LOCUS       40924_19141        4822 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA-TagRFP-T-CmLEU2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida 
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur 
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 4822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield, 
            Montreal, Quebec H3A 1B1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..786
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     regulatory      792..1045
                     /label=Saccharomyces cerevisiae URA3 terminator
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1214..2332
                     /gene="LEU2"
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     promoter        complement(2460..2478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3498..4355)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4356..4460)
                     /label=AmpR promoter
     promoter        4806..4822
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttcggta
       61 tctaagggtg aagaattgat taaggaaaac atgcacatga agttgtatat ggaaggtact
      121 gtaaataacc accattttaa gtgtacctct gaaggtgaag gtaaaccata tgaaggtaca
      181 caaaccatga gaattaaagt tgtagaaggt ggtccattgc cttttgcctt cgatatctta
      241 gctacatctt ttatgtacgg ttcaagaacc ttcatcaatc atactcaagg tataccagat
      301 ttctttaagc aatcattccc tgaaggtttc acttgggaaa gagtcactac atatgaagac
      361 ggtggtgttt tgactgccac acaagataca tccttgcaag acggttgttt aatctataac
      421 gttaagatca gaggtgtaaa cttcccaagt aacggtcctg taatgcaaaa gaaaactttg
      481 ggttgggaag caaacaccga aatgttgtat cctgccgatg gtggtttaga aggtagaact
      541 gacatggcat tgaaattagt tggtggtggt cacttaattt gcaatttcaa gaccacttac
      601 agatctaaaa agccagctaa aaacttgaag atgcctggtg tatactacgt cgatcataga
      661 ttggaaagaa ttaaagaagc agacaaggaa acatatgttg aacaacacga agtcgctgtt
      721 gcaagatact gcgatttgcc ttccaagtta ggtcataagt tgaacggtat ggacgaattg
      781 tataagtaag gcgcgccatt ataagtaaat gcatgtatac taaactcaca aattagagct
      841 tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ctataatgac
      901 gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat
      961 acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg
     1021 actgtaaaga tggacgcata gatccgtaac ggcgtgcgtg ggttccgcag ccgggttcaa
     1081 tttttttttt cttgttcact ataaaaatat ataataaacg aaaaaaatcc gagggactca
     1141 ggctatgaaa tttttcactc aagaattttt tttccttcat ccttaattcc tttattcact
     1201 acccaaaccc acaatgtcag ttaaaactaa aactattaca attttaccag gtgatcacgt
     1261 cggtaccgaa attgtcaatg aagctatcaa agttttagaa gcaattgaag ctgccactcc
     1321 ttaccaaaaa atccatttcg atttcaaaca tcatttaatc ggtggtgctg ccattgatgc
     1381 cactggtgtc ccattgccag atgacgcact tgaaagtgcc aaaaaatctg atgccgtctt
     1441 attgggtgct gttggtggac caaaatgggg gactggcgcc gttcgtccag aacaaggttt
     1501 attaaagatt cgtaaagaat tgaaccttta tgctaacatc agaccatgta actttgccag
     1561 tgactcctta ttggaattat ccccattgag accagaagtc gttaaaggta caaacttgat
     1621 cattgttcgt gaattagttg gtggtattta tttcggtgat cgtgaagaac aagaagaaag
     1681 tgaagataaa caaaccgcat gggatactga aaaatacacc gtcgatgaag tcaccagaat
     1741 cacccgtatg gctgccttca tggctttaca acataaccca ccattaccaa tctggtcatt
     1801 ggataaggct aatgttttag cttcttctag attatggaga agaactgtcg ataaagtgat
     1861 ttctgaagag ttcccaactt tgtctgttca acatcaattg attgactccg ccgccatgat
     1921 tttaattcaa aacccaacca aattgaatgg tataatcatc acttctaaca tgtttggtga
     1981 tatcatttcc gatgaagctt cagttattcc aggttccttg ggtttattac catctgcatc
     2041 tttggcttca ttgccagata ccaacaccgc ttttggtctt tatgaaccat gtcacggttc
     2101 tgctcctgat ttaccagcca ataaagttaa cccaattgct actattttat ctgctgcttc
     2161 tatgttgaga ttatctttgg attgtgtgaa agaagctgaa gctttagaag aagccgtgaa
     2221 acaagtcttg gataaaggta tcagaactgc agatttaaga ggtaccagtt cagctactga
     2281 agttggtgac gcgattgtcg aagctgttac taaaatctta aaagaaaaag cttaagtaaa
     2341 gtatacacaa aatttaacga atattattaa tacaaaatga acaccgttta aacgagctcg
     2401 aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc
     2461 ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa
     2521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4501 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4561 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4621 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4681 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4741 atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     4801 atacgattta ggtgacacta ta
//
