LOCUS       pFA6a-TRP1-PGAL1        4881 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker for swapping in the GAL1 promoter and 
            adding a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-TRP1-PGAL1-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4881)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4881)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(261..971)
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     CDS             complement(984..986)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(1006..1447)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        1542..1689
                     /label=TRP1 promoter
     CDS             1690..2361
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2519..2537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2795..3383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3557..4414)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4415..4519)
                     /label=AmpR promoter
     promoter        4865..4881
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcccta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      301 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      361 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      421 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      481 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      541 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      601 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      661 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      721 ttgaaaaagt catgccgttt catatgatct gggtatcttg aaaagcattg aacaccatac
      781 gtaaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      841 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      901 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct
      961 tctcctttac tgttaattaa agacattttg agatccgggt tttttctcct tgacgttaaa
     1021 gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa taagaagtaa
     1081 tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt gcatttatat
     1141 atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
     1201 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
     1261 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
     1321 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
     1381 agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct cggcggcttc
     1441 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
     1501 aggttttttt aggctaagat aatggggctc tttacagatc tgtacaatct tgatccggag
     1561 cttttctttt tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa
     1621 gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca
     1681 gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc
     1741 ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt
     1801 attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca
     1861 agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt
     1921 cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc
     1981 caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt
     2041 aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct
     2101 cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg
     2161 aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta
     2221 gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt
     2281 gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat
     2341 ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg
     2401 tgcacttgcc tgcggtgtga aataccgcac agatgcgtaa ggaggtttaa acgagctcga
     2461 attcatcgat gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc
     2521 tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac
     2581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4561 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     4621 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4681 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4741 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4801 tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     4861 tacgatttag gtgacactat a
//
