LOCUS V031037 7608 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031037 VERSION V031037 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..3755 /label="Usp33(NM_133247)" /note="Usp33(NM_133247)" /gene="Usp33" CDS 3774..3803 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 3822..3845 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(3865..3881) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 3892..4514 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(4663..5251) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 5580..5627 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(5859..6653) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(6688..7045) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7047..7151) /gene="bla" /label="AmpR promoter" rep_origin 7178..7606 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gacgactttt cgaaatcatt gtccacattt ggattcagtt ggtgaaataa 1081 cgaaagagga cttgatacag aaatctctcg gcgcttgtca ggactgtaaa gtcagaggac 1141 caaacctgtg ggcgtgcctg gagaataggt gctcctatgt tggctgcggg gagtcgcaag 1201 tggaccacag caccatacac tcgcaggaga caaagcatta tctaactgtg aacctcacca 1261 ctcttcgagt atggtgttat gcttgcagca aagaagtatt tttggataga aaattaggaa 1321 ctcctccttc attacctcat gtaagacagc ctcaacaaac acaagaaaac agtgtccagg 1381 attttaaaat tcccagtaat ccagcattga aaacccccat ggttgctgtg tctgaagatc 1441 tggatataga agtggaagag gaagacgagc tgaaggctag aggcctgaca ggtttgaaaa 1501 acattggaaa tacttgttat atgaacgcgg cgctgcaggc tctttctaac tgcccacctt 1561 tgacacagtt ctttcttgat tgtggaggac tggctagaac agataagaaa ccagcaattt 1621 gtaaaagtta tctcaaacta atgaccgagt tgtggcacaa gagcaggcca ggatctgttg 1681 tgccggctaa tctgttccaa ggaattaaaa ctgtaaatcc aacttttcga ggttattctc 1741 agcaggatgc ccaggaattc cttcgctgtc taatggacct gcttcatgag gagctgaagg 1801 agcaggtcat ggaaatggag gaagagcctc aaacactaac ttctgaggag acggtggagg 1861 aagagaagag ccagtcagat gtggattttc agtcgtgcga gtcttgtagc agcagcgaga 1921 aagcagaaaa tgagagtggc tccaaaggct ttcctgagga cagcaatgag accaccatgc 1981 tcatccagga cgaggatgac ctggagatgg ccaaagactg gcagaaagag aaggtgtgca 2041 ataagatcaa caaggcaaat gccgatgtag aactggacaa agacagggac acagtgtgtg 2101 aaacagttga cctaaacagc caggagaccg tcaaagtgca gatacacggc agagcctcag 2161 aatctatcac tgatgtccat ctgaatgacc tagctacgtc acagatcctt ccttcaaatg 2221 aaagtgttag tccacggtta tcagcaagcc ctcctaagct aggcagtctg tggccaggac 2281 tgtcgcctcc acacaagaaa gctcagtcta catctgcaaa gaggaaaaag cagcataaga 2341 aatacagaag tgtcatctcc gacatattcg atggaacagt cattagctcg gtacagtgtc 2401 tgacgtgcga tagggtgtct ataaccctcg agacctttca ggatctgtcc ttgccgattc 2461 ctggcaagga ggacctggct aagctgcact cctccagtca cccaactata gtcaaagcag 2521 ggtcatgtgg tgaagcgtac gccccgcagg ggtggatagc tttcttcatg gagtatgtga 2581 agaggtttgt tgtctcatgt gtccctagct ggttttgggg tccagtagtt accttgcaag 2641 attgtcttgc tgccttcttc gccagagatg aacttaaagg tgacaacatg tacagttgtg 2701 aaaaatgcaa aaagttgagg aatggagtaa agttttgtaa agtacagaag tttcctgaga 2761 ttttgtgtat ccaccttaaa agatttcgac atgaactgat gttttccacc aaaattagca 2821 cccatgtttc cttccccctg gaaggcctcg atcttcagcc atttcttgca aaggacagcc 2881 cagctcagat tgtgacatat gatctcctgt cagtcatctg tcaccatggg actgcaagta 2941 gtgggcacta cattgcctac tgccgaaaca atttaaataa cctgtggtat gagtttgatg 3001 accagagcgt cactgaagtt tcagagtcca ctgtacagaa tgccgaggcc tacgtccttt 3061 tctacaggaa gagcagtgaa gaggcacaaa aggagaggcg gaggatatca aatttgttga 3121 acatcatgga acctagcctc cttcagttct acatatctcg acagtggttg aataaattta 3181 agacctttgc tgaacctggc cctatttcaa ataatgattt tctctgtatc catggaggta 3241 ttcctccacg aaaagcgagt tatattgaag acttagtttt gatgctgcct cagaacattt 3301 gggataacct ctatagcagg tatggaggag ggcctgctgt caaccatctc tacatctgcc 3361 acacctgcca aattgagtta gagaagattg aaaaacgaag aaaaaccgaa ttggaaattt 3421 ttattcggct caacagagca tttcaagagg aggactcccc agctactttt tactgtatca 3481 gcatgcagtg gtttagagaa tgggagagtt ttgtaaaggg taaggatgga gatcccccag 3541 gtccaatcga caacactaaa attgcggtta ctaaatgtgg cagtgtgatg ctcaagcaag 3601 gagcagactc tggtcaaatt tcagaagaaa catggaattt cctgcagtct atatatggtg 3661 gggggcctga agttatcctc cgacctccag ttgttcatgt tgaccctgat gtactccaag 3721 cagaggaaaa gattgaagta gaaactcgct ctttgacgcg tacgcggccg ctcgagcaga 3781 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg 3841 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat 3901 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc 3961 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat 4021 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg 4081 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca 4141 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc 4201 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc 4261 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc 4321 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa 4381 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg 4441 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag 4501 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt 4561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 4681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 4741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 4801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 4861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 4921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 4981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 5041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 5101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 5161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 5221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 5281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 5341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 5401 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc 5461 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca 5521 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg 5581 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt 5641 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa 5701 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc 5761 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg 5821 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg 5881 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg 5941 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga 6001 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc 6061 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc 6121 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc 6181 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt 6241 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca 6301 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc 6361 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct 6421 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca 6481 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc 6541 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc 6601 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac 6661 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc 6721 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa 6781 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg 6841 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct 6901 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct 6961 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt 7021 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat 7081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 7141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 7201 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 7261 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 7321 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 7381 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 7441 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 7501 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 7561 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt //