LOCUS       V029962                14443 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029962
VERSION     V029962
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..9102
                     /label="UBR5(NM_015902)"
                     /note="UBR5(NM_015902)"
                     /gene="UBR5"
     CDS             9109..9135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9139..9165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9169..9195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    9219..9807
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9873..9921
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10123..10551
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10565..10894
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10961..11755
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11931..12052
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(12101..12117)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12125..12141
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12149..12179)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12194..12215
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12503..13091)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13262..14122)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14123..14227)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgac gtccatccat
      721 ttcgtggttc acccgctgcc gggcaccgag gaccagctca atgacaggtt acgagaagtt
      781 tctgagaagc tgaacaaata taatttaaac agccaccccc ctttgaatgt attggaacag
      841 gctactatta aacagtgtgt ggtgggacca aatcatgctg cctttcttct tgaggatggt
      901 agagtttgca ggattggttt ttcagtacag ccagacagat tggaattggg taaacctgat
      961 aataatgatg ggtcaaagtt gaacagcaac tcgggggcag ggaggacgtc aaggcctggt
     1021 aggacaagcg actctccatg gtttctctca ggttctgaga ctctaggcag gctggcaggc
     1081 aacaccttag gaagccgctg gagttctgga gtgggtggaa gtggtggagg atcctctggt
     1141 aggtcatcag ctggagctcg agattcccgc cggcagactc gagttattcg gacaggacgg
     1201 gatcgagggt ctgggctttt gggcagtcag ccccagccag ttattccagc atctgtcatt
     1261 ccagaggagc tgatttcaca ggcccaagtt gttttacaag gcaaatccag aagtgtcatt
     1321 attcgagaac ttcagagaac aaatcttgat gtgaaccttg ctgtaaataa tttacttagc
     1381 cgggatgatg aagatggaga tgatggggat gatacagcca gcgaatctta tttgcctgga
     1441 gaggatctta tgtctctcct tgatgccgac attcattctg cccacccaag tgtcattatt
     1501 gatgcagatg ccatgttttc tgaagacatt agctattttg gttacccttc ttttcgtcgt
     1561 tcatcacttt ccaggctagg ctcatctcga gttctccttc ttcccttaga gagagactct
     1621 gagctgttgc gtgaacgtga atccgtttta cgtttacgtg aacgaaggtg gcttgatgga
     1681 gcctcatttg ataatgaaag gggttctacc agcaaggaag gagagccaaa cttggataag
     1741 aagaatacac ctgttcaaag tccagtatct ctaggagaag atttgcagtg gtggcctgat
     1801 aaggatggaa caaaattcat ctgtattggg gctctgtatt ctgaacttct ggctgtcagc
     1861 agtaaaggag aactttatca gtggaaatgg agtgaatctg agccttacag aaatgcccag
     1921 aatccttcat tacatcatcc acgagcaaca tttttggggt taaccaatga aaagatagtc
     1981 ctcctgtctg caaatagcat aagagcaact gtagctacag aaaataacaa ggttgctaca
     2041 tgggtggatg aaactttaag ttctgtggct tctaaattag agcacactgc tcagacttac
     2101 tctgaacttc aaggagagcg gatagtttct ttacattgct gtgcccttta cacctgcgct
     2161 cagctggaaa acagtttata ttggtggggt gtagttcctt ttagtcaaag gaagaaaatg
     2221 ttagagaaag ctagagcaaa aaataaaaag cctaaatcca gtgctggtat ttcttcaatg
     2281 ccgaacatca ctgttggtac ccaggtatgc ttgagaaata atcctcttta tcatgctgga
     2341 gcagttgcat tttcaattag tgctgggatt cctaaagttg gtgtcttaat ggagtcagtt
     2401 tggaatatga atgacagctg tagatttcaa cttagatctc ctgaaagctt gaaaaacatg
     2461 gaaaaagcta gcaaaactac tgaagctaag cctgaaagta agcaggagcc agtgaaaaca
     2521 gaaatgggtc ctccaccatc tccagcatcc acgtgtagtg atgcatcctc aattgccagc
     2581 agtgcatcaa tgccatacaa acgacgacgg tcaacccctg caccaaaaga agaggaaaag
     2641 gtgaatgaag agcagtggtc tcttcgggaa gtggtttttg tggaagatgt caagaatgtt
     2701 cctgttggca aggtgctaaa agtagatggt gcctatgttg ctgtaaaatt tccaggaacc
     2761 tccagtaata ctaactgtca gaacagctct ggtccagatg ctgacccttc ttctctcctg
     2821 caggattgta ggttacttag aattgatgaa ttgcaggttg tcaaaactgg tggaacaccg
     2881 aaggttcccg actgtttcca aaggactcct aaaaagcttt gtatacctga aaaaacagaa
     2941 atattagcag tgaatgtaga ttccaaaggt gttcatgctg ttctgaagac tggaaattgg
     3001 gtacgatact gtatctttga tcttgctaca ggaaaagcag aacaggaaaa taattttcct
     3061 acaagcagca ttgctttcct tggtcagaat gagaggaatg tagccatttt cactgctgga
     3121 caggaatctc ccattattct tcgagatgga aatggtacca tctacccaat ggccaaagat
     3181 tgcatgggag gaataaggga tcccgattgg ctggatcttc cacctattag tagtcttgga
     3241 atgggtgtgc attctttaat aaatcttcct gccaattcaa caatcaaaaa gaaagctgct
     3301 gttatcatca tggctgtaga gaaacaaacc ttaatgcaac acattctgcg ctgtgactat
     3361 gaggcctgtc gacaatatct aatgaatctt gagcaagcgg ttgttttaga gcagaatcta
     3421 cagatgctgc agacattcat cagccacaga tgtgatggaa atcgaaatat tttgcatgct
     3481 tgtgtatcag tttgctttcc aaccagcaat aaagaaacta aagaagaaga ggaagcggag
     3541 cgttctgaaa gaaatacatt tgcagaaagg ctttctgctg ttgaggccat tgcaaatgca
     3601 atatcagttg tttcaagtaa tggcccaggt aatcgggctg gatcatcaag tagccgaagt
     3661 ttgagattac gggaaatgat gagacgttcg ttgagagcag ctggtttggg tagacatgaa
     3721 gctggagctt catccagtga ccaccaggat ccagtttcac cccccatagc tccccctagt
     3781 tgggttcctg accctcctgc gatggatcct gatggtgaca ttgattttat cctggccccc
     3841 gctgtgggat ctcttaccac agcagcaacc ggtactggtc aaggaccaag cacctccact
     3901 attccaggtc cttccacaga gccatctgta gtagaatcca aggatcgaaa ggcgaatgct
     3961 cattttatat tgaaattgtt atgtgacagt gtggttctcc agccctatct acgagaactt
     4021 ctttctgcca aggatgcaag agggatgacc ccatttatgt cagctgtaag tggccgagct
     4081 tatcctgctg caattaccat cttagaaact gctcagaaaa ttgcaaaagc tgaaatatcc
     4141 tcaagtgaaa aagaggaaga tgtattcatg ggaatggttt gcccatcagg taccaaccct
     4201 gatgactctc ctttatatgt tttatgttgt aatgacactt gcagttttac atggactgga
     4261 gcagagcaca ttaaccagga tatttttgag tgtcgaactt gtggcttgct ggagtcactg
     4321 tgttgttgta cggaatgtgc aagggtttgt cataaaggtc atgattgcaa actcaaacgg
     4381 acatcaccaa cagcctactg tgattgttgg gagaaatgta aatgtaaaac tcttattgct
     4441 ggacagaaat ctgctcgtct tgatctactt tatcgcctgc tcactgctac taatctggtt
     4501 actctgccaa acagcagggg agagcacctc ttactattct tagtacagac agtcgcaagg
     4561 cagacggtgg agcattgtca atacaggcca cctcgaatca gggaagatcg taaccgaaaa
     4621 acagccagtc ctgaagattc agatatgcca gatcatgatt tagagcctcc aagatttgcc
     4681 cagcttgcat tggagcgtgt tctacaggac tggaatgcct tgaaatctat gattatgttt
     4741 gggtcgcagg agaataaaga ccctcttagt gccagcagta gaataggcca tcttttgcca
     4801 gaagagcaag tatacctcaa tcagcaaagt ggcacaattc ggctggactg tttcactcat
     4861 tgccttatag ttaagtgtac agcagatatt ttgcttttag atactctact aggtacacta
     4921 gtgaaagaac tccaaaacaa atatacacct ggacgtagag aagaagctat tgctgtgaca
     4981 atgaggtttc tacgttcagt ggcaagagtt tttgttattc tgagtgtgga aatggcttca
     5041 tccaaaaaga aaaacaactt tattccacag ccaattggaa aatgcaagcg tgtattccaa
     5101 gcattgctac cttacgctgt ggaagaattg tgcaacgtag cagagtcact gattgttcct
     5161 gtcagaatgg ggattgctcg tccaactgca ccatttaccc tggctagtac tagcatagat
     5221 gccatgcagg gcagtgaaga attattttca gtggaaccac taccaccacg accatcatct
     5281 gatcagtcta gcagctccag tcagtctcag tcatcctaca tcatcaggaa tccacagcag
     5341 aggcgcatca gccagtcaca gcccgttcgg ggcagagatg aagaacagga tgatattgtt
     5401 tcagcagatg tggaagaggt tgaggtggtg gagggtgtgg ctggagaaga ggatcatcat
     5461 gatgaacagg aagaacacgg ggaagaaaat gctgaggcag agggacaaca tgatgagcat
     5521 gatgaagacg ggagtgatat ggagctggac ttgttagcag cagctgaaac agaaagtgat
     5581 agtgaaagta accacagcaa ccaagataat gctagtgggc gcagaagcgt tgtcactgca
     5641 gcaactgctg gttcagaagc aggagcaagc agtgttcctg ccttcttttc tgaagatgat
     5701 tctcaatcga atgactcaag tgattctgat agcagtagta gtcagagtga cgacatagaa
     5761 caggagacct ttatgcttga tgagccatta gaaagaacca caaatagctc ccatgccaat
     5821 ggtgctgccc aagctccccg ttcaatgcag tgggctgtcc gcaacaccca gcatcagcga
     5881 gcagccagta cagccccttc cagtacatct acaccagcag caagttcagc gggtttgatt
     5941 tatattgatc cttcaaactt acgccggagt ggtaccatca gtacaagtgc tgcagctgca
     6001 gcagctgctt tggaagctag caacgccagc agttacctaa catctgcaag cagtttagcc
     6061 agggcttaca gcattgtcat tagacaaatc tcggacttga tgggccttat tcctaagtat
     6121 aatcacctag tatactctca gattccagca gctgtgaaat tgacttacca agatgcagta
     6181 aacttacaga actatgtaga agaaaagctt attcccactt ggaactggat ggtcagtatt
     6241 atggattcta ctgaagctca attacgttat ggttctgcat tagcatctgc tggtgatcct
     6301 ggacatccaa atcatcctct tcacgcttct cagaattcag cgagaagaga gaggatgact
     6361 gcgcgagaag aagctagctt acgaacactt gaaggcagac gacgtgccac cttgcttagc
     6421 gcccgtcaag gaatgatgtc tgcacgagga gacttcctaa attatgctct gtctctaatg
     6481 cggtctcata atgatgagca ttctgatgtt cttccagttt tggatgtttg ctcattgaag
     6541 catgtggcat atgtttttca agcacttata tactggatta aggcaatgaa tcagcagaca
     6601 acattggata cacctcaact agaacgcaaa aggacgcgag aactcttgga actgggtatt
     6661 gataatgaag attcagaaca tgaaaatgat gatgacacca atcaaagtgc tactttgaat
     6721 gataaggatg atgactctct tcctgcagaa actggccaaa accatccatt tttccgacgt
     6781 tcagactcca tgacattcct tgggtgtata cccccaaatc catttgaagt gcctctggct
     6841 gaagccatcc ccttggctga tcagccacat ctgttgcagc caaatgctag aaaggaggat
     6901 ctttttggcc gtccaagtca gggtctttat tcttcatctg ccagtagtgg gaaatgttta
     6961 atggaggtta cagtggatag aaactgccta gaggttcttc caacaaaaat gtcttatgct
     7021 gccaatctga aaaatgtaat gaacatgcaa aaccggcaaa aaaaagaagg ggaagaacag
     7081 cccgtgctgc cagaagaaac tgagagttca aaaccagggc catctgctca tgatcttgct
     7141 gcacaattaa aaagtagctt actagcagaa ataggactta ctgaaagtga agggccacct
     7201 ctcacatctt tcaggccaca gtgtagcttt atgggaatgg ttatttccca tgatatgctg
     7261 ctaggacgtt ggcgcctttc tttagaactg ttcggcaggg tattcatgga agatgttgga
     7321 gcagaacctg gatcaatcct aactgaattg ggtggttttg aggtaaaaga atcaaaattc
     7381 cgcagagaaa tggaaaaact gagaaaccag cagtcaagag atttgtcact agaggttgat
     7441 cgggatcgag atcttctcat tcagcagact atgaggcagc ttaacaatca ctttggtcga
     7501 agatgtgcta ctacaccaat ggctgtacac agagtaaaag tcacatttaa ggatgagcca
     7561 ggagagggca gtggtgtagc acgaagtttt tatacagcca ttgcacaagc atttttatca
     7621 aatgaaaaat tgccaaatct agagtgtatc caaaatgcca acaaaggcac ccacacaagt
     7681 ttaatgcaga gattaaggaa ccgaggagag agagaccggg aaagggagag agaaagggaa
     7741 atgaggagga gtagtggttt gcgagcaggt tctcggaggg accgggatag agactttaga
     7801 agacagcttt ccatcgacac taggcccttt agaccagcct ctgaagggaa tcctagcgat
     7861 gatcctgagc ctttgccagc acatcggcag gcacttggag agaggcttta tcctcgtgta
     7921 caagcaatgc aaccagcatt tgcaagtaaa atcactggca tgttgttgga attatcccca
     7981 gctcagctgc ttctccttct agcaagtgag gattctctga gagcaagagt ggatgaggcc
     8041 atggaactca ttattgcaca tggacgggaa aatggagctg atagtatcct ggatcttgga
     8101 ttagtagact cctcagaaaa ggtacagcag gaaaaccgaa agcgccatgg ctctagtcga
     8161 agtgtagtag atatggattt agatgataca gatgatggtg atgacaatgc ccctttgttt
     8221 taccaacctg ggaaaagagg attttatact ccaaggcctg gcaagaacac agaagcaagg
     8281 ttgaattgtt tcagaaacat tggcaggatt cttggactat gtctgttaca gaatgaacta
     8341 tgtcctatca cattgaatag acatgtaatt aaagtattgc ttggtagaaa agtcaattgg
     8401 catgattttg ctttttttga tcctgtaatg tatgagagtt tgcggcaact aatcctcgcg
     8461 tctcagagtt cagatgctga tgctgttttc tcagcaatgg atttggcatt tgcaattgac
     8521 ctgtgtaaag aagaaggtgg aggacaggtt gaactcattc ctaatggtgt aaatatacca
     8581 gtcactccac agaatgtata tgagtatgtg cggaaatacg cagaacacag aatgttggta
     8641 gttgcagaac agcccttaca tgcaatgagg aaaggtctac tagatgtgct tccaaaaaat
     8701 tcattagaag atttaacggc agaagatttt aggcttttgg taaatggctg cggtgaagtc
     8761 aatgtgcaaa tgctgatcag ttttacctct ttcaatgatg aatcaggaga aaatgctgag
     8821 aagcttctgc agttcaagcg ttggttctgg tcaatagtag agaagatgag catgacagaa
     8881 cgacaagatc ttgtttactt ttggacatca agcccatcac tgccagccag tgaagaagga
     8941 ttccagccta tgccctcaat cacaataaga ccaccagatg accaacatct tcctactgca
     9001 aatacttgca tttctcgact ttacgtccca ctctattcct ctaaacagat tctcaaacag
     9061 aaattgttac tcgccattaa gaccaagaat tttggttttg tgctcgagta cccatacgac
     9121 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     9181 gtcccagact acgcatagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa
     9241 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9301 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9361 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9421 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9481 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9541 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9601 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     9661 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9721 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9781 ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa
     9841 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     9901 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     9961 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
    10021 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
    10081 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    10141 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10201 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10261 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg
    10321 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10381 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10441 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt
    10501 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    10561 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10801 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10861 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10921 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
    10981 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
    11041 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
    11101 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
    11161 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    11221 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    11281 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
    11341 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
    11401 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
    11461 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
    11521 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
    11581 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
    11641 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
    11701 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
    11761 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
    11821 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11881 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    11941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12061 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    12121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    12181 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    12241 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    12301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    12361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    12421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    12481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    12541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12661 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
    12721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12901 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    12961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    13021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    13081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    13141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    13201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    13261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    13321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    13381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    13441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    13501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    13561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    13981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    14041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    14101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    14161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    14221 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
    14281 tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
    14341 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
    14401 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
