LOCUS       V018442                 5894 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018442
VERSION     V018442
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..2065
                     /label="UBE2Z(NM_023079)"
                     /note="UBE2Z(NM_023079)"
                     /gene="UBE2Z"
     CDS             2072..2089
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2108..2131
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2151..2167)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2178..2800
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2949..3537)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3866..3913
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4145..4939)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4974..5331)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5333..5437)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5464..5892
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatggcgg agagtccgac
     1021 tgaggaggcg gcaacggcgg gcgccggggc ggcgggcccc ggggcgagca gcgttgctgg
     1081 tgttgttggc gttagcggca gcggcggcgg gttcgggccg cctttcctgc cggatgtgtg
     1141 ggcggcggcg gcggcagcgg gcggggccgg gggcccgggg agcggcctgg ctccgctgcc
     1201 cgggctcccg ccctcagccg ctgcccacgg ggccgcgctg cttagccact gggaccccac
     1261 gctcagctcc gactgggacg gcgagcgcac cgcgccgcag tgtctactcc ggatcaagcg
     1321 ggatatcatg tccatttata aggagcctcc tccaggaatg ttcgttgtac ctgatactgt
     1381 tgacatgact aagattcatg cattgatcac aggcccattt gacactcctt atgaaggggg
     1441 tttcttcctg ttcgtgtttc ggtgtccgcc cgactatccc atccacccac ctcgggtcaa
     1501 actgatgaca acgggcaata acacagtgag gtttaacccc aacttctacc gcaatgggaa
     1561 agtctgcttg agtattctag gtacatggac tggacctgcc tggagcccag cccagagcat
     1621 ctcctcagtg ctcatctcta tccagtccct gatgactgag aacccctatc acaatgagcc
     1681 cggctttgaa caggagagac atccaggaga cagcaaaaac tataatgaat gtatccggca
     1741 cgagaccatc agagttgcag tctgtgacat gatggaagga aagtgtccct gtcctgaacc
     1801 cctacgaggg gtgatggaga agtcctttct ggagtattac gacttctacg aggtggcctg
     1861 caaagatcgc ctgcaccttc aaggccaaac tatgcaggac ccttttggag agaagcgggg
     1921 ccactttgac taccagtccc tcttgatgcg cctgggactg atacgtcaga aagtgctgga
     1981 gaggctccat aatgagaatg cagaaatgga ctctgatagc agttcatctg ggacagagac
     2041 agaccttcat gggagcctga gggttctcga gcatcatcac catcaccatg cagcaaatga
     2101 tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg gtcatagctg
     2161 tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     2221 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     2281 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     2341 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     2401 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     2461 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     2521 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     2581 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     2641 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
     2701 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
     2761 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga
     2821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3121 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3361 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3661 agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg gcctgccgcc
     3721 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag
     3781 gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag
     3841 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt tttattctgt
     3901 ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt
     3961 agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     4021 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     4081 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     4141 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     4201 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     4261 cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg ccacagtcga
     4321 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     4381 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     4441 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     4501 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     4561 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     4621 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     4681 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     4741 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     4801 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     4861 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     4921 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa
     4981 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     5041 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     5101 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     5161 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     5221 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     5281 tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca ggactcttcc
     5341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5461 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5521 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5581 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5641 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5701 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5761 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5821 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5881 ttaacgcgaa tttt
//
