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029958 VERSION V029958 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1055..1918 /label="Tra2b(NM_009186)" /note="Tra2b(NM_009186)" /gene="Tra2b" CDS 1922..1951 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1970..1993 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2013..2029) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2040..2662 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2811..3399) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3728..3775 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4007..4801) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4836..5193) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5195..5299) /gene="bla" /label="AmpR promoter" rep_origin 5326..5754 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgcggc gcgccagatc tcaagcttgc caccatgagc gacagcggcg agcagaacta 1081 cggcgagcgg gaatcccgtt ctgcttcccg aagtggaagt gctcacggat cggggaaatc 1141 tgcacggcat acacctgcaa ggtctcgctc caaggaagac tcaaggcgtt ctagatcaaa 1201 gtccaggtcc aggtctgaat ctaggtctag atccagaaga agttctagaa ggcattatac 1261 aaggtcacga tcacgatctc gctcgcatag acgatcccgg agcaggtctt acagccgaga 1321 ttatcgcagg cgccacagcc acagccattc tcccatgtct actcgaaggc gtcatgttgg 1381 gaaccgggca aatcctgacc ccaactgttg tcttggcgtg tttgggttga gcttatacac 1441 cacagaaaga gacctaagag aagtgttctc taaatatggc cccattgctg atgtgtctat 1501 tgtatatgac cagcaatcta gacgttcaag aggatttgcc tttgtatatt ttgaaaatgt 1561 agacgatgcc aaggaagcta aagaacgtgc caatggaatg gagcttgatg ggcgtcgaat 1621 tagagtcgat ttctctataa caaaaaggcc ccatacccca acaccaggaa tttatatggg 1681 gagacccact tatggcagtt ctcgccgccg agactattat gacagagggt acgatcgggg 1741 ttatgatgac cgggactatt acagcagatc atacagagga ggaggtggtg gaggaggtgg 1801 atggagagca gctcaagaca gggatcagat ttacagaaga cggtcacctt ctccttacta 1861 cagtcgtgga ggatacaggt cacgttctcg atcacgatcc tactcacctc gtcgctacct 1921 cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg attacaagga 1981 tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga acagatcccg 2041 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 2101 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 2161 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 2221 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 2281 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 2341 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 2401 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 2461 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 2521 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg 2581 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca 2641 ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc agctcactca 2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 3241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 3541 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc 3601 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat 3661 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg 3721 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata 3781 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg 3841 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa 3901 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc 3961 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt 4021 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga 4081 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta 4141 tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc 4201 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc 4261 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct 4321 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga 4381 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc 4441 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat 4501 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga 4561 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt 4621 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg 4681 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc 4741 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca 4801 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa 4861 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa 4921 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc 4981 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc 5041 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga 5101 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga 5161 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa tattattgaa 5221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 5281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg 5341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 5401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 5461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 5521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 5581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 5641 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 5701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aatttt //