LOCUS       V035785                 9680 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035785
VERSION     V035785
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             775..1428
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1465..4386
                     /label="WDR59(NM_030581)"
                     /note="WDR59(NM_030581)"
                     /gene="WDR59"
     misc_feature    4456..5044
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5110..5158
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5360..5788
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5802..6131
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6198..6992
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7168..7289
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7338..7354)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7362..7378
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7386..7416)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7431..7452
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7740..8328)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8499..9359)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9360..9464)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc caccatgtcc
      781 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      841 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      901 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      961 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     1021 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     1081 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     1141 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     1201 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     1261 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     1321 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     1381 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaagg aggaggggga
     1441 agcggcggcg gagggagtga attcatggcg gcgcgatgga gcagcgaaaa cgtggttgta
     1501 gagttccgtg actcccaggc aactgcgatg tctgtggact gtcttgggca gcatgcagtg
     1561 ctttctggcc gcagattctt atacatcgtc aatctagatg cccctttcga aggtcaccga
     1621 aagatctctc gccagagcaa atgggacatt ggagctgtgc agtggaatcc tcatgacagc
     1681 tttgcacact attttgcggc ttcgagtaac caacgagtag acctttacaa gtggaaagac
     1741 ggcagtgggg aagttggcac aaccttacaa ggccacactc gtgtcatcag cgacttggac
     1801 tgggcggtgt ttgagcctga cctcctggtt accagctctg tggacaccta catctacatt
     1861 tgggatatca aagacacaag gaaacctact gttgcactgt ctgctgttgc gggtgcctcc
     1921 caggtcaaat ggaataaaaa aaatgctaac tgccttgcca ccagccatga cggcgatgtg
     1981 cggatatggg ataagaggaa acccagtaca gcagtggaat atctagccgc ccacctctcc
     2041 aaaatccatg gcctggactg gcacccagac agcgagcaca ttcttgctac ctccagtcaa
     2101 gacaattctg tgaagttctg ggattaccgc cagcctcgga aatacctcaa tattcttcct
     2161 tgccaggtgc ctgtctggaa ggccagatac acacctttca gcaatggatt ggtgactgtg
     2221 atggttcccc agctgcggag ggaaaacagc cttctcctgt ggaatgtctt tgacttgaac
     2281 accccagtcc acaccttcgt ggggcatgat gatgtggtcc tggagttcca gtggaggaag
     2341 cagaaggaag ggtccaagga ctatcaactg gtgacgtggt cccgggatca gaccttgaga
     2401 atgtggcggg tggattccca gatgcagagg ctttgtgcaa atgacatatt agatggtgtt
     2461 gatgagttca ttgagagtat ttcccttctg ccggaacctg agaagaccct gcacactgaa
     2521 gatacagatc accagcacac tgcaagccat ggggaggaag aagccctaaa agaagatccc
     2581 cctagaaatc tcctggaaga gaggaaatca gatcaactgg ggctgcctca gaccttgcag
     2641 caggaattct ccctgatcaa tgtgcaaatc cggaatgtca atgtggagat ggatgcggca
     2701 gacaggagct gcacagtgtc tgtgcactgc agcaaccatc gtgtcaagat gctggtgaag
     2761 ttccctgcac agtacccaaa caacgccgcc ccttccttcc agtttattaa ccccacaacc
     2821 atcacatcca ccatgaaagc taagctgctg aagatcctga aggacacagc cctgcagaaa
     2881 gtgaagcgtg gccagagctg cctggagccc tgcctgcgcc agctcgtctc ctgccttgag
     2941 tcctttgtga accaggaaga cagcgcttcc agcaacccgt ttgcactccc caactctgtc
     3001 actcccccct taccgacgtt tgcgcgggtg accacggctt acgggtcgta ccaggacgcc
     3061 aacattccct ttcctaggac ttctggggcc aggttctgcg gagcaggtta cctggtatat
     3121 ttcacaaggc ccatgacaat gcatcgggcg gtgtctccca cagagcctac tccgagatct
     3181 ctctcagcct tgtctgctta tcacactggc ttgatcgcgc ccatgaagat ccgcacagag
     3241 gcccctggga accttcgttt atacagtggg agccccactc gcagcgagaa agagcaggtc
     3301 tccatcagct ccttctacta caaggagcgg aaatcaagac gatggaaaag taagcgtgag
     3361 ggatcagact ctggcaatcg acagatcaag gctgctggga aagtcatcat ccaggatatt
     3421 gcttgcctcc tgcctgttca caaatcgctg ggagagctgt acatattgaa tgtgaatgat
     3481 attcaggaaa catgtcagaa gaatgccgcc tctgccttgc tcgttggaag aaaggatctt
     3541 gtccaggttt ggtcgctggc tacggtagct acagatcttt gccttggtcc gaaatctgac
     3601 ccagatttgg aaacaccctg ggctcgacat ccatttgggc ggcagctgct ggagtccctg
     3661 ttggctcact attgccggct ccgggatgtt cagacactgg cgatgctctg tagcgtgttt
     3721 gaagcccagt ctcggcctca ggggctacca aacccctttg ggccttttcc taaccgttct
     3781 tctaatcttg tggtgtccca tagtcgatat cctagcttta cctcttctgg ttcctgctcc
     3841 agtatgtcag acccagggct caacactggc ggctggaaca tagcgggaag agaggcagag
     3901 cacttgtcct ccccttgggg agaatcctca ccagaagagc tccgctttgg gagtctgacc
     3961 tacagtgatc cccgtgagcg agaacgcgac cagcatgata aaaataaaag gctcctggac
     4021 cccgccaata cccagcaatt tgatgacttt aagaaatgct atggggaaat cctctaccgt
     4081 tggggtctga gagagaagcg agctgaagtg ttgaagtttg tctcctgtcc tcctgaccct
     4141 cacaaaggga tcgagttcgg cgtgtactgc agccactgcc ggagtgaggt ccgtggcacg
     4201 cagtgtgcca tctgcaaagg cttcacgttc cagtgtgcca tctgtcacgt ggctgtgcgg
     4261 ggatcgtcca atttctgcct gacctgtggg cacggtggcc acaccagcca catgatggag
     4321 tggtttcgga cccaggaggt gtgtcccacc gggtgtgggt gccactgcct gcttgaaagc
     4381 actttctgac tcgagtctag agggcccctt aagggttcga tccctaccgg ttagtaatga
     4441 gtttgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4861 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5041 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     5101 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     5161 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5221 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5281 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg
     5341 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5401 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5461 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     5521 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5581 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5641 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5701 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     5761 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5881 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5941 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6061 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6121 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6181 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6241 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6301 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6361 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6421 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6481 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6541 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6601 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6661 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6721 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6781 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6841 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6901 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6961 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc
     7021 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7081 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7141 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7261 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7921 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9481 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta
     9541 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9601 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9661 gcatgaagaa tctgcttagg
//
