LOCUS       V041505                13906 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041505
VERSION     V041505
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..8565
                     /label="USP24(NM_015306)"
                     /note="USP24(NM_015306)"
                     /gene="USP24"
     CDS             8572..8598
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             8602..8628
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             8632..8658
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    8682..9270
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9336..9384
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9586..10014
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10028..10357
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10424..11218
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11394..11515
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11564..11580)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11588..11604
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11612..11642)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11657..11678
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11966..12554)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12725..13585)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13586..13690)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga atcggaggag
      721 gagcagcaca tgaccacgct gctgtgcatg ggcttctcag accccgccac catccgcaag
      781 gccctgcgcc tggccaagaa cgacattaac gaggccgtgg cactgctcac caacgagcgg
      841 ccgggcctcg actacggcgg ctacgagccc atggacagcg gcggtggccc cagccccggg
      901 cccggcgggg gcccgcgggg cgacggcgga ggtgacggcg gcggcggcgg cccctcccgc
      961 ggcgggagca ccggaggcgg gggcggcttc gaccccccgc ccgcctacca cgaggtggtg
     1021 gacgcggaga agaatgatga gaatggaaac tgctcagggg aaggaattga attccctaca
     1081 acaaatttat atgaactgga aagccgtgtt ttgactgatc attggtccat cccttacaag
     1141 cgagaagaat cactaggcaa atgcctgttg gcatctacct acctagcaag acttggtctt
     1201 tccgagtctg atgagaattg tagaaggttt atggacaggt gtatgcctga agcatttaaa
     1261 aagctcctga catcaagtgc tgttcacaag tggggtactg aaattcatga aggaatttac
     1321 aacatgttga tgctattaat agaactggtc gcagagagaa taaaacaaga tccaattccc
     1381 actggtctcc tgggtgtgct tacaatggct ttcaatcctg ataatgaata ccattttaaa
     1441 aacagaatga aagtgtctca aaggaattgg gcagaagtgt ttggagaggg aaatatgttt
     1501 gctgtttcac ctgtatcgac tttccaaaag gagcctcatg gatgggttgt ggatttggta
     1561 aataagtttg gagaattagg tggatttgca gcaatccaag ccaagctcca ttcagaagat
     1621 atagaacttg gggctgtctc agcactgatt cagcccttag gagtgtgtgc agagtacctc
     1681 aattcctccg tggtacagcc catgctagac ccagtcattc ttactacaat ccaggatgta
     1741 cggagtgtag aagagaaaga cctcaaagac aagagattgg ttagcatccc tgagctcttg
     1801 tctgccgtta agttactttg catgcgcttc caaccggatc tggtgacaat tgtggatgac
     1861 cttcgactag atattctatt gcgcatgctg aaatcaccac atttcagtgc taagatgaat
     1921 tctctcaaag aagtaaccaa actaatagaa gatagcactt tatccaaatc tgtgaagaat
     1981 gctatagata cagacagatt attagattgg ctagttgaaa actcagttct gtcgattgca
     2041 ctggaaggca acatagacca agcacaatac tgtgaccgta taaagggaat tattgaactc
     2101 ttgggtagta aattgtcgtt agatgaactc actaaaattt ggaagataca gtcaggacaa
     2161 tcatctactg tgattgagaa cattcatact attattgctg cagcggctgt gaaatttaat
     2221 tcagatcagc ttaatcattt gtttgttctc attcagaaga gctgggagac tgagagtgat
     2281 agagtaagac agaagctttt gagcctgatt ggacgaatag gccgggaagc tcgctttgag
     2341 accacttctg gaaaggtttt agacgtactc tgggaactgg ctcaccttcc aaccctgccc
     2401 agtagcctta ttcagcaggc cttggaggag cacctgacaa tccttagtga tgcatatgca
     2461 gtgaaagaag caatcaagag gagctacatc atcaagtgca tagaagatat taagaggcct
     2521 ggagaatggt caggtttgga aaaaaacaag aaggatggat tcaagtcatc tcagcttaat
     2581 aatccccagt ttgtatgggt ggtaccagct ttgcgtcagc tccatgaaat tactcgctca
     2641 ttcataaaac aaacctatca aaagcaagac aagagcatta ttcaagactt gaagaagaat
     2701 tttgaaatag tgaaattggt aacgggaagt ttgatcgctt gtcatcggct tgcagctgct
     2761 gtggccgggc ctggaggctt aagtggctcg acactagtgg atggccggta cacttaccgg
     2821 gagtatttag aggcacatct aaaatttcta gcgtttttct tgcaagaagc tactctgtat
     2881 ctgggctgga atcgtgccaa ggagatctgg gagtgtcttg taactggcca ggatgtttgt
     2941 gaattagata gagagatgtg ttttgaatgg tttacaaaag gacagcatga tcttgagagt
     3001 gatgttcagc agcagctctt caaggagaaa attcttaaat tggagtcata tgaaatcact
     3061 atgaatggtt ttaacttatt taaaactttt tttgaaaatg tgaatctttg tgatcatcga
     3121 ttgaaaagac aaggagctca gttgtatgta gaaaagctgg aattgatagg aatggatttc
     3181 atttggaaaa tagccatgga atcacctgat gaagaaattg ctaatgaagc tattcagcta
     3241 atcataaact atagttacat taatctaaat cctagattaa agaaggattc agtatcttta
     3301 cataagaaat tcattgctga ttgctacaca agattagaag cagccagttc agcacttggt
     3361 ggccccactc taacacatgc tgtgaccaga gcaacaaaaa tgcttacagc aactgccatg
     3421 ccaactgtag caacctcagt tcagtctcct tatagatcta ctaaacttgt aataattgag
     3481 agattgctgc ttctggcaga gcgctatgtg atcactatag aggattttta ctctgttcca
     3541 cgaactattc tacctcatgg tgcctcattt catggacatc ttttaaccct taatgttacc
     3601 tatgagtcta ccaaagatac cttcactgtc gaggctcaca gtaatgaaac catagggagt
     3661 gtccggtgga aaatagccaa gcagttgtgc tctcctgtgg ataatataca gatatttaca
     3721 aatgatagcc tgctgacagt gaataaagat caaaagctac tccaccaact gggcttttct
     3781 gatgaacaaa tccttacagt gaagacttct ggcagtggga ccccatctgg gagttcagca
     3841 gattcttcaa ccagctccag cagcagcagc agtggggttt ttagttcttc atatgccatg
     3901 gagcaggaga aatccctccc tggtgtagtg atggctctcg tatgtaacgt atttgacatg
     3961 ctttatcagc tcgccaatct ggaagagcca aggataactc tacgagtacg gaagcttctg
     4021 ctcttgatac ccactgatcc agccattcag gaagcccttg atcaacttga ttctttagga
     4081 agaaagaaaa cattgctgtc tgaatcaagt tctcagtcct caaaatctcc atccctgtca
     4141 tcaaagcaac agcaccagcc aagtgccagt tcaattttag aaagtctgtt tcgatctttt
     4201 gccccgggaa tgtctacctt cagagtgctc tacaacttag aagttctaag ctccaaactc
     4261 atgccaacag ctgatgatga catggccaga agctgtgcca aatccttctg tgaaaacttc
     4321 ctcaaagctg gcggtttgag tttggttgta aatgtcatgc agagagactc catcccatca
     4381 gaagtagact atgaaacaag gcagggtgtt tattccatct gtctacagct tgcaagattt
     4441 ttacttgtcg gacaaacaat gcccacgtta ttagatgaag acctcaccaa agatggtata
     4501 gaagcacttt cttcccgccc attccgaaat gtcagccggc agacaagcag acagatgtcc
     4561 ttatgtggta ccccagaaaa gtcatcctac cgacagttgt ccgtgtctga taggtcttct
     4621 attagggttg aggaaatcat ccctgctgct cgagttgcaa tacaaacaat ggaagtaagt
     4681 gatttcactt ctactgtggc ttgcttcatg agattgtcat gggctgcggc tgcaggacgg
     4741 cttgatcttg ttgggagtag ccagccaatt aaagaaagta attccctgtg tcctgctgga
     4801 attcgaaaca gactcagcag ttcaggaagc aattgcagct ctggaagtga aggagaacca
     4861 gtagccctgc atgcgggaat ctgtgttcga caacagtctg tatccaccaa agactcgctg
     4921 attgcgggag aggctttgtc tcttcttgtt acgtgcctac agcttcggag ccagcaactg
     4981 gcatctttct ataacttgcc ctgtgttgct gatttcatca ttgatattct gctcggatca
     5041 ccaagtgctg agattcgccg ggttgcctgt gatcagctgt acactcttag tcagacagac
     5101 acatcagcgc atccagatgt gcagaagcca aatcagtttc ttctaggcgt aatcctcacg
     5161 gctcagctgc ctctctggtc tccaactagt attatgagag gagtcaatca gagactgtta
     5221 tctcagtgta tggagtattt tgatttgaga tgccagttat tagatgatct gacaacttca
     5281 gaaatggagc agttaaggat cagcccagct acgatgcttg aagatgagat tacttggctg
     5341 gataactttg aacctaatcg tacagctgaa tgtgagacca gtgaagcgga caacatctta
     5401 ctggcagggc acttacgcct catcaagacc cttctttcac tctgtggggc agaaaaggaa
     5461 atgcttggtt catcactcat taaaccattg ttagatgact tccttttccg agcttctaga
     5521 attattttaa atagtcattc tccagctggc agtgccgcca tcagtcaaca ggactttcat
     5581 ccaaagtgta gtacagcgaa tagccgattg gcagcctatg aagtccttgt gatgttggct
     5641 gatagttcac cttcaaatct tcaaattatt ataaaagaac tgctttctat gcatcaccag
     5701 cctgaccctg ctcttaccaa ggagtttgat taccttcccc cagtggatag caggtccagt
     5761 tcagggtttg tggggctgag aaatggtggt gcaacttgtt atatgaatgc agtcttccag
     5821 cagctgtata tgcaacctgg gctccctgag tcattacttt cagtggatga tgacacagac
     5881 aatccagatg atagcgtgtt ttaccaagtg cagtctctct ttggacattt aatggaaagc
     5941 aagctgcagt actatgtacc tgagaatttt tggaagattt tcaagatgtg gaataaagaa
     6001 ctttatgtga gagaacagca ggatgcatat gaattcttta ctagtctcat tgatcagatg
     6061 gatgaatacc tcaagaaaat ggggagagac caaattttta agaatacatt tcagggcatc
     6121 tactctgatc agaagatctg taaagactgt cctcacagat atgagcgtga agaagctttc
     6181 atggctctca atctaggagt gacttcttgt cagagtttgg aaatttcttt ggaccaattt
     6241 gttagaggag aagttctaga aggaagtaat gcgtactact gtgaaaagtg taaagaaaag
     6301 agaataacag tgaaaaggac ctgtattaaa tctttaccta gcgtcttggt aattcaccta
     6361 atgagatttg ggtttgactg ggaaagcgga cgctccatta aatatgatga acaaataagg
     6421 tttccctgga tgctaaacat ggagccttac acagtttcag gaatggctcg ccaagattct
     6481 tcttctgaag ttggggaaaa tgggcgaagt gtggatcagg gcggtggagg atccccacga
     6541 aaaaaggttg ccctcacaga aaactatgaa cttgtcggtg tcatcgtaca cagtgggcag
     6601 gcacacgcag gccactacta ttccttcatt aaggacaggc gagggtgtgg aaaaggaaag
     6661 tggtataaat ttaatgacac agttatagaa gaatttgacc taaatgacga gaccctggag
     6721 tatgaatgct ttggaggaga atatagacca aaagtttatg atcaaacaaa cccatacact
     6781 gatgtgcgcc gaagatactg gaatgcctat atgcttttct accaaagggt gtctgatcag
     6841 aactccccag tattaccaaa gaaaagtcga gtcagcgttg tacggcagga agctgaggat
     6901 ctctctctgt cagctccatc ttcaccagaa atttcacctc agtcatcccc tcggccccat
     6961 aggccgaaca atgaccggct gtctattctt accaagctgg ttaaaaaagg cgagaagaaa
     7021 ggactgtttg tggagaaaat gcctgctcga atataccaga tggtgagaga tgagaacctc
     7081 aagtttatga agaatagaga tgtatacagt agtgattatt tcagttttgt tttgtcttta
     7141 gcttcattga atgctactaa attaaagcat ccatattatc cttgcatggc aaaggtgagc
     7201 ttacagcttg ctattcaatt cctttttcaa acttatctac ggacaaagaa gaaactcagg
     7261 gttgatactg aagaatggat tgctaccatt gaagcattgc tttcaaaaag ttttgatgct
     7321 tgtcagtggt tagttgaata ttttattagt tctgaaggac gagaattgat aaagattttc
     7381 ttactggagt gcaatgtgag agaagtacga gttgctgtgg ccaccattct ggagaaaacc
     7441 ctagacagtg ccttgtttta tcaggataag ttaaaaagcc ttcatcagtt actggaggta
     7501 ctacttgctc tgttggacaa agacgtccca gaaaattgta aaaactgtgc tcagtacttt
     7561 ttcctgttca acacttttgt acaaaagcaa ggaattaggg ctggagatct tcttctgagg
     7621 cattcagctc tgcggcacat gatcagcttc ctcctagggg ccagtcggca aaacaatcag
     7681 atacgtcgat ggagttcagc acaagcacga gaatttggga atcttcacaa tacagtggcg
     7741 ttacttgttt tgcattcaga tgtctcatcc caaaggaatg ttgctcctgg catatttaag
     7801 caacgaccac ccattagcat tgctccctca agccctctgt tgcccctcca tgaggaggta
     7861 gaagccttgt tgttcatgtc tgaagggaaa ccttacctgt tagaggtaat gtttgctttg
     7921 cgggagctga caggctcgct cttggcactc attgagatgg tagtgtactg ctgtttctgt
     7981 aatgagcatt tttccttcac aatgctgcat ttcattaaga accaactaga aacggctcca
     8041 cctcatgagt taaagaatac gttccaacta cttcatgaaa tattggttat tgaagatcct
     8101 atacaagtag agcgagtcaa atttgtgttt gagacagaaa atggattact agctttgatg
     8161 caccacagta atcatgtgga cagtagtcgc tgctaccagt gtgtcaaatt tcttgtcact
     8221 cttgctcaaa agtgtcctgc agctaaggag tacttcaagg agaattccca ccactggagc
     8281 tgggctgtgc agtggctaca gaagaagatg tcagaacatt actggacacc acagagtaat
     8341 gtctctaatg aaacatcaac tggaaaaacc tttcagcgaa ccatttcagc tcaggacacg
     8401 ttagcgtatg ccacagcttt gttgaatgaa aaagagcaat caggaagcag taatgggtcg
     8461 gagagtagtc ctgccaatga gaacggagac aggcatctac agcagggttc agaatctccc
     8521 atgatgattg gtgagttgag aagtgacctt gatgatgttg atcccctcga gtacccatac
     8581 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat
     8641 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca
     8701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8761 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8821 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8881 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8941 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9001 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9061 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     9121 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9181 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9241 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
     9301 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     9361 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     9421 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     9481 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     9541 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9601 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9661 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9721 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     9781 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9841 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     9961 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
    10021 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10261 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    10321 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    10381 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
    10441 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
    10501 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
    10561 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
    10621 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    10681 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    10741 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    10801 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
    10861 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    10921 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
    10981 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    11041 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    11101 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    11161 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    11221 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
    11281 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    11341 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
    11401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    11461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11521 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
    11581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    11641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    11701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    11761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    11821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    11881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    12001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    12061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    12121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
    12181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    12241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    12301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    12361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    12421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    12481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    12541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    12601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    12661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    12721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    12781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    12841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    12901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    12961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    13021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    13081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    13141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    13201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    13261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    13321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    13381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    13441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    13501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    13561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    13621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    13681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    13741 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    13801 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    13861 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg
//
