LOCUS       V026843                 6051 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026843
VERSION     V026843
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2198
                     /label="Trp53(NM_011640)"
                     /note="Trp53(NM_011640)"
                     /gene="Trp53"
     CDS             2217..2246
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2265..2288
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2308..2324)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2335..2957
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3106..3694)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4023..4070
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4302..5096)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5131..5488)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5490..5594)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5621..6049
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gactgccatg gaggagtcac agtcggatat cagcctcgag ctccctctga
     1081 gccaggagac attttcaggc ttatggaaac tacttcctcc agaagatatc ctgccatcac
     1141 ctcactgcat ggacgatctg ttgctgcccc aggatgttga ggagtttttt gaaggcccaa
     1201 gtgaagccct ccgagtgtca ggagctcctg cagcacagga ccctgtcacc gagacccctg
     1261 ggccagtggc ccctgcccca gccactccat ggcccctgtc atcttttgtc ccttctcaaa
     1321 aaacttacca gggcaactat ggcttccacc tgggcttcct gcagtctggg acagccaagt
     1381 ctgttatgtg cacgtactct cctcccctca ataagctatt ctgccagctg gcgaagacgt
     1441 gccctgtgca gttgtgggtc agcgccacac ctccagctgg gagccgtgtc cgcgccatgg
     1501 ccatctacaa gaagtcacag cacatgacgg aggtcgtgag acgctgcccc caccatgagc
     1561 gctgctccga tggtgatggc ctggctcctc cccagcatct tatccgggtg gaaggaaatt
     1621 tgtatcccga gtatctggaa gacaggcaga cttttcgcca cagcgtggtg gtaccttatg
     1681 agccacccga ggccggctct gagtatacca ccatccacta caagtacatg tgtaatagct
     1741 cctgcatggg gggcatgaac cgccgaccta tccttaccat catcacactg gaagactcca
     1801 gtgggaacct tctgggacgg gacagctttg aggttcgtgt ttgtgcctgc cctgggagag
     1861 accgccgtac agaagaagaa aatttccgca aaaaggaagt cctttgccct gaactgcccc
     1921 cagggagcgc aaagagagcg ctgcccacct gcacaagcgc ctctcccccg caaaagaaaa
     1981 aaccacttga tggagagtat ttcaccctca agatccgcgg gcgtaaacgc ttcgagatgt
     2041 tccgggagct gaatgaggcc ttagagttaa aggatgccca tgctacagag gagtctggag
     2101 acagcagggc tcactccagc tacctgaaga ccaagaaggg ccagtctact tcccgccata
     2161 aaaaaacaat ggtcaagaaa gtggggcctg actcagacac gcgtacgcgg ccgctcgagc
     2221 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     2281 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     2341 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     2401 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     2461 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     2521 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     2581 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     2641 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     2701 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     2761 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     2821 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     2881 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     2941 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     3001 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3541 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3841 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     3901 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     3961 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     4021 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     4081 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     4141 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     4201 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     4261 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     4321 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     4381 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     4441 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     4501 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     4561 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4621 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4681 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4741 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4801 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4861 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     4921 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4981 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5041 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     5101 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     5161 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5221 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5281 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     5341 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     5401 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     5461 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     5521 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5581 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     5641 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5701 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5761 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5821 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5881 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5941 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6001 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
