LOCUS V028397 7969 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028397 VERSION V028397 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2628 /label="Wdr26(NM_145514)" /note="Wdr26(NM_145514)" /gene="Wdr26" CDS 2635..2661 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2665..2691 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2695..2721 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2745..3333 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3399..3447 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3649..4077 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4091..4420 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4487..5281 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5457..5578 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5627..5643) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5651..5667 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5675..5705) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5720..5741 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6029..6617) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6788..7648) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7649..7753) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgca ggccaacggg 721 gcgggaggag gcggcggcgg gggcggcggg ggccagggcc agaccccgga gctcgcctgc 781 ctgtcggcgc agaacgggga gtcgtcccct tcggcgacgt ccgcggggga cctggcccac 841 gccaacgggc tcctgcctgc cgccccctcc gccgctggca acaatagcaa cagcctgagt 901 gtcaataacg gggttcccgg cggggccgcc gcggcctccg ccaccgccgc cgccgcccag 961 gccacccccg agctgggcag cagcctcaag aagaagaagc ggctctcgca gtccgatgag 1021 gatgtcatta ggctcatagg acagcacctc aatggcctag ggctcaacca gactgttgat 1081 ctcctcatgc aagagtcagg atgtcgttta gagcatcctt ctgctaccaa attccgaaat 1141 catgtcatgg aaggagactg ggataaggca gagaatgacc tgaatgagct aaagccttta 1201 gtgcattctc ctcacgctat tgtggtaaga ggcgcacttg aaatctctca aacgttgttg 1261 ggaataattg tgaggatgaa gtttctgctg ctgcagcaga agtacctgga atacctggag 1321 gacggcaagg tcctggaggc acttcaagtt ctacgctgcg aactgacgcc gttgaaatac 1381 aacaccgagc gcatccatgt ccttagtggg tatctgatgt gcagccatgc cgaagaccta 1441 cgggcaaaag ctgaatggga aggcaagggc acagcgtccc ggtccaaact gctggacaag 1501 cttcagacct atttaccacc atcagtgatg cttcccccac ggcgtttaca gactctcctg 1561 cggcaggcgg tggaactaca aagggatcgg tgcctatatc acaataccaa acttgacaat 1621 aatctagatt ctgtgtctct acttatagat catgtttgta gtaggaggca gtttccctgt 1681 tacactcaac agatacttac agagcattgt aatgaagtgt ggttctgtaa attttctaat 1741 gatggcacta aactagcaac aggatcaaag gataccacag ttatcatatg gcaagttgat 1801 ccggatacgc acctgttaaa actgcttaaa acgttagaag gacatgcgta tggtgtctct 1861 tatatagcat ggagtccaga tgacagctat cttgttgctt gtggtccaga tgactgctct 1921 gagctttggc tttggaatgt acagacggga gaattaagaa caaaaatgag ccaatctcat 1981 gaagacagtt tgaccagtgt ggcctggaat ccagatggga aacgctttgt gacgggaggt 2041 cagcgtggtc agttctacca gtgtgacctg gacggaaatc ttctggactc ctgggaagga 2101 gtgcgagtac agtgcctgtg gtgcttgagt gacgggaaga ctgtgctggc ttccgacacg 2161 caccagagag tccggggcta caactttgag gacctgacag atagaaacat agtacaggaa 2221 gatcatccta ttatgtcatt tactatttcc aaaaatggcc gattagcttt gttaaatgta 2281 gcaactcagg gagttcattt atgggacttg caagacagag ttttagtaag gaaatatcaa 2341 ggtgttaccc aagggtttta tacaatccac tcgtgttttg gaggccataa tgaagacttc 2401 attgctagtg gcagcgaaga tcacaaggtt tacatctggc acaaacgtag tgaactgcca 2461 attgcggagc tcacagggca cacgcgcaca gtaaattgtg ttagctggaa cccacagatt 2521 ccatccatga tggccagtgc ctcagacgat ggcactgtta gaatatgggg accagcacct 2581 ttcatagacc accagaatat tgaagaggaa tgcagtagca tggatagtct cgagtaccca 2641 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct 2701 tatgatgtcc cagactacgc atagtgaggg cccgatatct cgacaatcaa cctctggatt 2761 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 2821 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2881 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 2941 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 3001 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 3061 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 3121 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 3181 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 3241 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 3301 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac 3361 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 3421 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 3481 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 3541 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 3601 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta 3661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 3721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 3781 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 3841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 3901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 3961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 4021 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 4081 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 4141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 4201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 4261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 4321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 4381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 4441 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 4501 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4561 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4621 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 4681 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4741 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4801 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4861 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4921 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4981 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 5041 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 5101 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 5161 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 5221 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 5281 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 5341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 5401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 5461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 5521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 5581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 5641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 5701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 5881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 5941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 6241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 7681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 7801 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 7861 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 7921 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg //